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Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, Non-P.H.S. | Research Support, U.S. Gov't, P.H.S.

Helicobacter pylori cytotoxin induces vacuolation of primary human mucosal epithelial cells.

P R Harris, T L Cover, D R Crowe, J M Orenstein, M F Graham, M J Blaser, P D Smith
P R Harris
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, USA.
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T L Cover
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, USA.
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D R Crowe
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, USA.
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J M Orenstein
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, USA.
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M F Graham
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, USA.
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M J Blaser
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, USA.
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P D Smith
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, USA.
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ABSTRACT

We investigated whether Helicobacter pylori cytotoxin induces vacuolation in primary epithelial cells from normal human mucosa. Epithelial cells purified by enzyme digestion and elutriation were evaluated for vacuolation in a blinded protocol by light and electron microscopy before and after incubation with culture supernatant (CS) from H. pylori 60190, which has vacuolating activity for HeLa cells (Tox+), and isogenic H. pylori mutant 60190-v1, which lacks this activity (Tox-). Primary epithelial cells (>98% pure) exposed to CS from Tox+ H. pylori exhibited marked vacuolation (52% +/- 5% of cells) compared with epithelial cells exposed to either CS from Tox- H. pylori (23% +/- 3.2%) or uninoculated control broth (23% +/- 3.7%) (P < 0.05) by light microscopy, which was confirmed by electron microscopy and antibody inhibition studies. These are the first data to show that H. pylori cytotoxin causes vacuolation of primary human mucosal epithelial cells.

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Helicobacter pylori cytotoxin induces vacuolation of primary human mucosal epithelial cells.
P R Harris, T L Cover, D R Crowe, J M Orenstein, M F Graham, M J Blaser, P D Smith
Infection and Immunity Nov 1996, 64 (11) 4867-4871; DOI:

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Helicobacter pylori cytotoxin induces vacuolation of primary human mucosal epithelial cells.
P R Harris, T L Cover, D R Crowe, J M Orenstein, M F Graham, M J Blaser, P D Smith
Infection and Immunity Nov 1996, 64 (11) 4867-4871; DOI:
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