This Article Full Text (PDF) Other Versions of this Article: IAI.01262-06v1 75/3/1129    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Broekhuizen, C. A.N. Articles by Zaat, S. A.J. Search for Related Content PubMed PubMed Citation Articles by Broekhuizen, C. A.N. Articles by Zaat, S. A.J.

 Previous Article  |  Next Article 

Infect. Immun. doi:10.1128/IAI.01262-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Peri-implant tissue is an important niche for Staphylococcus epidermidis in experimental biomaterial-associated infection in mice

Department of Medical Microbiology, CINIMA (Center for Infection and Immunity Amsterdam), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands; Emergent Europe, 540-545 Eskdale Road, Winnersh Triangle, Wokingham, RG41 5TU, Berkshire, UK; Department of Pathology, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; Department of Medical Microbiology and Infectious Diseases, VU Medical Center, Amsterdam, The Netherlands

^* To whom correspondence should be addressed. Email: s.a.zaat{at}amc.uva.nl.

	Abstract

Biomaterial-associated infections (BAI), predominantly caused by Staphylococcus epidermidis, are a significant problem in modern medicine. Biofilm formation is considered the pivotal element in the pathogenesis, but in previous mouse studies we retrieved S. epidermidis from peri-implant tissue. To assess the kinetics and generality of tissue colonization, we investigated BAI using two S. epidermidis strains, two biomaterials, and two mouse strains. At low inocula all implants were culture negative whereas surrounding tissues were positive. At higher doses tissues were more often culture positive than implants, with higher numbers of CFU. This was true for the different biomaterials tested, for both S. epidermidis strains, at different time points, and for both mouse strains. S. epidermidis co-localized with host cells at a distance of >10 cell layers from the biomaterial-tissue interface. We conclude that in mouse experimental BAI S. epidermidis peri-implant tissue colonization is more important than biofim formation.

This article has been cited by other articles:

• Nejadnik, M. R., Engelsman, A. F., Saldarriaga Fernandez, I. C., Busscher, H. J., Norde, W., van der Mei, H. C. (2008). Bacterial colonization of polymer brush-coated and pristine silicone rubber implanted in infected pockets in mice. J Antimicrob Chemother 0: dkn395v1-3 [Abstract] [Full Text]