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Infection and Immunity, August 2006, p. 4757-4765, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00265-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Characterization of the T-Cell Receptor Vß Repertoire in the Human Immune Response against Leishmania Parasites

Jorge Clarêncio,1* Camila I. de Oliveira,1 Glória Bomfim,2 Margarida M. Pompeu,3 Maria Jania Teixeira,3 Theolis C. Barbosa,1 Sebastião Souza-Neto,1 Edgar M. Carvalho,2,5 Cláudia Brodskyn,1,4,5 Aldina Barral,1,2,5 and Manoel Barral-Netto1,2,5

Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão, 121, Salvador, Bahia 40296-710, Brazil,1 Faculdade de Medicina, UFBA, Praça XV de Novembro, S/N, Largo do Terreiro de Jesus Salvador, Bahia 40025-010, Brazil,2 Núcleo de Medicina Tropical, UFC, Rua Alexandre Baraúna, 949, Fortaleza, Ceara 60430-160, Brazil,3 Departamento de Biointeração, ICS, UFBA, Av. Reitor Miguel Calmon, S/N, Salvador, Buenos Aires 40110-160, Brazil,4 Instituto de Investigação em Imunologia, São Paulo, Brazil5

Received 17 February 2006/ Returned for modification 17 March 2006/ Accepted 16 May 2006

In order to explore a possible presence of hyperreactive T-cell clones in human cutaneous leishmaniasis (CL), we have investigated, by flow cytometry, the expression of Vß chains of T-cell receptors (TCRs) in the following types of cells: (i) peripheral blood mononuclear cells (PBMCs) from CL patients, which were then compared to those from normal volunteers; (ii) unstimulated and soluble Leishmania antigen-stimulated draining lymph node cells from CL patients; (iii) PBMCs from volunteers before versus after Leishmania immunization; and (iv) PBMCs from healthy volunteers that were primed in vitro with live Leishmania parasites. Our results show a modulation in the TCR Vß repertoire during CL and after antigen stimulation of patients' cells. Vaccination, however, leads to a broad expansion of different Vß TCRs. We also observed an association between TCR Vß12 expression, T-cell activation, and gamma interferon production upon in vitro priming with Leishmania. Collectively, these results both indicate that infection with live parasites or exposure to parasite antigen can modulate the TCR Vß repertoire and suggest that TCR Vß12 may be implicated in the response to Leishmania.

* Corresponding author. Mailing address: Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão, 121, Salvador, Bahia 40296-710, Brazil. Phone: 55-71-3176 2261. Fax: 55-71-3176 2279. E-mail: jorgec{at}cpqgm.fiocruz.br.

Editor: W. A. Petri, Jr.

Infection and Immunity, August 2006, p. 4757-4765, Vol. 74, No. 8
0019-9567/06/$08.00+0     doi:10.1128/IAI.00265-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.