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Infect. Immun. doi:10.1128/IAI.00833-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

C57BL/6 and congenic interleukin-10 deficient mice can serve as models of Campylobacter jejuni colonization and enteritis

National Food Safety and Toxicology Center, Department of Microbiology and Molecular Genetics, Department of Large Animal Clinical Sciences, Department of Food Science and Human Nutrition, Department of Internal Medicine/Infectious Diseases Division, Michigan State University, East Lansing, Michigan, USA, Department of Parasitology, College of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

^* To whom correspondence should be addressed. Email: mansfie4{at}cvm.msu.edu,

	Abstract

Campylobacter jejuni is a globally distributed cause of human food borne enteritis and has been linked to chronic joint and neurological diseases. We hypothesized that C. jejuni 11168 colonizes the gastrointestinal tract of both C57BL/6 mice and congenic C57BL/6 IL-10 -/- mice and that C57BL/6 IL-10 -/- mice experience C. jejuni 11168 mediated clinical signs and pathology. Individually housed mice were challenged orally with C. jejuni 11168 and the course of infection monitored by clinical examination, bacterial culture, C. jejuni-specific PCR, gross- and histopathology, immunohistochemistry, and anti-C. jejuni specific serology. Ceca of C. jejuni 11168 infected mice were colonized at high rates: 50/50 wild type mice and 168/170 IL-10 -/- mice. In a range from 2-35 days after infection with C. jejuni 11168, C57BL/6 IL-10 -/- mice developed severe typhlocolitis best evaluated at the ileocecocolic junction. Rates of colonization and enteritis did not differ between male and female mice. A dose response experiment showed that as little as 10⁶ cfu produced significant disease and pathological lesions similar to responses seen in humans. Immunohistochemical staining demonstrated C. jejuni antigens within gastrointestinal tissues of infected mice. Significant anti-C. jejuni plasma immunoglobulin levels developed by day 28 after infection in both wild type and IL-10-deficient animals; antibodies were predominantly T helper cell 1 (Th1) associated subtypes. These results indicate that colonization of the mouse gastrointestinal tract by C. jejuni 11168 is necessary but not sufficient for development of enteritis and that C57BL/6 IL-10-/- mice can serve as models for the study of C. jejuni enteritis in humans.

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