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MOLECULAR AND CELLULAR PATHOGENESIS

The Link between Phylogeny and Virulence inEscherichia coli Extraintestinal Infection

Bertrand Picard, José Sevali Garcia, Stéphanie Gouriou, Patrick Duriez, Naïma Brahimi, Edouard Bingen, Jacques Elion, Erick Denamur
Bertrand Picard
Laboratoire de Microbiologie, Hôpital Morvan, Brest,
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José Sevali Garcia
Laboratoire de Microbiologie, Hôpital Cochin, Assistance Publique—Hôpitaux de Paris,
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Stéphanie Gouriou
Laboratoire de Microbiologie, Hôpital Morvan, Brest,
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Patrick Duriez
INSERM U 458 and
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Naïma Brahimi
Laboratoire de Recherche en Bactériologie Pédiatrique (JE 2255), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris, France
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Edouard Bingen
Laboratoire de Recherche en Bactériologie Pédiatrique (JE 2255), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris, France
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Jacques Elion
INSERM U 458 and
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Erick Denamur
INSERM U 458 and
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DOI: 10.1128/IAI.67.2.546-553.1999
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    Fig. 1.

    Factorial analysis of correspondence of the 72 strains of the ECOR collection (+) based on the rrn RFLP data (9). The 82 E. coli clinical isolates belonging to groups 1 (▴), 2 (■), 3 (▾), 4 (▵), 5 (□), and 6 (▿), considered supplementary observations, are projected in the F1/F2 plane. This plane, obtained by computation, is defined by the two principal axes of the analysis; the first axis, F1, explains most of the variance, and the second axis, F2 (orthogonal to F1), explains most of the variance which is not explained by F1. Phylogenetic groups A, B1, B2, and D (17) of E. coli are indicated. For clarity, when several strains are projected on the same point, only one is represented.

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    Fig. 2.

    Graphical representation of the results of the FAC carried out with the whole data from the 82 E. coli clinical strains. (A) Projections of the variables on the F1/F2 plane (defined as in Fig. 1): hly, detection of the hly operon; aer, detection of the aer operon; sfa, detection of thesfa/foc operon; ibe, detection of the ibe10 gene; pap, detection of the pap operon, afa, detection of theafa operon; K1, detection of the K1 antigen; b1, carboxylesterase B type B1; b2, carboxylesterase B type B2; B2, phylogenetic group B2; B1, phylogenetic group B1; A, phylogenetic group A; D, phylogenetic group D; pat, pathologic origin; co, commensal origin; 0/1, low level of lethality to mice; 2/8, medium level of lethality to mice; 9/10, high level of lethality to mice. The + and − signs after the variables indicate the presence or absence of the variable, respectively. (B) Projections of the 82 E. coli strains on the F1/F3 plane. The third axis F3, orthogonal to the F1/F2 plane, explains the largest part of the variance which is not explained by the F1/F2 plane. The strains (▴) are numbered as in Table 1, and for clarity, when several strains are projected on the same point, only one is represented.

Tables

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  • Table 1.

