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Infection and Immunity, January 2009, p. 456-463, Vol. 77, No. 1
0019-9567/09/$08.00+0     doi:10.1128/IAI.00503-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Human Polymorphonuclear Neutrophil Responses to Burkholderia pseudomallei in Healthy and Diabetic Subjects{triangledown}

Sujin Chanchamroen,1 Chidchamai Kewcharoenwong,1 Wattanachai Susaengrat,2 Manabu Ato,3 and Ganjana Lertmemongkolchai1*

The Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University,1 Department of Medicine, Khon Kaen Hospital, Ministry of Public Health, Khon Kaen, Thailand,2 Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan3

Received 23 April 2008/ Returned for modification 29 May 2008/ Accepted 26 September 2008

The major predisposing factor for melioidosis is diabetes mellitus, but no immunological mechanisms have been investigated to explain this. In this study, polymorphonuclear neutrophil (PMN) responses to Burkholderia pseudomallei, the causative agent of melioidosis, in healthy and diabetic Thai subjects were determined by flow cytometry. The results showed that B. pseudomallei displayed reduced uptake by PMNs compared to Salmonella enterica serovar Typhimurium and Escherichia coli. Additionally, intracellular survival of B. pseudomallei was detected throughout a 24-h period, indicating the intrinsic resistance of B. pseudomallei to killing by PMNs. Moreover, PMNs from diabetic subjects displayed impaired phagocytosis of B. pseudomallei, reduced migration in response to interleukin-8, and an inability to delay apoptosis. These data show that B. pseudomallei is intrinsically resistant to phagocytosis and killing by PMNs. These observations, together with the impaired migration and apoptosis in diabetes mellitus, may explain host susceptibility in melioidosis.

* Corresponding author. Mailing address: Center for Research and Development in Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. Phone: 66 4320 3825. Fax: 66 4320 3826. E-mail: ganja_le{at}kku.ac.th

{triangledown} Published ahead of print on 27 October 2008.

Editor: B. A. McCormick

Infection and Immunity, January 2009, p. 456-463, Vol. 77, No. 1
0019-9567/09/$08.00+0     doi:10.1128/IAI.00503-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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