This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paixão, T. A.
Right arrow Articles by Santos, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paixão, T. A.
Right arrow Articles by Santos, R. L.

 Previous Article  |  Next Article 

Infection and Immunity, May 2007, p. 2493-2499, Vol. 75, No. 5
0019-9567/07/$08.00+0     doi:10.1128/IAI.01855-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

NRAMP1 3' Untranslated Region Polymorphisms Are Not Associated with Natural Resistance to Brucella abortus in Cattle{triangledown}

Tatiane A. Paixão,1 Fernando P. Poester,2 Alcina V. Carvalho Neta,1 Álan M. Borges,1 Andrey P. Lage,2 and Renato L. Santos1*

Departamento de Clínica e Cirurgia Veterinária,1 Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Minas Gerais, Brazil2

Received 22 November 2006/ Returned for modification 24 January 2007/ Accepted 28 February 2007

The NRAMP1 gene encodes a divalent cation transporter, located in the phagolysosomal membrane of macrophages, that has been associated with resistance to intracellular pathogens. In cattle, natural resistance against brucellosis has been associated with polymorphisms at the 3' untranslated region (3'UTR) of the NRAMP1 gene, which are detectable by single-strand conformational analysis (SSCA). This study aimed to evaluate the association between NRAMP1 3'UTR polymorphisms and resistance against bovine brucellosis in experimental and natural infections. In experimentally infected pregnant cows, abortion occurred in 42.1% of cows with a resistant genotype (SSCAr; n = 19) and in 43.1% of those with a susceptible genotype (SSCAs; n = 23). Furthermore, no association between intensity of pathological changes and genotype was detected. In a farm with a very high prevalence of bovine brucellosis, the percentages of strains of the SSCAr genotype were 86 and 84% in serologically positive (n = 64) and negative (n = 36) cows, respectively. Therefore, no association was found between the NRAMP1-resistant allele and the resistant phenotype in either experimental or naturally occurring brucellosis. To further support these results, bacterial intracellular survival was assessed in bovine monocyte-derived macrophages from cattle with either the resistant or susceptible genotype. In agreement with our previous results, no difference was observed in the rates of intracellular survival of B. abortus within macrophages from cattle with susceptible or resistant genotypes. Taken together, these results indicate that these polymorphisms at the NRAMP1 3'UTR do not affect resistance against B. abortus in cattle and that they are therefore not suitable markers of natural resistance against bovine brucellosis.

* Corresponding author. Mailing address: Escola de Veterinária da UFMG, Depto. Clínica e Cirurgia Vet., Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil. Phone: 55-31-3499-2239. Fax: 55-31-3499-2230. E-mail: rsantos{at}vet.ufmg.br

{triangledown} Published ahead of print on 12 March 2007.

Editor: V. J. DiRita

Infection and Immunity, May 2007, p. 2493-2499, Vol. 75, No. 5
0019-9567/07/$08.00+0     doi:10.1128/IAI.01855-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Anderson, E. S., Paulley, J. T., Gaines, J. M., Valderas, M. W., Martin, D. W., Menscher, E., Brown, T. D., Burns, C. S., Roop, R. M. II (2009). The Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice. Infect. Immun. 77: 3466-3474 [Abstract] [Full Text]  
  • Carvalho Neta, A. V., Stynen, A. P. R., Paixao, T. A., Miranda, K. L., Silva, F. L., Roux, C. M., Tsolis, R. M., Everts, R. E., Lewin, H. A., Adams, L. G., Carvalho, A. F., Lage, A. P., Santos, R. L. (2008). Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus. Infect. Immun. 76: 1897-1907 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»