This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raynaud, C.
Right arrow Articles by Charbit, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raynaud, C.
Right arrow Articles by Charbit, A.

 Previous Article  |  Next Article 

Infection and Immunity, January 2007, p. 536-541, Vol. 75, No. 1
0019-9567/07/$08.00+0     doi:10.1128/IAI.01429-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of the wbt Locus of Francisella tularensis in Lipopolysaccharide O-Antigen Biogenesis and Pathogenicity{triangledown}

Catherine Raynaud,1,2,{dagger} Karin L. Meibom,1,2,{dagger} Marie-Annick Lety,1,2,{dagger} Iharilalao Dubail,1,2 Thomas Candela,3 Eric Frapy,1,2 and Alain Charbit1,2,{dagger}*

Université Paris Descartes, Faculté de Médecine René Descartes, Paris F-75015, France,1 Inserm, U570, Unité de Pathogénie des Infections Systémiques, Paris F-75015, France,2 Unité Toxines et Pathogénie Bactérienne CNRS-URA2172, 28 rue de Dr Roux, 75724 Paris Cedex 15, France3

Received 5 September 2006/ Accepted 18 September 2006

Francisella tularensis is a highly infectious bacterial pathogen, responsible for the zoonotic disease tularemia. We screened a bank of transposon insertion mutants of F. tularensis subsp. holarctica LVS for colony morphology alterations and selected a mutant with a transposon insertion in wbtA, the first gene of the predicted lipopolysaccharide O-antigen gene cluster. Inactivation of wbtA led to the complete loss of O antigen, conferred serum sensitivity, impaired intracellular replication, and severely attenuated virulence in the mouse model. Notably, this mutant afforded protection against a challenge against virulent LVS.

* Corresponding author. Present address: Faculté de Médecine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France. Phone: 33 1 40 61 53 76. Fax: 33 1 40 61 55 92. E-mail: charbit{at}necker.fr.

{triangledown} Published ahead of print on 9 October 2006.

Editor: D. L. Burns

{dagger} C.R., K.L.M., M.-A.L., and A.C. contributed equally to this work.

Infection and Immunity, January 2007, p. 536-541, Vol. 75, No. 1
0019-9567/07/$08.00+0     doi:10.1128/IAI.01429-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Clay, C. D., Soni, S., Gunn, J. S., Schlesinger, L. S. (2008). Evasion of Complement-Mediated Lysis and Complement C3 Deposition Are Regulated by Francisella tularensis Lipopolysaccharide O Antigen. J. Immunol. 181: 5568-5578 [Abstract] [Full Text]  
  • Oyston, P. C. F. (2008). Francisella tularensis: unravelling the secrets of an intracellular pathogen. J Med Microbiol 57: 921-930 [Abstract] [Full Text]  
  • Craven, R. R., Hall, J. D., Fuller, J. R., Taft-Benz, S., Kawula, T. H. (2008). Francisella tularensis Invasion of Lung Epithelial Cells. Infect. Immun. 76: 2833-2842 [Abstract] [Full Text]  
  • Goebel, E. M., Wolfe, D. N., Elder, K., Stibitz, S., Harvill, E. T. (2008). O Antigen Protects Bordetella parapertussis from Complement. Infect. Immun. 76: 1774-1780 [Abstract] [Full Text]  
  • Maier, T. M., Casey, M. S., Becker, R. H., Dorsey, C. W., Glass, E. M., Maltsev, N., Zahrt, T. C., Frank, D. W. (2007). Identification of Francisella tularensis Himar1-Based Transposon Mutants Defective for Replication in Macrophages. Infect. Immun. 75: 5376-5389 [Abstract] [Full Text]  
  • Su, J., Yang, J., Zhao, D., Kawula, T. H., Banas, J. A., Zhang, J.-R. (2007). Genome-Wide Identification of Francisella tularensis Virulence Determinants. Infect. Immun. 75: 3089-3101 [Abstract] [Full Text]  
  • Sebastian, S., Dillon, S. T., Lynch, J. G., Blalock, L. T., Balon, E., Lee, K. T., Comstock, L. E., Conlan, J. W., Rubin, E. J., Tzianabos, A. O., Kasper, D. L. (2007). A Defined O-Antigen Polysaccharide Mutant of Francisella tularensis Live Vaccine Strain Has Attenuated Virulence while Retaining Its Protective Capacity. Infect. Immun. 75: 2591-2602 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»