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Journal Article | Research Support, Non-U.S. Gov't

A new fimbrial antigen harbored by CAZ-5/SHV-4-producing Klebsiella pneumoniae strains involved in nosocomial infections.

P Di Martino, V Livrelli, D Sirot, B Joly, A Darfeuille-Michaud
P Di Martino
Laboratoire de Bactériologie, Facultés de Pharmacie et Médecine, Clermont-Ferrand, France.
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V Livrelli
Laboratoire de Bactériologie, Facultés de Pharmacie et Médecine, Clermont-Ferrand, France.
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D Sirot
Laboratoire de Bactériologie, Facultés de Pharmacie et Médecine, Clermont-Ferrand, France.
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B Joly
Laboratoire de Bactériologie, Facultés de Pharmacie et Médecine, Clermont-Ferrand, France.
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A Darfeuille-Michaud
Laboratoire de Bactériologie, Facultés de Pharmacie et Médecine, Clermont-Ferrand, France.
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ABSTRACT

We purified and characterized a new fimbria termed KPF-28 (Klebsiella pneumoniae fimbria with a fimbrin molecular mass of 28 kDa) involved in K. pneumoniae adherence to the human carcinoma cell line Caco-2. Electron microscopy of bacterial surface protein preparations and immunogold labeling of bacterial cells showed that KPF-28 was a long, thin, and flexible fimbria about 4 to 5 nm in diameter and 0.5 to 2 microm long. The N-terminal amino acid sequence of the KPF-28 major fimbrial subunit showed no homology with type 1 and type 3 pili of K. pneumoniae but showed 61.7% identity with residues 6 to 19 of the N-terminal amino acid sequence of PapA, the Pap major pilus subunit expressed by uropathogenic Escherichia coli strains. Total amino acid content determination showed that the KPF-28 major subunit composition was close to that of the GVVPQ fimbrial family major subunits expressed by pathogenic E. coli strains. The study of the prevalence of KPF-28 among K. pneumoniae strains involved in nosocomial infections revealed that KPF-28 was found in the great majority of the K. pneumoniae strains producing the CAZ-5/SHV-4 extended-spectrum beta-lactamase. As shown by curing and mating experiments, the R plasmid encoding the CAZ-5/SHV-4 enzyme was found to be involved in but not solely responsible for KPF-28 expression. Hybridization experiments using an oligonucleotide probe corresponding to the N-terminal part of the 28-kDa protein revealed that the structural gene encoding the KPF-28 major subunit was localized on this R plasmid. KPF-28 is a putative colonization factor of the human gut, since the ceftazidine-sensitive derivative strain CF914-1C no longer adhered and since the Fab fragments of antibodies raised against KPF-28 inhibited adhesion of K. pneumoniae CF914-1 to the Caco-2 cell line.

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A new fimbrial antigen harbored by CAZ-5/SHV-4-producing Klebsiella pneumoniae strains involved in nosocomial infections.
P Di Martino, V Livrelli, D Sirot, B Joly, A Darfeuille-Michaud
Infection and Immunity Jun 1996, 64 (6) 2266-2273; DOI:

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A new fimbrial antigen harbored by CAZ-5/SHV-4-producing Klebsiella pneumoniae strains involved in nosocomial infections.
P Di Martino, V Livrelli, D Sirot, B Joly, A Darfeuille-Michaud
Infection and Immunity Jun 1996, 64 (6) 2266-2273; DOI:
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