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Research Article

Reactivity of type-specific monoclonal antibodies with Staphylococcus aureus clinical isolates and purified capsular polysaccharide.

M J Nelles, C A Niswander, W W Karakawa, W F Vann, R D Arbeit
M J Nelles
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C A Niswander
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W W Karakawa
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W F Vann
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R D Arbeit
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ABSTRACT

Staphylococcus aureus has been classified into at least eight different capsular types by using polyclonal rabbit antisera specific for their associated capsular polysaccharides. We produced and characterized monoclonal antibodies reactive with two serologically distinct capsular types, types 5 and 8, which account for more than 70% of all S. aureus bacteremias. These type-specific, monoclonal antibodies reacted with S. aureus clinical isolates possessing the homologous capsular type and exhibited no cross-reactivity against S. aureus clinical isolates possessing the heterologous capsular type, nontypeable S. aureus clinical isolates, Staphylococcus epidermidis clinical isolates, or a variety of gram-negative organisms. The anti-type 8 monoclonal antibodies also reacted with purified capsular polysaccharide derived from the prototype type 8 S. aureus strain.

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Reactivity of type-specific monoclonal antibodies with Staphylococcus aureus clinical isolates and purified capsular polysaccharide.
M J Nelles, C A Niswander, W W Karakawa, W F Vann, R D Arbeit
Infection and Immunity Jul 1985, 49 (1) 14-18; DOI:

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Reactivity of type-specific monoclonal antibodies with Staphylococcus aureus clinical isolates and purified capsular polysaccharide.
M J Nelles, C A Niswander, W W Karakawa, W F Vann, R D Arbeit
Infection and Immunity Jul 1985, 49 (1) 14-18; DOI:
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