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Infection and Immunity, November 2007, p. 5353-5360, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00922-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Modulation of Host Innate Immune Response in the Bladder by Uropathogenic Escherichia coli{triangledown}

Benjamin K. Billips,1 Sarah G. Forrestal,1 Matthew T. Rycyk,1,{dagger} James R. Johnson,2 David J. Klumpp,1,3* and Anthony J. Schaeffer1

Departments of Urology,1 Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 16-703 Tarry, 303 East Chicago Avenue, Chicago, Illinois 60611,3 Mucosal and Vaccine Research Center, Infectious Diseases, Room 3B-101, VA Medical Center, One Veterans Drive, Minneapolis, Minnesota 554172

Received 6 July 2007/ Returned for modification 6 August 2007/ Accepted 16 August 2007

Uropathogenic Escherichia coli (UPEC), the most frequent cause of urinary tract infection (UTI), is associated with an inflammatory response which includes the induction of cytokine/chemokine secretion by urothelial cells and neutrophil recruitment to the bladder. Recent studies indicate, however, that UPEC can evade the early activation of urothelial innate immune response in vitro. In this study, we report that infection with the prototypic UPEC strain NU14 suppresses tumor necrosis factor alpha (TNF-{alpha})-mediated interleukin-8 (CXCL-8) and interleukin-6 (CXCL-6) secretion from urothelial cell cultures compared to infection with a type 1 piliated E. coli K-12 strain. Furthermore, examination of a panel of clinical E. coli isolates revealed that 15 of 17 strains also possessed the ability to suppress cytokine secretion. In a murine model of UTI, NU14 infection resulted in diminished levels of mRNAs encoding keratinocyte-derived chemokine, macrophage inflammatory peptide 2, and CXCL-6 in the bladder relative to infection with an E. coli K-12 strain. Furthermore, reduced stimulation of inflammatory chemokine production during NU14 infection correlated with decreased levels of bladder and urine myeloperoxidase and increased bacterial colonization. These data indicate that a broad phylogenetic range of clinical E. coli isolates, including UPEC, may evade the activation of innate immune response in the urinary tract, thereby providing a pathogenic advantage.

* Corresponding author. Mailing address: Departments of Urology and Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 16-703 Tarry, 303 East Chicago Avenue, Chicago, IL 60611. Phone: (312) 908-1996. Fax: (312) 908-7275. E-mail: d-klumpp{at}northwestern.edu

{triangledown} Published ahead of print on 27 August 2007.

Editor: A. J. Bäumler

{dagger} Present address: Reddy US Therapeutics, Inc., 3065 Northwoods Circle, Norcross, GA 30071-1542.

Infection and Immunity, November 2007, p. 5353-5360, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00922-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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