Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Infection and Immunity
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Clinical Trial | Journal Article | Randomized Controlled Trial | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.

Safety of live oral Salmonella typhi vaccine strains with deletions in htrA and aroC aroD and immune response in humans.

C O Tacket, M B Sztein, G A Losonsky, S S Wasserman, J P Nataro, R Edelman, D Pickard, G Dougan, S N Chatfield, M M Levine
C O Tacket
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M B Sztein
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G A Losonsky
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S S Wasserman
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J P Nataro
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R Edelman
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D Pickard
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G Dougan
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S N Chatfield
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M M Levine
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA. ctacket@umppa1.ab.umd.edu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 
  • Article
  • Info & Metrics
  • PDF
Loading

ABSTRACT

A single-dose, oral Salmonella typhi vaccine strain has been sought as a carrier or vector of cloned genes encoding protective antigens of other pathogens. Such a hybrid vaccine, administered orally, would stimulate immune responses both at the mucosal surface and in the systemic compartment and would potentially provide protection against multiple pathogens. S. typhi CVD 908 and CVD 906, which harbor deletions in aroC and aroD, were further engineered by deletion in htrA to produce strains CVD 908-htrA and CVD 906-htrA, which are unable to sustain growth and are severely impaired in their ability to survive in host tissues. These strains were fed to humans at doses of 5 x 10(7) to 5 x 10(9) CFU with buffer, and safety and immune responses were assessed. CVD 908-htrA and CVD 906-htrA were well tolerated in volunteers; mild diarrhea in 3 of 36 volunteers and mild fever in 1 volunteer were the only notable adverse responses. The vaccine strains were not detected in blood cultures and only transiently detected in stool. Serum immune responses to S. typhi lipopolysaccharide and H antigens were observed in 75 to 100% of volunteers who received 5 x 10(8) to 5 x 10(9) CFU, and cells secreting S. typhi-specific antibodies were found in all volunteers after ingestion of either strain. Sixty-three percent to 83% of volunteers developed lymphoproliferative responses to S. typhi flagellar and particulate antigens after the higher doses. These studies demonstrate the potential of CVD 908-htrA as a live vector for the delivery of heterologous genes, and a clinical trial of such a construct is planned.

PreviousNext
Back to top
Download PDF
Citation Tools
Safety of live oral Salmonella typhi vaccine strains with deletions in htrA and aroC aroD and immune response in humans.
C O Tacket, M B Sztein, G A Losonsky, S S Wasserman, J P Nataro, R Edelman, D Pickard, G Dougan, S N Chatfield, M M Levine
Infection and Immunity Feb 1997, 65 (2) 452-456; DOI:

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Infection and Immunity article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Safety of live oral Salmonella typhi vaccine strains with deletions in htrA and aroC aroD and immune response in humans.
(Your Name) has forwarded a page to you from Infection and Immunity
(Your Name) thought you would be interested in this article in Infection and Immunity.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Safety of live oral Salmonella typhi vaccine strains with deletions in htrA and aroC aroD and immune response in humans.
C O Tacket, M B Sztein, G A Losonsky, S S Wasserman, J P Nataro, R Edelman, D Pickard, G Dougan, S N Chatfield, M M Levine
Infection and Immunity Feb 1997, 65 (2) 452-456; DOI:
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About IAI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #IAIjournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0019-9567; Online ISSN: 1098-5522