This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bookwalter, J. E.
Right arrow Articles by Bakaletz, L. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bookwalter, J. E.
Right arrow Articles by Bakaletz, L. O.

 Previous Article  |  Next Article 

Infection and Immunity, January 2008, p. 48-55, Vol. 76, No. 1
0019-9567/08/$08.00+0     doi:10.1128/IAI.00980-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Homologue Plays a Pivotal Role in Nontypeable Haemophilus influenzae Colonization of the Chinchilla Nasopharynx via the Outer Membrane Protein P5-Homologous Adhesin{triangledown}

James E. Bookwalter,1 Joseph A. Jurcisek,1 Scott D. Gray-Owen,2 Soledad Fernandez,3 Glen McGillivary,1 and Lauren O. Bakaletz1*

The Research Institute at Nationwide Children's Hospital and The Ohio State University College of Medicine, Department of Pediatrics, Center for Microbial Pathogenesis, 700 Children's Drive, W591, Columbus, Ohio 43205,1 Department of Medical Genetics and Microbiology, University of Toronto, 1 King's College Circle, Room 4381, Toronto, Ontario M5S 1A8, Canada,2 College of Public Health and Center for Biostatistics, The Ohio State University, 320 W. 10th Avenue, Columbus, Ohio 432103

Received 18 July 2007/ Returned for modification 23 August 2007/ Accepted 3 October 2007

In vitro studies suggest an important role for CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) in infection by multiple gram-negative bacteria. However, in vivo evidence supporting this role is lacking, largely because the bacterial adhesins involved in this host-microbe association do not bind to murine-derived CEACAM1. One of several adhesins expressed by nontypeable Haemophilus influenzae (NTHI), the outer membrane protein P5-homologous adhesin (or P5), is essential for colonization of the chinchilla nasopharynx and infection of the middle ear. Here we reveal that NTHI P5 binds to the chinchilla homologue of CEACAM1 and that rabbit anti-human carcinoembryonic antigen blocks NTHI colonization of the chinchilla nasopharynx, providing the first demonstration of a role for CEACAM receptor binding by any bacterial pathogen in vivo.

* Corresponding author. Mailing address: The Research Institute at Nationwide Children's Hospital, Center for Microbial Pathogenesis, The Ohio State University College of Medicine, Department of Pediatrics, 700 Children's Drive, Rm. W591, Columbus, OH 43205-2696. Phone: (614) 722-2915. Fax: (614) 722-2818. E-mail: Lauren.Bakaletz{at}nationwidechildrens.org

{triangledown} Published ahead of print on 15 October 2007.

Editor: J. N. Weiser

Infection and Immunity, January 2008, p. 48-55, Vol. 76, No. 1
0019-9567/08/$08.00+0     doi:10.1128/IAI.00980-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kisiela, D. I., Czuprynski, C. J. (2009). Identification of Mannheimia haemolytica Adhesins Involved in Binding to Bovine Bronchial Epithelial Cells. Infect. Immun. 77: 446-455 [Abstract] [Full Text]  
  • Rosadini, C. V., Wong, S. M. S., Akerley, B. J. (2008). The Periplasmic Disulfide Oxidoreductase DsbA Contributes to Haemophilus influenzae Pathogenesis. Infect. Immun. 76: 1498-1508 [Abstract] [Full Text]  
  • Novotny, L. A., Partida-Sanchez, S., Munson, R. S. Jr., Bakaletz, L. O. (2008). Differential Uptake and Processing of a Haemophilus influenzae P5-Derived Immunogen by Chinchilla Dendritic Cells. Infect. Immun. 76: 967-977 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»