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Infect. Immun. doi:10.1128/IAI.01447-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The Rab6 effector, BICD1, associates with Chlamydia trachomatis inclusions in a biovar-specific manner

Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853

^* To whom correspondence should be addressed. Email: ms299{at}cornell.edu.

	Abstract

Chlamydia species are obligate intracellular bacteria that replicate within a membrane-bound vacuole, the inclusion, which is trafficked to the peri-Golgi region by processes that are dependent on early chlamydial gene expression. Although neither the host nor the chlamydial proteins that regulate the intracellular trafficking have been clearly defined, several enhanced green fluorescent protein (EGFP)-tagged Rab GTPases, including Rab6, are recruited to Chlamydia trachomatis inclusions. To further characterize the association of Rab6 with the C. trachomatis inclusion, we examined the intracellular localization of guanine-nucleotide binding mutants of Rab6 and demonstrated that only active GTP-bound, and not inactive GDP-bound EGFP-Rab6 mutants were recruited to the inclusion, suggesting that EGFP-Rab6 interacts with the inclusion via a host Rab6 effector or a chlamydial protein that mimics a Rab6 effector. Using EGFP-tagged fusion proteins, we also demonstrated that the Rab6 effector, Bicaudal-D1 (BICD1), localized to C. trachomatis inclusions in a biovar-specific manner. In addition, we demonstrated that EGFP-Rab6 and its effector, EGFP-BICD1, are recruited to the inclusion in a microtubule- and Golgi-independent but chlamydial gene expression-dependent mechanism. Finally, in contrast to the Rab6-dependent Golgi localization of endogenous BICD1, EGFP-BICD1 was recruited to the inclusion by a Rab6-independent mechanism. Collectively, these data demonstrate that neither Rab6 nor BICD1 is trafficked to the inclusion via a Golgi-localized intermediate suggesting that each protein is trafficked to the C. trachomatis serovar L2 inclusion by a unique, but as yet undefined, mechanism.

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