Article Figures & Data
Figures
- Fig. 1.
omp1 and pCT PCR of strains C599 and E/UW5. Primers designed for omp1 are located 108 bp upstream of the ATG initiator and ∼80 bp downstream of the TAA terminator, resulting in a PCR product of approximately 1,250 bp. Primers designed for pCT are located in ORF8, producing a PCR product of approximately 720 bp. Lanes: 1, 1-kb DNA size standards (Life Technologies, Gibco BRL); 2,omp1 PCR of C599; 3, omp1 PCR of E/UW5; 4, pCT PCR of C599; 5, pCT PCR of E/UW5; 6, negative omp1 control PCR (no template); 7, negative pCT control PCR (no template). Black-and-white digital images were captured with an Alpha Innotech Alpha-Imager 2000 and annotated by using Adobe Photoshop and Freehand V.8 for Power Macintosh.
- Fig. 2.
Southern hybridization of BamHI-digested C599 and E/UW5 total DNA probed with DIG-labeled omp1 PCR product (A) and DIG-labeled pCT (B). Lanes: 1, DIG II marker bands at (from top to bottom) 23, 9, 6, 4, 2.3, and 2 kb; 2, 1 μg of E/UW5BamHI-digested DNA; 3, 1 μg of C599BamHI-digested DNA; 4, 0.5 μg of E/UW5BamHI-digested DNA; 5, 0.5 μg of C599BamHI-digested DNA; 6, BamHI-linearized pCT (7.5 kb); 7, omp1 PCR product (1,250 bp). Southern blot X-ray images were scanned at 400 dots per inch with a UMax Powerbook scanner. The images were then combined into one photo by using Adobe Photoshop and Freehand V.8 for Power Macintosh.
- Fig. 3.
Fluorescent micrograph of chlamydial inclusion bodies stained with a fluorescein-labeled serovar E-specific anti-major outer membrane protein monoclonal antibody. McCoy cell monolayers were infected with laboratory strain E/UW5 (A) and clinical isolate C599 (B). Infected monolayers were then fixed and stained as previously described (8). Magnification, ×100.
Tables
- Table 1.
Growth rates at two independent times for clinical isolate C599 and laboratory strain E/UW5
Trial No. of IFU/100 μl E/UW5 C599 Primary passage Secondary passage Primary passage Secondary passage 1 237 861 146 25 2 218 1,757 119 1
