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Journal Article

Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus.

J A Calera, S Paris, M Monod, A J Hamilton, J P Debeaupuis, M Diaquin, R López-Medrano, F Leal, J P Latgé
J A Calera
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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S Paris
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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M Monod
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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A J Hamilton
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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J P Debeaupuis
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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M Diaquin
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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R López-Medrano
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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F Leal
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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J P Latgé
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
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ABSTRACT

Aspergillus fumigatus possesses two catalases (described as fast and slow on the basis of their electrophoretic mobility). The slow catalase has been recognized as a diagnostic antigen for aspergillosis in immunocompetent patients. The antigenic catalase has been purified. The enzyme is a tetrameric protein composed of 90-kDa subunits. The corresponding cat1 gene was cloned, and sequencing data show that the cat1 gene codes for a 728-amino-acid polypeptide. A recombinant protein expressed in Pichia pastoris is enzymatically active and has biochemical and antigenic properties that are similar to those of the wild-type catalase. Molecular experiments reveal that CAT1 contains a signal peptide and a propeptide of 15 and 12 amino acid residues, respectively. cat1-disrupted mutants that were unable to produce the slow catalase were as sensitive to H2O2 and polymorphonuclear cells as the wild-type strain. In addition, there was no difference in pathogenicity between the cat1 mutant and its parental cat1+ strain in a murine model of aspergillosis.

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Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus.
J A Calera, S Paris, M Monod, A J Hamilton, J P Debeaupuis, M Diaquin, R López-Medrano, F Leal, J P Latgé
Infection and Immunity Nov 1997, 65 (11) 4718-4724; DOI:

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Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus.
J A Calera, S Paris, M Monod, A J Hamilton, J P Debeaupuis, M Diaquin, R López-Medrano, F Leal, J P Latgé
Infection and Immunity Nov 1997, 65 (11) 4718-4724; DOI:
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