Investigation of the Roles of Toxin-Coregulated Pili and Mannose-Sensitive Hemagglutinin Pili in the Pathogenesis of Vibrio cholerae O139 Infection

Carol O. Tacket; Ronald K. Taylor; Genevieve Losonsky; Yu Lim; James P. Nataro; James B. Kaper; Myron M. Levine

doi:10.1128/IAI.66.2.692-695.1998

DOI: 10.1128/IAI.66.2.692-695.1998

Article Figures & Data

Tables

Table 1.

Clinical and bacteriologic responses in volunteers who received 10⁷ CFU of V. cholerae O139 strain CVD 112, KHT37 (CVD 112 ΔmshA), or KHT47 (CVD 112 ΔtcpA)

Strain	Diarrhea attack rate^a (%)	Mean diarrheal stool vol (ml)	Rate^a of vibrio excretion (%)	Geometric mean peak no. of organisms/g of stool	Rate^a of positive duodenal fluid cultures (%)	Geometric mean no. of organisms recovered from duodenal fluid
CVD 112	3/10 (30)	693	8/10 (80)	1.4 × 10⁵	3/8 (38)	1.6 × 10³
KHT 37 (CVD 112 ΔmshA)	5/10 (50)	529	8/10 (80)	1.4 × 10⁵	2/9 (22)	3.6 × 10³
KHT47 (CVD 112 ΔtcpA)	0/9 (0)^b	0	1/9 (11)^c	3 × 10²	0/7 (0)^d	0

↵a Number with result/number tested.
↵b P = 0.12 and 0.02 (Fisher’s exact tests) versus rates in CVD 112 and CVD 112 ΔmshArecipients, respectively.
↵c P < 0.01 (Fisher’s exact test) versus rates in CVD 112 and CVD 112 ΔmshA recipients.
↵d P is not significant (Fisher’s exact tests).

Table 2.

Immune responses in volunteers who received 10⁷ CFU of V. cholerae O139 strain CVD 112, KHT37 (CVD 112 ΔmshA), or KHT47 (CVD 112 ΔtcpA)

Strain	Vibriocidal^a antibody seroconversion rate^b (%)	Geometric mean peak reciprocal vibriocidal antibody titer	IgG anti-cholera toxin antibody seroconver- sion rate^b(%)	Geometric mean change in optical density for IgG anticholera toxin antibody	IgG anti-TCP antibody seroconversion rate^b (%)
CVD 112	10/10 (100)	121	10/10 (100)	0.78	2/10 (20)
KHT37 (CVD 112 ΔmshA)	8/10 (80)	106	8/10 (80)	0.63	1/10 (10)
KHT47 (CVD 112 ΔtcpA)	1/9 (11)^c	29^d	0/9 (0)^e	0.04	0/9 (0)

↵a Vibriocidal responses were measured against V. cholerae O139 strain 2L, an encapsulated mutant of strain AI1837, the parent strain of CVD 112.
↵b Number with result/number tested.
↵c P < 0.01 (Fisher’s exact tests) versus rate in recipients of either CVD 112 or KHT37 (CVD 112 ΔmshA).
↵d P < 0.01 (Student’s t test) versus titer in recipients of either CVD 112 or KHT37 (CVD 112 ΔmshA).
↵e P < 0.001 (Fisher’s exact tests) versus rate in recipients of either CVD 112 or KHT37 (CVD 112 ΔmshA).