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Infection and Immunity, December 2007, p. 5575-5585, Vol. 75, No. 12
0019-9567/07/$08.00+0     doi:10.1128/IAI.00443-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sel1 Repeat Protein LpnE Is a Legionella pneumophila Virulence Determinant That Influences Vacuolar Trafficking{triangledown}

Hayley J. Newton,1 Fiona M. Sansom,1 Jenny Dao,2 Adrian D. McAlister,1 Joan Sloan,1 Nicholas P. Cianciotto,2 and Elizabeth L. Hartland1,3*

Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia,1 Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611,2 Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia3

Received 26 March 2007/ Returned for modification 10 May 2007/ Accepted 5 September 2007

The environmental pathogen Legionella pneumophila possesses five proteins with Sel1 repeats (SLRs) from the tetratricopeptide repeat protein family. Three of these proteins, LpnE, EnhC, and LidL, have been implicated in the ability of L. pneumophila to efficiently establish infection and/or manipulate host cell trafficking events. Previously, we showed that LpnE is important for L. pneumophila entry into macrophages and epithelial cells. In further virulence studies here, we show that LpnE is also required for efficient infection of Acanthamoeba castellanii by L. pneumophila and for replication of L. pneumophila in the lungs of A/J mice. In addition, we found that the role of LpnE in host cell invasion is dependent on the eight SLR regions of the protein. A truncated form of LpnE lacking the two C-terminal SLR domains was unable to complement the invasion defect of an lpnE mutant of L. pneumophila 130b in both the A549 and THP-1 cell lines. The lpnE mutant displayed impaired avoidance of LAMP-1 association, suggesting that LpnE influenced trafficking of the L. pneumophila vacuole, similar to the case for EnhC and LidL. We also found that LpnE was present in L. pneumophila culture supernatants and that its export was independent of both the Lsp type II secretion system and the Dot/Icm type IV secretion system. The fact that LpnE was exported suggested that the protein may interact with a eukaryotic protein. Using LpnE as bait, we screened a HeLa cell cDNA library for interacting partners, using the yeast two-hybrid system. Examination of the protein-protein interaction between LpnE and a eukaryotic protein, obscurin-like protein 1, suggested that LpnE can interact with eukaryotic proteins containing immunoglobulin-like folds via the SLR regions. This investigation has further characterized the contribution of LpnE to L. pneumophila virulence and, more specifically, the importance of the SLR regions to LpnE function.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia. Phone: (61) 3 8344 8041. Fax: (61) 3 9347 1540. E-mail: hartland{at}unimelb.edu.au

{triangledown} Published ahead of print on 24 September 2007.

Editor: J. L. Flynn

Infection and Immunity, December 2007, p. 5575-5585, Vol. 75, No. 12
0019-9567/07/$08.00+0     doi:10.1128/IAI.00443-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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