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Infect. Immun. doi:10.1128/IAI.00119-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification of commensal bacterial strains modulating Yersinia and DSS-induced inflammatory responses: implications for the development of probiotics

Institute of Medical Microbiology and Hygiene, University of Tübingen, Germany

^* To whom correspondence should be addressed. Email: julia-stefanie.frick{at}med.uni-tuebingen.de.

	Abstract

An increasing body of evidence suggests that probiotic bacteria are effective in the treatment of enteric infections although the molecular basis of this activity remains elusive. To identify putative probiotics we tested commensal bacteria in terms of toxicity, invasiveness, inhibition of Yersinia induced inflammation in vitro and in vivo and modulation of dextran-sodium-sulfate (DSS) induced colitis in mice. Commensal E. coli, Bifidobacterium adolescentis, Bacteroides vulgatus, Bacteroides distasonis and Streptococcus salivarius were screened for adhesion to, invasion into as well as toxicity for host epithelial cells (EC) and the strains were tested for their ability to inhibit Y. enterocolitica induced NF-B activation. Additionally B. adolescentis was administered to mice, orally infected with Y. enterocolitica and to mice with a mucosa impaired by DSS treatment. None of the commensal bacteria tested was toxic for or invaded into EC. B. adolescentis, B. distasonis, B. vulgatus and S. salivarius inhibited Y. enterocolitica induced NF-B activation and IL-8 production in EC. In line with these findings B. adolescentis fed mice had significantly lower mean pathogen burden in visceral organs, intestinal TNF- mRNA expression and loss of bodyweight upon oral infection with Y. enterocolitica. In addition, administration of B. adolescentis decelerated inflammation upon DSS treatment in mice. We suggest that our approach might help to identify new probiotics to be used for treatment of inflammatory and infectious gastrointestinal disorders.

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