Article Figures & Data
Figures
- Fig. 1.
Genetic relationships between the 59 strains examined in this study. The dendrogram was generated previously by Musser et al. (8) by the average-linkage method of clustering from a matrix of coefficients of genetic distance, based on 15 metabolic enzymes. Circles indicate strains that hybridized with hmwbut not hia, squares indicate strains that hybridized withhia but not hmw, and triangles indicate strains that failed to hybridize with either hia or hmw. Blackened symbols indicate strains that hybridized with pUO38 and IS1016. The numbers and letters to the right of the symbols are strain designations. Numbers in parentheses refer to level of adherence.
- Fig. 2.
Southern blotting performed with probes forhmw sequences and hia. Chromosomal DNA was digested with BglII, separated by agarose electrophoresis, and subjected to Southern analysis. (A) Southern analysis with a probe corresponding to the promoter and 5′ coding sequence ofhmw1A. Strains by lane: 1, 12; 2, 11; 3, 3219B; 4, 3686; 5, 3242A; 6, 3219C; 7, 3655; 8, 1667; 9, 3246A; 10, 1484A; 11, 1674. Less chromosomal DNA was inadvertently loaded in lanes 7 and 9. (B) Southern analysis with a probe that represents an intragenic fragment ofhia. Strains by lane: 1, 12; 2, 11; 3, 1862; 4, 3179B; 5, 3640; 6, 3A; 7, 1860; 8, 3230B; 9, 3248A; 10, 3230A; 11, 1396B. Comparable quantities of DNA were loaded in each lane.
- Fig. 3.
Southern blotting performed with radiolabeled pUO38. Chromosomal DNA was digested with EcoRI, separated by agarose electrophoresis, and subjected to Southern analysis. Strains by lane: 1, Eagan; 2, 1862; 3, 3179B; 4, 3640; 5, 3A; 6, 1860; 7, 3230B; 8, 3248A; 9, 3230A; 10, 1396B; 11, 3232A; 12, 3639.
- Fig. 4.
Western immunoblotting performed with serum 25G against HMW1 and HMW2 or serum 36B against Hia. Whole-cell lysates were prepared, and proteins were separated on a 7.5% polyacrylamide gel. (A) Western analysis with serum 25G. Strains by lane: 1, 12; 2, 11; 3, 3219B; 4, 3686; 5, 3242A; 6, 3219C; 7, 3655; 8, 1667; 9, 3246A; 10, 1484A; 11, 1674; 12, 3894. (B) Western analysis with serum 36B. Strains by lane: 1, 12; 2, 11; 3, 11hia::kan; 4, 1862; 5, 3179B; 6, 3640; 7, 3A; 8, 1860; 9, 3230B; 10, 3248A; 11, 3230A; 12, 1396B.
