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Infection and Immunity, April 2009, p. 1383-1388, Vol. 77, No. 4
0019-9567/09/$08.00+0     doi:10.1128/IAI.01156-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Direct Regulation by the Vibrio cholerae Regulator ToxT To Modulate Colonization and Anticolonization Pilus Expression{triangledown}

Ansel Hsiao ,1,{dagger},{ddagger} Xiao Xu,2,{dagger} Biao Kan,2 Rahul V. Kulkarni,3 and Jun Zhu1,4*

Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104,1 The State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing, China,2 Department of Physics, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,3 The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China4

Received 16 September 2008/ Returned for modification 21 October 2008/ Accepted 12 January 2009

The pathogen Vibrio cholerae uses a large number of coordinated transcriptional regulatory events to transition from its environmental reservoir to the host and establish itself at its preferred colonization site at the host intestinal mucosa. The key regulator in this process is the AraC/XylS family transcription factor, ToxT, which plays critical roles in pathogenesis, including the regulation of two type IV pili, the anticolonization factor mannose-sensitive hemagglutinin and the toxin-coregulated pilus. Previously, it was thought ToxT required dimerization in order to effect transcriptional regulation at its cognate promoters. Here, we present evidence that ToxT directly represses transcription of the msh operon by binding to three promoters within this operon and that dimerization may not be required for transcriptional repression of target promoters by ToxT, suggesting that this regulator uses different mechanisms to modulate the transcriptional repertoire of V. cholerae.

* Corresponding author. Mailing address: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104. Phone: (215) 573-4104. Fax: (215) 898-9557. E-mail: junzhu{at}mail.med.upenn.edu

{triangledown} Published ahead of print on 21 January 2009.

Editor: A. Camilli

{dagger} A.H. and X.X. contributed equally to this study.

{ddagger} Present address: Center for Genome Sciences, Washington University, St. Louis, MO 63108.

Infection and Immunity, April 2009, p. 1383-1388, Vol. 77, No. 4
0019-9567/09/$08.00+0     doi:10.1128/IAI.01156-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Glenn, G. M., Francis, D. H., Danielsen, E. M. (2009). Toxin-Mediated Effects on the Innate Mucosal Defenses: Implications for Enteric Vaccines. Infect. Immun. 77: 5206-5215 [Abstract] [Full Text]  


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