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Bacterial Infections

Role of Streptococcal Pyrogenic Exotoxin B in the Mouse Model of Group A Streptococcal Infection

Chih-Feng Kuo, Jiunn-Jong Wu, Kuei-Yuan Lin, Pei-Jane Tsai, Shiour-Ching Lee, Ying-Tai Jin, Huan-Yao Lei, Yee-Shin Lin
Chih-Feng Kuo
Departments of Microbiology and Immunology,
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Jiunn-Jong Wu
Medical Technology, and
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Kuei-Yuan Lin
Departments of Microbiology and Immunology,
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Pei-Jane Tsai
Departments of Microbiology and Immunology,
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Shiour-Ching Lee
Departments of Microbiology and Immunology,
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Ying-Tai Jin
Pathology, National Cheng Kung University Medical College, Tainan, Taiwan, Republic of China
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Huan-Yao Lei
Departments of Microbiology and Immunology,
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Yee-Shin Lin
Departments of Microbiology and Immunology,
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DOI: 10.1128/IAI.66.8.3931-3935.1998
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    Fig. 1.

    Survival of S. pyogenes-infected mice after inoculation in air pouches with 4 × 109 CFU of wild-type A-20 (n = 21) or its speB mutant, SW 507 (n = 23) (A), or wild-type NZ131 (n = 12) or its speB mutant, SW510 (n = 15) (B).

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    Fig. 2.

    Skin tissue sections from mice 48 h after inoculation in air pouches with 109 CFU of S. pyogenes A-20 (A), SW507 (B), NZ131 (C), SW510 (D), A-20 in mice pretreated with vinblastine (E), and SW507 in mice pretreated with vinblastine (F). Magnification, ×24 (A, B, E, and F) and ×60 (C and D). The epidermis, subcutaneous fat, and muscle fibers were severely damaged or destroyed in skin tissues infected by wild-type strains with or without vinblastine pretreatment but not in those infected by thespeB mutants.

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    Fig. 3.

    Numbers of cells collected from the exudates of the air pouches of mice injected with 109 CFU of A-20 (n = 18), NZ131 (n = 7), and theirspeB mutants, SW507 (n = 18) and SW510 (n = 7), 12 h postinjection. ∗,P < 0.05; ∗∗, P < 0.01 (by Student’s t test).

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    Fig. 4.

    Protection against skin infection by protease-positiveS. pyogenes by immunization with SPE B. Mice were immunized with purified SPE B, with nonimmunized mice as controls, before A-20 challenge. Only those that showed titers of anti-SPE B 100-fold or more higher than those of the nonimmunized group were used for experiments.n = 5 mice/group.

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  • Table 1.

    Mortality in SW507-infected mice with or without SPE B in the air pouch

    Expt no.InoculationMortality (no. dead/total)
    Day 2Day 4Day 6
    1aSW507 + saline0/120/120/12
    SW507 + SPE B7/1814/1814/18
    2bSW507 + saline0/60/60/6
    SW507 + SPE B4/109/1010/10
    SW507 + SPE B (heat inactivated)0/80/80/8
    • ↵a BALB/c mice were inoculated in the air pouches with 4 × 108 CFU of A-20 speBmutant SW507, and 150 μl of purified SPE B (1 mg/ml) or an equivalent volume of saline was also inoculated into the air pouches, at both 0 and 3 h.

    • ↵b In one group, SPE B was heat inactivated at 60°C for 20 to 30 min before inoculation into the air pouches.

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Role of Streptococcal Pyrogenic Exotoxin B in the Mouse Model of Group A Streptococcal Infection
Chih-Feng Kuo, Jiunn-Jong Wu, Kuei-Yuan Lin, Pei-Jane Tsai, Shiour-Ching Lee, Ying-Tai Jin, Huan-Yao Lei, Yee-Shin Lin
Infection and Immunity Aug 1998, 66 (8) 3931-3935; DOI: 10.1128/IAI.66.8.3931-3935.1998

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Role of Streptococcal Pyrogenic Exotoxin B in the Mouse Model of Group A Streptococcal Infection
Chih-Feng Kuo, Jiunn-Jong Wu, Kuei-Yuan Lin, Pei-Jane Tsai, Shiour-Ching Lee, Ying-Tai Jin, Huan-Yao Lei, Yee-Shin Lin
Infection and Immunity Aug 1998, 66 (8) 3931-3935; DOI: 10.1128/IAI.66.8.3931-3935.1998
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KEYWORDS

Bacterial Proteins
Cysteine Endopeptidases
Exotoxins
membrane proteins
Streptococcal Infections
Streptococcus pyogenes

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