    Characteristics of the 82 E. coliclinical strainsa

    StrainOriginCarboxylesterase B ( MF)Expression of:GroupDetection ofPhylogenetic group
    HemolysinMRHAK1 antigensfa/focoperonpap operonafaoperonhly operonaeroperonibe10 geneLethality in mice
    1Feces70−01−−−−−−−0B1
    2Feces70−01−−−−−−−0B1
    3Feces70−01−−−−−−−0B1
    4Feces70−01−−−−−−−0B1
    5Feces70−01−−−−−+−0B1
    6Feces70−01+−−−−−−0A
    7Feces70−01−−−−−−−0B1
    8Feces70−01−−−−−−−0A
    9Feces70−01−−−−−−−0B1
    10Feces70−01−−−−−−−0A
    11Feces70−01−−−−−+−0A
    12Feces70−01−−−−−−−0B1
    13Feces70−01−−−−−−−0A
    14Feces70−01−−−−−+−0A
    15Feces70−01−−−−−−−0A
    16Blood72−02−−−−−−−0A
    17Urine72−02−−−−−−−0A
    18Urine70−02−−+−+−−9B1
    19Urine70−02−−−−−−−0A
    20Miscellaneous68−02−−−−−−−0A
    21Urine72−02−−−−−−−0B1
    22Urine70−02−−−−−−−0A
    23Urine70−02−−−−−−−0A
    24Urine70−02−−−−−−−0B1
    25Urine70−02+−−−−−−0A
    26Urine70−02−−−−−−−0B1
    27Urine70−02−−−−−−−0B1
    28Urine70−02−−−−−−−0A
    29Urine70−02−−−−−−−0A
    30Urine70−02−−−−−−−0B1
    31Urine70−02−−−−−−−0B1
    32Urine70−02−−−−−−−0B1
    33Urine68−02−−−−−−−0B1
    34Blood73−43−−−−−−−0A
    35Blood72−23−−++−−−10+D
    36Miscellaneous70−33−−+−−+−10+B1
    37Miscellaneous70−23−−−+−+−0A
    38Miscellaneous68−23−−+−−+−10+D
    39Urine72−23+−−−−+−10+B1
    40Urine70−33−−−+−+−0A
    41Urine72−33−−+−−−−0B1
    42Urine70−33−−+−−−−5B1
    43Urine70−43−−+−−+−0B1
    44Urine70−23−−−−−−−7A
    45Urine70−33−−−−−−−0B1
    46Urine62−04+−+−−+−9B2
    47Urine62−04−−−−−−−0B2
    48Blood60−04−−−−−−−0B2
    49Urine57−04−−−−−−+10+B2
    50Urine57−04−−−−−+−0B2
    51Urine57−04++−−−−−10+B2
    52Urine57−04++−−−−−10+B2
    53Urine57−04+−−−−++0B2
    54Urine57−04−−−−+−−0B2
    55Urine57−04−−+−−−+10B2
    56Urine57−35+−+−−+−9B2
    57Urine57−15−+−−+−−9+B2
    58Urine63−25+−−−−−−9+B2
    59Urine62−25+−+−−+−10+B2
    60Blood62−25+−+−−+−1B2
    61Miscellaneous62−25+−+−−+−9B2
    62Urine57−35−++−−−−10+B2
    63Urine57−25−++−−−−10+B2
    64Urine57−15−++−−−+10+B2
    65Miscellaneous62−25+−+−−−−10+B2
    66Urine60−35+−+−−+−9+B2
    67Urine57−35−−+−−+−0B2
    68Urine57−35−++−−+−3B2
    69Miscellaneous62−25−−+−−+−10+B2
    70Urine57−35−++−−−+10+B2
    71Urine62+36−++−++−10+B2
    72Urine57+26−−−−+−−10+B2
    73Blood57+26−++−+−−10+B2
    74Urine57+36+++−+−−10+B2
    75Miscellaneous57+36−++−+−−10+B2
    76Urine57+26−++−+−−3B2
    77Urine60+26−++−+−−10+B2
    78Urine57+46−−−−++−5B2
    79Urine57+36−−+−++−10+B2
    80Urine57+36−−+−++−10+B2
    81Urine57+46−−+−++−9+B2
    82Urine57+36−++−+−−10+B2
    • ↵a Strains originating from feces are commensal (group 1), while the remainder were isolated from patients with pathological conditions (groups 2 to 6). Strains exhibiting a B1-type carboxylesterase B (MF ≈ 68 to 72) correspond to groups 1 to 3, while strains exhibiting a B2-type carboxylesterase B (MF ≈ 57 to 63) correspond to groups 4 to 6. MHRA was quantified from 0 to 4 according to the intensity of the reaction. The six groups (see Materials and Methods) are listed in order. The lethality in mice is indicated as the number of mice killed by the bacterial strain (0 to 10). A plus sign indicates that the bacterial strain killed at least six mice in less than 18 h. Phylogenetic groups A, B1, B2, and D are indicated as in reference 17.

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The Link between Phylogeny and Virulence inEscherichia coli Extraintestinal Infection
Bertrand Picard, José Sevali Garcia, Stéphanie Gouriou, Patrick Duriez, Naïma Brahimi, Edouard Bingen, Jacques Elion, Erick Denamur
Infection and Immunity Feb 1999, 67 (2) 546-553; DOI: 10.1128/IAI.67.2.546-553.1999

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The Link between Phylogeny and Virulence inEscherichia coli Extraintestinal Infection
Bertrand Picard, José Sevali Garcia, Stéphanie Gouriou, Patrick Duriez, Naïma Brahimi, Edouard Bingen, Jacques Elion, Erick Denamur
Infection and Immunity Feb 1999, 67 (2) 546-553; DOI: 10.1128/IAI.67.2.546-553.1999
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KEYWORDS

Escherichia coli
Escherichia coli Infections

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