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Infection and Immunity, June 2006, p. 3684-3686, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01521-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Development of Luminescent Mycobacterium avium subsp. paratuberculosis for Rapid Screening of Vaccine Candidates in Mice

Valérie Rosseels,1 Virginie Roupie,1 Denise Zinniel,2 Raúl G. Barletta,2 and Kris Huygen1*

Mycobacterial Immunology, WIV-Pasteur Institute Brussels, 642 Engelandstraat, B-1180 Brussels, Belgium,1 Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska 68583-09052

Received 15 September 2005/ Returned for modification 9 November 2005/ Accepted 27 March 2006

Mycobacterium avium subsp. paratuberculosis is a slowly growing mycobacterial species, requiring 6 to 8 weeks of culture before colonies can be counted visually. Here, we describe the development of luminescent M. avium subsp. paratuberculosis expressing luxAB genes of Vibrio harveyi and its use for vaccine testing in an experimental mouse model, replacing fastidious CFU counting by rapid luminometry.

* Corresponding author. Mailing address: WIV-Pasteur Institute Brussels, 642 Engelandstraat, B-1180 Brussels, Belgium. Phone: 32 2 373 33 70. Fax: 32 2 373 33 67. E-mail: khuygen{at}pasteur.be.

Editor: J. L. Flynn

Infection and Immunity, June 2006, p. 3684-3686, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.01521-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roupie, V., Rosseels, V., Piersoel, V., Zinniel, D. K., Barletta, R. G., Huygen, K. (2008). Genetic Resistance of Mice to Mycobacterium paratuberculosis Is Influenced by Slc11a1 at the Early but Not at the Late Stage of Infection. Infect. Immun. 76: 2099-2105 [Abstract] [Full Text]  


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