This Article Full Text Full Text (PDF) Other Versions of this Article: IAI.00444-07v1 76/2/767    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Cai, X. Articles by Lu, C. Search for Related Content PubMed PubMed Citation Articles by Cai, X. Articles by Lu, C.

 Previous Article  |  Next Article 

Infection and Immunity, February 2008, p. 767-770, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.00444-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effective Production and Purification of the Glycosylated TSOL18 Antigen, Which Is Protective against Pig Cysticercosis

Xuepeng Cai,^1,* Gailing Yuan,^1,* Yadong Zheng,^1, Xuenong Luo,¹ Shaohua Zhang,¹ Juntao Ding,¹ Zhizhong Jing,¹ and Chengping Lu²

Key Laboratory of Zoonoses of CAAS, Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, Gansu,¹ Nanjing Agricultural University, Weigang 210095, Nanjing, China²

Received 27 March 2007/ Returned for modification 9 May 2007/ Accepted 16 November 2007

Cysticercosis caused by Taenia solium metacestodes is a worldwide public health problem. Important progress in the development of effective and practical vaccines against this disease has been made. In this study, the promising T. solium oncospheral vaccine candidate named TSOL18 antigen was produced in a 5-liter fermentor. During the process of fermentation, the pH of the culture was always kept below 5.0, and in order to prevent foaming, an antifoam agent was added. In addition, the oxygen content of the culture was constantly kept at >50% in our experiment. A high level of the glycosylated protein (2.5 g/liter) was obtained, and the protein was easily purified by gel chromatography. Vaccination trials showed that the recombinant TSOL18 antigen induced 94 and 100% reductions in metacestode burdens in vaccinated pigs, obviously higher than the 89% reduction in pigs immunized with cysticercus crude extracts in trial 1. These are very promising results in the development of an efficient tool to control cysticercosis in Asia.

Published ahead of print on 10 December 2007.

Editor: W. A. Petri, Jr.

These authors equally contributed to this work.