This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adriaensen, C.
Right arrow Articles by Hernalsteens, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adriaensen, C.
Right arrow Articles by Hernalsteens, J.-P.

 Previous Article  |  Next Article 

Infection and Immunity, May 2007, p. 2461-2468, Vol. 75, No. 5
0019-9567/07/$08.00+0     doi:10.1128/IAI.01357-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Live Salmonella enterica Serovar Enteritidis Vaccine Allows Serological Differentiation between Vaccinated and Infected Animals{triangledown}

Connie Adriaensen,1 Henri De Greve,1,2 Jean Q. Tian,3 Stéphane De Craeye,1,{dagger} Eline Gubbels,1 Venessa Eeckhaut,4 Filip Van Immerseel,4 Richard Ducatelle,4 Mahesh Kumar,3 and Jean-Pierre Hernalsteens1*

Viral Genetics Laboratory, Faculty of Sciences, Vrije Universiteit Brussel,1 Department of Molecular and Cellular Interactions, Flanders Institute for Biotechnology (VIB), Pleinlaan 2, B-1050 Brussels, Belgium,2 Fort Dodge Animal Health, 800 Fifth Street NW, Fort Dodge, Iowa 50501,3 Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium4

Received 23 August 2006/ Returned for modification 9 October 2006/ Accepted 22 January 2007

Three precisely defined deletion mutants of Salmonella enterica serovar Enteritidis were constructed, a guanine auxotrophic {Delta}guaB mutant, a nonflagellated {Delta}fliC mutant, and an auxotrophic and nonflagellated {Delta}guaB {Delta}fliC double mutant. All three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line T84 and less efficiently internalized in the chicken macrophage cell line HD11. The {Delta}fliC mutant was pathogenic in orally infected BALB/c mice, while the {Delta}guaB mutant was attenuated and conferred protection against a challenge with the pathogenic parent strain. The {Delta}guaB {Delta}fliC double mutant was totally asymptomatic and conferred better protection than the {Delta}guaB mutant. This indicates that the major flagellar protein flagellin is not required for efficient vaccination of BALB/c mice against Salmonella infection. The {Delta}guaB {Delta}fliC mutant was also safe for vaccination of 1-day-old chickens. After two immunizations, it induced statistically significant protection against infection of the internal organs of the birds by a virulent S. enterica serovar Enteritidis challenge strain but not against intestinal colonization. These data demonstrate that nonflagellated attenuated Salmonella mutants can be used as marker vaccines.

* Corresponding author. Mailing address: Viral Genetics Laboratory, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium. Phone: 32 2 6291915. Fax: 32 2 6291912. E-mail: jphernal{at}vub.ac.be

{triangledown} Published ahead of print on 29 January 2007.

Editor: F. C. Fang

{dagger} Present address: Scientific Institute of Public Health, Pasteur Institute, B-1180 Brussels, Belgium.

Infection and Immunity, May 2007, p. 2461-2468, Vol. 75, No. 5
0019-9567/07/$08.00+0     doi:10.1128/IAI.01357-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Saha, S., Takeshita, F., Matsuda, T., Jounai, N., Kobiyama, K., Matsumoto, T., Sasaki, S., Yoshida, A., Xin, K.-Q., Klinman, D. M., Uematsu, S., Ishii, K. J., Akira, S., Okuda, K. (2007). Blocking of the TLR5 Activation Domain Hampers Protective Potential of Flagellin DNA Vaccine. J. Immunol. 179: 1147-1154 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»