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Infect. Immun. doi:10.1128/IAI.00722-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Role of lgtC in Resistance to Human Serum of Nontypeable Haemophilus influenzae Strain R2866

Microbial Pathogens Program, Seattle Biomedical Research Institute, Seattle, Washington 98109; Chemistry Core, Buck Institute, 8001 Redwood Blvd. Novato, California 94945; Department of Pathobiology, University of Washington, Seattle, Washington 98195; Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, Missouri; Department of Microbiology, University of Iowa, Iowa City, Iowa, 52242

^* To whom correspondence should be addressed. Email: arnold.smith{at}sbri.org.

	Abstract

We are investigating a nontypeable Haemophilus influenzae (NTHi) strain, R2866, isolated from a child with meningitis. R2866 is unusually resistant to killing by normal human serum. The serum IC₅₀ for this strain is 18%, approaching that of encapsulated H. influenzae. R3392 is a derivative of R2866 that was found to have increased sensitivity to human serum (IC₅₀ 1.5%). Analysis of tetrameric repeat regions within lipooligosaccharide (LOS) biosynthetic genes in both strains indicated that the glycosyltransferase gene lgtC was OFF (out of frame) in most colonies of R3392, but ON in most colonies of the parent. We sought antigenic and biochemical evidence for modification of the LOS structure. In a whole-cell ELISA, strain R3392 displayed reduced binding of the Gal1,4Gal-specific monoclonal antibody 4C4. Mass spectrometry analysis of LOS from strain R2866 indicated that the primary oligosaccharide glycoform contained four heptose and four hexose residues, while that of R3392 contained four heptose and three hexose residues. We conclude that the R2866 lgtC gene encodes a galactosyltransferase involved in synthesis of the 4C4 epitope, as in other strains, and that expression of lgtC is associated with the high-level serum resistance that has been observed for this strain. This is the first description of the genetic basis of high-level serum resistance in NTHi, as well as the first description of LOS composition in an NTHi strain for which the complete genome sequence has been determined.

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