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Infection and Immunity, June 2007, p. 2946-2953, Vol. 75, No. 6
0019-9567/07/$08.00+0     doi:10.1128/IAI.02011-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evading the Proteasome: Absence of Lysine Residues Contributes to Pertussis Toxin Activity by Evasion of Proteasome Degradation{triangledown}

Zoë E. V. Worthington* and Nicholas H. Carbonetti

Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland

Received 22 December 2006/ Returned for modification 22 February 2007/ Accepted 30 March 2007

Pertussis toxin (PT) is an important virulence factor produced by Bordetella pertussis. PT holotoxin comprises one enzymatically active A subunit (S1), associated with a pentamer of B subunits. PT is an ADP-ribosyltransferase that modifies several mammalian heterotrimeric G proteins. Some bacterial toxins are believed to undergo retrograde intracellular transport through the Golgi apparatus to the endoplasmic reticulum (ER). The ER-associated degradation (ERAD) pathway involves the removal of misfolded proteins from the ER and degradation upon their return to the cytosol; this pathway may be exploited by PT and other toxins. In the cytosol, ERAD substrates are ubiquitinated at lysine residues, targeting them to the proteasome for degradation. We hypothesize that S1 avoids ubiquitination and proteasome degradation due to its lack of lysine residues. We predicted that the addition of lysine residues would reduce PT toxicity by allowing ubiquitination and degradation to occur. Variant forms of PT were engineered, replacing one, two, or three arginines with lysines in a variety of locations on S1. Several variants were identified with wild-type in vitro enzymatic activity but reduced cellular activity, consistent with our hypothesis. Significant recovery of the cellular activity of these variants was observed when CHO cells were pretreated with a proteasome inhibitor. We concluded that the replacement of arginine residues with lysine in the S1 subunit of PT renders the toxin subject to proteasomal degradation, suggesting that wild-type PT avoids proteasome degradation due to an absence of lysine residues.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Maryland School of Medicine, 660 W. Redwood St., HH324, Baltimore, MD 21201. Phone: (410) 706-0097. Fax: (410) 706-2129. E-mail: zphil001{at}umaryland.edu

{triangledown} Published ahead of print on 9 April 2007.

Editor: A. D. O'Brien

Infection and Immunity, June 2007, p. 2946-2953, Vol. 75, No. 6
0019-9567/07/$08.00+0     doi:10.1128/IAI.02011-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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