This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Runyen-Janecky, L.
Right arrow Articles by Warner, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Runyen-Janecky, L.
Right arrow Articles by Warner, L.

 Previous Article  |  Next Article 

Infection and Immunity, August 2006, p. 4666-4672, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00562-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Role and Regulation of the Shigella flexneri Sit and MntH Systems

Laura Runyen-Janecky,* Ellyn Dazenski, Stephanie Hawkins, and Lisa Warner

Department of Biology, University of Richmond, Richmond, Virginia 23173

Received 5 April 2006/ Returned for modification 10 May 2006/ Accepted 30 May 2006

Shigella flexneri possesses at least two putative high-affinity manganese acquisition systems, SitABCD and MntH. Mutations in the genes encoding the components of both of these systems were constructed in S. flexneri. The sitA mntH mutant showed reduced growth, relative to the wild type, in Luria broth (L broth) containing the divalent metal chelator ethylene diamino-o-dihydroxyphenyl acetic acid, and the addition of either iron or manganese restored growth to the level of the wild-type strain. Although the sitA mntH mutant was not defective in surviving exposure to superoxide generators, it was defective in surviving exposure to hydrogen peroxide. The sitA mntH mutant formed wild-type plaques on Henle cell monolayers but had a reduced ability to survive in activated macrophage lines. Expression of the S. flexneri sit and mntH promoters was higher when Shigella was in Henle cells than when it was in L broth. Expression of both the sit and mntH promoters was repressed by either iron or manganese, and this repression was partially dependent upon Fur and MntR, respectively. The mntH promoter, but not the sit promoter, exhibited OxyR-dependent induction in the presence of hydrogen peroxide.

* Corresponding author. Mailing address: Department of Biology, University of Richmond, Richmond, VA 23173. Phone: (804) 287-6390. Fax: (804) 289-8233. E-mail: lrunyenj{at}richmond.edu.

Editor: D. L. Burns

Infection and Immunity, August 2006, p. 4666-4672, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00562-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Anderson, E. S., Paulley, J. T., Gaines, J. M., Valderas, M. W., Martin, D. W., Menscher, E., Brown, T. D., Burns, C. S., Roop, R. M. II (2009). The Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice. Infect. Immun. 77: 3466-3474 [Abstract] [Full Text]  
  • Fisher, C. R., Davies, N. M. L. L., Wyckoff, E. E., Feng, Z., Oaks, E. V., Payne, S. M. (2009). Genetics and Virulence Association of the Shigella flexneri Sit Iron Transport System. Infect. Immun. 77: 1992-1999 [Abstract] [Full Text]  
  • Johnson, T. J., Wannemuehler, Y. M., Nolan, L. K. (2008). Evolution of the iss Gene in Escherichia coli. Appl. Environ. Microbiol. 74: 2360-2369 [Abstract] [Full Text]  
  • Runyen-Janecky, L., Daugherty, A., Lloyd, B., Wellington, C., Eskandarian, H., Sagransky, M. (2008). Role and Regulation of Iron-Sulfur Cluster Biosynthesis Genes in Shigella flexneri Virulence. Infect. Immun. 76: 1083-1092 [Abstract] [Full Text]  
  • Sabri, M., Caza, M., Proulx, J., Lymberopoulos, M. H., Bree, A., Moulin-Schouleur, M., Curtiss, R. III, Dozois, C. M. (2008). Contribution of the SitABCD, MntH, and FeoB Metal Transporters to the Virulence of Avian Pathogenic Escherichia coli O78 Strain {chi}7122. Infect. Immun. 76: 601-611 [Abstract] [Full Text]  
  • Boulette, M. L., Payne, S. M. (2007). Anaerobic Regulation of Shigella flexneri Virulence: ArcA Regulates fur and Iron Acquisition Genes. J. Bacteriol. 189: 6957-6967 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»