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Microbial Immunity and Vaccines

Identification and Characterization of Protective T Cells in hsp65 DNA-Vaccinated and Mycobacterium tuberculosis-Infected Mice

Vania L. D. Bonato, Valeria M. F. Lima, Ricardo E. Tascon, Douglas B. Lowrie, Celio L. Silva
Vania L. D. Bonato
Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil, and
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Valeria M. F. Lima
Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil, and
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Ricardo E. Tascon
Laboratory for Mycobacterial Research, National Institute for Medical Research, London NW7 1AA, United Kingdom
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Douglas B. Lowrie
Laboratory for Mycobacterial Research, National Institute for Medical Research, London NW7 1AA, United Kingdom
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Celio L. Silva
Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil, and
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DOI: 10.1128/IAI.66.1.169-175.1998
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ABSTRACT

Immunization by intramuscular injection of plasmid DNA expressing mycobacterial 65-kDa heat shock protein (hsp65) protects mice against challenge with virulent Mycobacterium tuberculosis H37Rv. During infection or after immunization, CD4+/CD8− and CD8+/CD4− hsp65-reactive T cells increased equally in spleens. During infection, the majority of these cells were weakly CD44 positive (CD44lo) and produced interleukin 4 (IL-4) whereas after immunization the majority were highly CD44 positive (CD44hi) and produced gamma interferon (IFN-γ). In adoptive transfer of protection to naive mice, the total CD8+/CD4− cell population purified from spleens of immunized mice was more protective than that from infected mice. When the cells were separated into CD4+/CD8− and CD8+/CD4− types and then into CD44hi and CD44lo types, CD44locells were essentially unable to transfer protection, the most protective CD44hi cells were CD8+/CD4−, and those from immunized mice were much more protective than those from infected mice. Thus, whereas the CD44lo IL-4-producing phenotype prevailed during infection, protection was associated with the CD8+/CD44hiIFN-γ-producing phenotype that predominated after immunization. This conclusion was confirmed and extended by analysis of 16 hsp65-reactive T-cell clones from infected mice and 16 from immunized mice; the most protective clones, in addition, displayed antigen-specific cytotoxicity.

  • Copyright © 1998 American Society for Microbiology
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Identification and Characterization of Protective T Cells in hsp65 DNA-Vaccinated and Mycobacterium tuberculosis-Infected Mice
Vania L. D. Bonato, Valeria M. F. Lima, Ricardo E. Tascon, Douglas B. Lowrie, Celio L. Silva
Infection and Immunity Jan 1998, 66 (1) 169-175; DOI: 10.1128/IAI.66.1.169-175.1998

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Identification and Characterization of Protective T Cells in hsp65 DNA-Vaccinated and Mycobacterium tuberculosis-Infected Mice
Vania L. D. Bonato, Valeria M. F. Lima, Ricardo E. Tascon, Douglas B. Lowrie, Celio L. Silva
Infection and Immunity Jan 1998, 66 (1) 169-175; DOI: 10.1128/IAI.66.1.169-175.1998
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KEYWORDS

Bacterial Proteins
Chaperonins
Mycobacterium tuberculosis
T-Lymphocyte Subsets
tuberculosis
Vaccines, DNA

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