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Infection and Immunity, July 2008, p. 2833-2842, Vol. 76, No. 7
0019-9567/08/$08.00+0     doi:10.1128/IAI.00043-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Francisella tularensis Invasion of Lung Epithelial Cells{triangledown}

Robin R. Craven, Joshua D. Hall, James R. Fuller, Sharon Taft-Benz, and Thomas H. Kawula*

Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Received 11 January 2008/ Returned for modification 19 February 2008/ Accepted 11 April 2008

Francisella tularensis, a gram-negative facultative intracellular bacterial pathogen, causes disseminating infections in humans and other mammalian hosts. Macrophages and other monocytes have long been considered the primary site of F. tularensis replication in infected animals. However, recently it was reported that F. tularensis also invades and replicates within alveolar epithelial cells following inhalation in a mouse model of tularemia. TC-1 cells, a mouse lung epithelial cell line, were used to study the process of F. tularensis invasion and intracellular trafficking within nonphagocytic cells. Live and paraformaldehyde-fixed F. tularensis live vaccine strain organisms associated with, and were internalized by, TC-1 cells at similar frequencies and with indistinguishable differences in kinetics. Inhibitors of microfilament and microtubule activity resulted in significantly decreased F. tularensis invasion, as did inhibitors of phosphatidylinositol 3-kinase and tyrosine kinase activity. Collectively, these results suggest that F. tularensis epithelial cell invasion is mediated by a preformed ligand on the bacterial surface and driven entirely by host cell processes. Once internalized, F. tularensis-containing endosomes associated with early endosome antigen 1 (EEA1) followed by lysosome-associated membrane protein 1 (LAMP-1), with peak coassociation frequencies occurring at 30 and 120 min postinoculation, respectively. By 2 h postinoculation, 70.0% (± 5.5%) of intracellular bacteria were accessible to antibody delivered to the cytoplasm, indicating vacuolar breakdown and escape into the cytoplasm.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, CB# 7290, 804 MEJB, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7290. Phone: (919) 966-9699. Fax: (919) 962-8103. E-mail: kawula{at}med.unc.edu

{triangledown} Published ahead of print on 21 April 2008.

Editor: J. N. Weiser

Infection and Immunity, July 2008, p. 2833-2842, Vol. 76, No. 7
0019-9567/08/$08.00+0     doi:10.1128/IAI.00043-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schulert, G. S., McCaffrey, R. L., Buchan, B. W., Lindemann, S. R., Hollenback, C., Jones, B. D., Allen, L.-A. H. (2009). Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS. Infect. Immun. 77: 1324-1336 [Abstract] [Full Text]  
  • Fuller, J. R., Craven, R. R., Hall, J. D., Kijek, T. M., Taft-Benz, S., Kawula, T. H. (2008). RipA, a Cytoplasmic Membrane Protein Conserved among Francisella Species, Is Required for Intracellular Survival. Infect. Immun. 76: 4934-4943 [Abstract] [Full Text]  


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