This Article Full Text (PDF) Other Versions of this Article: IAI.01529-06v1 75/2/878    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Philipovskiy, A. V. Articles by Smiley, S. T. Search for Related Content PubMed PubMed Citation Articles by Philipovskiy, A. V. Articles by Smiley, S. T.

 Previous Article  |  Next Article 

Infect. Immun. doi:10.1128/IAI.01529-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Vaccination with live Yersinia pestis primes CD4 and CD8 T cells that synergistically protect against lethal pulmonary Y. pestis infection

Trudeau Institute, Saranac Lake, NY 12983

^* To whom correspondence should be addressed. Email: ssmiley{at}trudeauinstitute.org.

	Abstract

Vaccination with live attenuated Yersinia pestis confers protection against pneumonic plague but is not considered safe for general use. Subunit plague vaccines containing the Y. pestis F1 and LcrV proteins prime robust antibody responses but may not provide sufficient protection. To aid the development of a safe and effective plague vaccine, we are investigating roles for T cells during defense against Y. pestis infection. Here we demonstrate that vaccination of mice with live Y. pestis primes specific CD4 and CD8 T cells that, upon purification and direct transfer to naïve mice, synergistically protect against lethal intranasal Y. pestis challenge. While not preventing extrapulmonary dissemination, the co-administered T cells promote bacterial clearance and reduce bacteremia. These observations strongly suggest that pneumonic plague vaccines should strive to prime both CD4 and CD8 T cells. Finally, we demonstrate that vaccination with live Y. pestis primes CD4 and CD8 T cells that respond to Y. pestis strains lacking the capacity to express F1, LcrV and all pCD1/pPCP-encoded proteins, suggesting that protective T cells likely recognize antigens distinct from those previously defined as targets for humoral immunity.

This article has been cited by other articles:

• Blisnick, T., Ave, P., Huerre, M., Carniel, E., Demeure, C. E. (2008). Oral Vaccination against Bubonic Plague Using a Live Avirulent Yersinia pseudotuberculosis Strain. Infect. Immun. 76: 3808-3816 [Abstract] [Full Text]  
• Li, B., Yang, R. (2008). Interaction between Yersinia pestis and the Host Immune System. Infect. Immun. 76: 1804-1811 [Full Text]  
• Sha, J., Agar, S. L., Baze, W. B., Olano, J. P., Fadl, A. A., Erova, T. E., Wang, S., Foltz, S. M., Suarez, G., Motin, V. L., Chauhan, S., Klimpel, G. R., Peterson, J. W., Chopra, A. K. (2008). Braun Lipoprotein (Lpp) Contributes to Virulence of Yersiniae: Potential Role of Lpp in Inducing Bubonic and Pneumonic Plague. Infect. Immun. 76: 1390-1409 [Abstract] [Full Text]