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Infection and Immunity, August 2007, p. 3969-3978, Vol. 75, No. 8
0019-9567/07/$08.00+0     doi:10.1128/IAI.00157-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of Primary Human Alveolar Epithelial Cells in Host Defense against Francisella tularensis Infection

Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas 77555-1070,¹ Department of Pediatrics, Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas 77555-1436,² Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas 77555-0609³

Received 30 January 2007/ Returned for modification 11 March 2007/ Accepted 4 May 2007

Francisella tularensis, an intracellular pathogen, is highly virulent when inhaled. Alveolar epithelial type I (ATI) and type II (ATII) cells line the majority of the alveolar surface and respond to inhaled pathogenic bacteria via cytokine secretion. We hypothesized that these cells contribute to the lung innate immune response to F. tularensis. Results demonstrated that the live vaccine strain (LVS) contacted ATI and ATII cells by 2 h following intranasal inoculation of mice. In culture, primary human ATI or ATII cells, grown on transwell filters, were stimulated on the apical (AP) surface with virulent F. tularensis Schu 4 or LVS. Basolateral (BL) conditioned medium (CM), collected 6 and 24 h later, was added to the BL surfaces of transwell cultures of primary human pulmonary microvasculature endothelial cells (HPMEC) prior to the addition of polymorphonuclear leukocytes (PMNs) or dendritic cells (DCs) to the AP surface. HPMEC responded to S4- or LVS-stimulated ATII, but not ATI, CM as evidenced by PMN and DC migration. Analysis of the AP and BL ATII CM revealed that both F. tularensis strains induced various levels of a variety of cytokines via NF-B activation. ATII cells pretreated with an NF-B inhibitor prior to F. tularensis stimulation substantially decreased interleukin-8 secretion, which did not occur through Toll-like receptor 2, 2/6, 4, or 5 stimulation. These data indicate a crucial role for ATII cells in the innate immune response to F. tularensis.

Published ahead of print on 14 May 2007.

Editor: J. B. Bliska

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