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Infect. Immun. doi:10.1128/IAI.01429-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The wbt locus of Francisella tularensis: role in LPS O antigen biogenesis and pathogenicity

Catherine Raynaud, Karin L. Meibom, Marie-Annick Lety, Iharilalao Dubail, Thomas Candela, Eric Frapy, and Alain Charbit*

Université Paris Descartes, Faculté de Médecine René Descartes, Paris, F-75015 France; Inserm, U570, Unité de Pathogénie des Infections Systémiques, Paris, F-75015, France; Unité Toxines et pathogénie bactérienne CNRS-URA2172, 28, rue de Dr Roux, 75724 Paris Cedex 15 - France

* To whom correspondence should be addressed. Email: charbit{at}necker.fr.


  Abstract

Francisella tularensis is a highly infectious bacterial pathogen, responsible for the zoonotic disease tularemia. We screened a bank of transposon insertion mutants of F. tularensis subspecies horlarctica LVS for colony morphology alterations and selected a mutant with a transposon insertion in wbtA, the first gene of the predicted LPS O antigen gene cluster. Inactivation of wbtA led to the complete loss of O antigen, conferred serum sensitivity, impaired intracellular replication and severely attenuated virulence in the mouse model. Notably, this mutant afforded protection against a challenge against virulent LVS.


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