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Infect. Immun. doi:10.1128/IAI.00372-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Lipase 8 affects the pathogenesis of Candida albicans

Attila Gácser*, Frank Stehr, Cathrin Kröger, László Kredics, Wilhelm Schäfer, and Joshua D. Nosanchuk

Departments of Medicine (Division of Infectious Diseases) and Microbiology/Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Center of Applied Molecular Biology, University of Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany; Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

* To whom correspondence should be addressed. Email: gacsera{at}gmail.com.


  Abstract

The production of lipases can affect microbial fitness and virulence. We have examined the role of the lipase 8 (LIP8) gene in the virulence of C. albicans by constructing {Delta} lip8 strains by the URA-blaster disruption method. RT-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and over-expression mutants were generated by introducing a LIP8-ORF under the control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥ 7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.




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