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Infection and Immunity, September 2007, p. 4597-4607, Vol. 75, No. 9
0019-9567/07/$08.00+0     doi:10.1128/IAI.00630-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Differential Effects of Epinephrine, Norepinephrine, and Indole on Escherichia coli O157:H7 Chemotaxis, Colonization, and Gene Expression{triangledown}

Tarun Bansal, Derek Englert, Jintae Lee, Manjunath Hegde, Thomas K. Wood, and Arul Jayaraman*

Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122

Received 3 May 2007/ Returned for modification 4 June 2007/ Accepted 13 June 2007

During infection in the gastrointestinal tract, enterohemorrhagic Escherichia coli (EHEC) O157:H7 is exposed to a wide range of signaling molecules, including the eukaryotic hormones epinephrine and norepinephrine, and bacterial signal molecules such as indole. Since these signaling molecules have been shown to be involved in the regulation of phenotypes such as motility and virulence that are crucial for EHEC infections, we hypothesized that these molecules also govern the initial recognition of the large intestine environment and attachment to the host cell surface. Here, we report that, compared to indole, epinephrine and norepinephrine exert divergent effects on EHEC chemotaxis, motility, biofilm formation, gene expression, and colonization of HeLa cells. Using a novel two-fluorophore chemotaxis assay, it was found that EHEC is attracted to epinephrine and norepinephrine while it is repelled by indole. In addition, epinephrine and norepinephrine also increased EHEC motility and biofilm formation while indole attenuated these phenotypes. DNA microarray analysis of surface-associated EHEC indicated that epinephrine/norepinephrine up-regulated the expression of genes involved in surface colonization and virulence while exposure to indole decreased their expression. The gene expression data also suggested that autoinducer 2 uptake was repressed upon exposure to epinephrine/norepinephrine but not indole. In vitro adherence experiments confirmed that epinephrine and norepinephrine increased attachment to epithelial cells while indole decreased adherence. Taken together, these results suggest that epinephrine and norepinephrine increase EHEC infection while indole attenuates the process.

* Corresponding author. Mailing address: Artie McFerrin Department of Chemical Engineering, Texas A&M University, 200 Jack E. Brown Engineering, 3122 TAMU, College Station, TX 77843-3122. Phone: (979) 845-3306. Fax: (979) 845-6446. E-mail: arulj{at}tamu.edu

{triangledown} Published ahead of print on 25 June 2007.

Editor: D. L. Burns

Infection and Immunity, September 2007, p. 4597-4607, Vol. 75, No. 9
0019-9567/07/$08.00+0     doi:10.1128/IAI.00630-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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