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Bacterial Infections

Role of Streptococcal Pyrogenic Exotoxin B in the Mouse Model of Group A Streptococcal Infection

Chih-Feng Kuo, Jiunn-Jong Wu, Kuei-Yuan Lin, Pei-Jane Tsai, Shiour-Ching Lee, Ying-Tai Jin, Huan-Yao Lei, Yee-Shin Lin
Chih-Feng Kuo
Departments of Microbiology and Immunology,
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Jiunn-Jong Wu
Medical Technology, and
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Kuei-Yuan Lin
Departments of Microbiology and Immunology,
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Pei-Jane Tsai
Departments of Microbiology and Immunology,
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Shiour-Ching Lee
Departments of Microbiology and Immunology,
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Ying-Tai Jin
Pathology, National Cheng Kung University Medical College, Tainan, Taiwan, Republic of China
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Huan-Yao Lei
Departments of Microbiology and Immunology,
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Yee-Shin Lin
Departments of Microbiology and Immunology,
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DOI: 10.1128/IAI.66.8.3931-3935.1998
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ABSTRACT

Streptococcal pyrogenic exotoxin B (SPE B) is a cysteine protease produced by Streptococcus pyogenes. In this study, the differences in virulence between protease-positive clinical isolates and their protease-negative mutants were examined in a mouse model. Isogenic protease-negative mutants were constructed by homologous recombination, using integrational plasmids to disrupt thespeB gene. These mutants caused less mortality and tissue damage than protease-positive strains when inoculated into BALB/c mice via air pouch, suggesting that SPE B cysteine protease plays an important role in the pathogenesis of S. pyogenesinfection. Reconstitution of SPE B in the air pouches increased the mortality of mice receiving the speB mutant strain. Infiltrated cell numbers in the exudates from the air pouches of mice infected with SPE B-producing S. pyogenes were higher than those from mice infected with protease-negative mutants at 12 h. However, despite pretreatment with vinblastine to deplete neutrophils, injection of protease-positive bacteria still resulted in severe tissue injury, indicating that neutrophil infiltration may not be the major factor involved in SPE B-enhanced tissue damage. The role of SPE B was further confirmed by demonstrating that SPE B immunization of mice conferred protection from challenge with a lethal dose of protease-positive bacteria.

  • Copyright © 1998 American Society for Microbiology
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Role of Streptococcal Pyrogenic Exotoxin B in the Mouse Model of Group A Streptococcal Infection
Chih-Feng Kuo, Jiunn-Jong Wu, Kuei-Yuan Lin, Pei-Jane Tsai, Shiour-Ching Lee, Ying-Tai Jin, Huan-Yao Lei, Yee-Shin Lin
Infection and Immunity Aug 1998, 66 (8) 3931-3935; DOI: 10.1128/IAI.66.8.3931-3935.1998

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Role of Streptococcal Pyrogenic Exotoxin B in the Mouse Model of Group A Streptococcal Infection
Chih-Feng Kuo, Jiunn-Jong Wu, Kuei-Yuan Lin, Pei-Jane Tsai, Shiour-Ching Lee, Ying-Tai Jin, Huan-Yao Lei, Yee-Shin Lin
Infection and Immunity Aug 1998, 66 (8) 3931-3935; DOI: 10.1128/IAI.66.8.3931-3935.1998
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KEYWORDS

Bacterial Proteins
Cysteine Endopeptidases
Exotoxins
membrane proteins
Streptococcal Infections
Streptococcus pyogenes

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