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Infection and Immunity, August 2006, p. 4566-4572, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.01660-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Immunogenicity of Mycobacterium tuberculosis Antigens in Mycobacterium bovis BCG-Vaccinated and M. bovis-Infected Cattle

A. S. Mustafa,1 Y. A. Skeiky,3,{dagger} R. Al-Attiyah,1 M. R. Alderson,4 R. G. Hewinson,2 and H. M. Vordermeier2*

Department of Microbiology, Faculty of Medicine, Kuwait University, Safat 13110, Kuwait,1 VLA Weybridge, Department of Bacterial Disease, TB Research Group, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom,2 Corixa Corporation, Seattle, Washington,3 GlaxoSmithKline Biologicals, Seattle, Washington4

Received 11 October 2005/ Returned for modification 14 December 2005/ Accepted 1 May 2006

The development of novel vaccine strategies supplementing Mycobacterium bovis BCG (BCG) constitutes an urgent research challenge. To identify potential subunit vaccine candidates, we have tested a series of eight recently identified Mycobacterium tuberculosis antigens in M. bovis-infected and BCG-vaccinated cattle. These antigens were characterized on the basis of their ability to induce in vitro gamma interferon responses in infected or BCG-vaccinated calves. We were able to establish a hierarchy of these antigens based on how frequently they were recognized in both groups of animals. In particular, we were able to prioritize frequently recognized proteins like Rv0287, Rv1174, and Rv1196 for future evaluation as subunit vaccines to be used in BCG-protein heterologous prime-boost vaccination scenarios. In addition, the antigen most dominantly recognized in M. bovis-infected cattle in this study, Rv3616c, was significantly less frequently recognized by BCG vaccinees and could be a target to improve BCG, for example, by increasing its secretion, in a recombinant BCG vaccine.

* Corresponding author. Mailing address: TB Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom. Phone: 44 1932 357 884. Fax: 44 1932 347046. E-mail: m.vordermeier{at}vla.defra.gsi.gov.uk.

Editor: J. L. Flynn

{dagger} Current address: Aeras Global TB Vaccine Foundation, Bethesda, MD 20814.

Infection and Immunity, August 2006, p. 4566-4572, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.01660-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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  • Mustafa, A. S., Al-Attiyah, R., Hanif, S. N. M., Shaban, F. A. (2008). Efficient Testing of Large Pools of Mycobacterium tuberculosis RD1 Peptides and Identification of Major Antigens and Immunodominant Peptides Recognized by Human Th1 Cells. CVI 15: 916-924 [Abstract] [Full Text]  


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