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Infection and Immunity, November 2008, p. 4924-4933, Vol. 76, No. 11
0019-9567/08/$08.00+0     doi:10.1128/IAI.00531-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Functional Analysis of the Salmonella Pathogenicity Island 2-Mediated Inhibition of Antigen Presentation in Dendritic Cells{triangledown}

Serkan Halici, Sebastian F. Zenk, Jonathan Jantsch, and Michael Hensel*

Mikrobiologisches Institut, Universitätsklinikum Erlangen, Erlangen, Germany

Received 30 April 2008/ Returned for modification 31 May 2008/ Accepted 16 August 2008

Salmonella enterica is a facultative intracellular pathogen that is able to modify host cell functions by means of effector proteins translocated by the type III secretion system (T3SS) encoded by Salmonella Pathogenicity Island 2 (SPI2). The SPI2-T3SS is also active in Salmonella after uptake by murine bone marrow-derived dendritic cells (BM-DC). We have previously shown that intracellular Salmonella interfere with the ability of BM-DC to stimulate antigen-dependent T-cell proliferation in an SPI2-T3SS-dependent manner. We observed that Salmonella-mediated inhibition of antigen presentation could be restored by external addition of peptides on major histocompatibility complex class II (MHC-II). The processing of antigens in Salmonella-infected cells was not altered; however, the intracellular loading of peptides on MHC-II was reduced as a function of the SPI2-T3SS. We set out to identify the effector proteins of the SPI2-T3SS involved in inhibition of antigen presentation and demonstrated that effector proteins SifA, SspH2, SlrP, PipB2, and SopD2 were equally important for the interference with antigen presentation, whereas SseF and SseG contributed to a lesser extent to this phenotype. These observations indicate the presence of a host cell-specific virulence function of a novel subset of SPI2-effector proteins.

* Corresponding author. Mailing address: Mikrobiologisches Institut, Universitätsklinikum Erlangen, Wasserturmstr. 3-5, D-91054 Erlangen, Germany. Phone: 49(0)9131 8523640. Fax: 49(0)9131 8522531. E-mail: hensel{at}mikrobio.med.uni-erlangen.de

{triangledown} Published ahead of print on 2 September 2008.

Editor: A. J. Bäumler

Infection and Immunity, November 2008, p. 4924-4933, Vol. 76, No. 11
0019-9567/08/$08.00+0     doi:10.1128/IAI.00531-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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