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Infection and Immunity, January 2009, p. 446-455, Vol. 77, No. 1
0019-9567/09/$08.00+0     doi:10.1128/IAI.00312-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Mannheimia haemolytica Adhesins Involved in Binding to Bovine Bronchial Epithelial Cells{triangledown}

Dagmara I. Kisiela and Charles J. Czuprynski*

Department of Pathobiological Sciences, University of Wisconsin-Madison, School of Veterinary Medicine, Madison, Wisconsin

Received 9 March 2008/ Returned for modification 18 April 2008/ Accepted 27 October 2008

Mannheimia haemolytica, a commensal organism of the upper respiratory tract in cattle, is the principal bacterial pathogen associated with the bovine respiratory disease complex. Adherence to the respiratory mucosa is a crucial event in its pathogenesis. However, the bacterial components that contribute to this process are not fully characterized. In this study, we demonstrated that M. haemolytica adhered to bovine bronchial epithelial cells (BBEC) in vitro and that adherence was inhibited by anti-M. haemolytica antibody. Western blot analysis of M. haemolytica proteins that bind to BBEC showed a dominant protein band with an apparent molecular mass of ~30 kDa. Peptide sequences for the 30-kDa BBEC-binding proteins, as determined by liquid chromatography-tandem mass spectrometry, matched two M. haemolytica surface proteins: heat-modifiable outer membrane protein A (OmpA) and lipoprotein 1 (Lpp1). Western blotting showed that the 30-kDa protein band is recognized by both anti-M. haemolytica OmpA and anti-Lpp1 antibodies. Furthermore, incubation with anti-OmpA and anti-Lpp1 antibodies significantly inhibited M. haemolytica binding to BBEC monolayers. In summary, these results suggest that OmpA and Lpp1 contribute to adherence of M. haemolytica to bovine respiratory epithelial cells.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706. Phone and fax: 608 262 8102. E-mail: czuprync{at}svm.vetmed.wisc.edu

{triangledown} Published ahead of print on 3 November 2008.

Editor: J. F. Urban, Jr.

Infection and Immunity, January 2009, p. 446-455, Vol. 77, No. 1
0019-9567/09/$08.00+0     doi:10.1128/IAI.00312-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gaddy, J. A., Tomaras, A. P., Actis, L. A. (2009). The Acinetobacter baumannii 19606 OmpA Protein Plays a Role in Biofilm Formation on Abiotic Surfaces and in the Interaction of This Pathogen with Eukaryotic Cells. Infect. Immun. 77: 3150-3160 [Abstract] [Full Text]  


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