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Infect. Immun. doi:10.1128/IAI.00948-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Protective Effect of the Nramp1 BB Genotype against Brucella abortus in Water Buffalo (Bubalus bubalis)

Faculty of Biotechnological Sciences, University of Naples "Federico II", Naples, Italy; Istituto Zooprofilattico Sperimentale per il Mezzogiorno, Portici, Italy; Chair of Immunology, Faculty of Agriculture, University of Naples "Federico II", Naples, Italy; Regione Campania, Assessorato all'Agricoltura, Naples, Italy; and Faculty of Veterinary Medicine, University of Naples "Federico II", Naples, Italy

^* To whom correspondence should be addressed. Email: iannelli{at}unina.it.

	Abstract

We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti Brucella abortus antibodies (by the skin test, agglutination test and complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (A, B, C, D) were detected in the 3' untranslated region of the Nramp1 gene. The BB genotype was represented only among controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria when compared with the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

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