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MOLECULAR AND CELLULAR PATHOGENESIS

Neisseria gonorrhoeae Mutants Altered in Toxicity to Human Fallopian Tubes and Molecular Characterization of the Genetic Locus Involved

Cindy Grove Arvidson, Risa Kirkpatrick, Manon T. Witkamp, Jason A. Larson, Christel A. Schipper, Lillian S. Waldbeser, Peadar O’Gaora, Morris Cooper, Magdalene So
Cindy Grove Arvidson
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, and
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Risa Kirkpatrick
Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 62794
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Manon T. Witkamp
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, and
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Jason A. Larson
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, and
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Christel A. Schipper
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, and
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Lillian S. Waldbeser
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, and
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Peadar O’Gaora
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, and
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Morris Cooper
Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 62794
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Magdalene So
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, and
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DOI: 10.1128/IAI.67.2.643-652.1999
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ABSTRACT

In an effort to identify potential cytotoxins expressed byNeisseria gonorrhoeae, we have identified a locus that, when mutated in the gonococcus, results in a significant increase in toxicity of the strain to human fallopian tube organ cultures (HFTOC). This locus, gly1, contains two open reading frames (ORFs) which are likely cotranscribed. ORF1 encodes a polypeptide of 17.8 kDa with a signal sequence that is recognized and processed inEscherichia coli and N. gonorrhoeae. The 15.6-kDa processed polypeptide has been observed in membrane fractions and filtered spent media from cultures of E. coli expressing gly1 and in outer membrane preparations of wild-type N. gonorrhoeae. The gly1 locus is not essential for bacterial survival, and it does not play a detectable role in epithelial cell adhesion, invasion, or intracellular survival. However, agly1 null mutant causes much more damage to fallopian tube tissues than its isogenic wild-type parent. A strain complemented intrans for the gly1 mutation showed a level of toxicity to HFTOC similar to the level elicited by the wild-type parent. Taken together, these results indicate an involvement of the gly1 locus in the toxicity of N. gonorrhoeae to human fallopian tubes.

  • Copyright © 1999 American Society for Microbiology
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Neisseria gonorrhoeae Mutants Altered in Toxicity to Human Fallopian Tubes and Molecular Characterization of the Genetic Locus Involved
Cindy Grove Arvidson, Risa Kirkpatrick, Manon T. Witkamp, Jason A. Larson, Christel A. Schipper, Lillian S. Waldbeser, Peadar O’Gaora, Morris Cooper, Magdalene So
Infection and Immunity Feb 1999, 67 (2) 643-652; DOI: 10.1128/IAI.67.2.643-652.1999

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Neisseria gonorrhoeae Mutants Altered in Toxicity to Human Fallopian Tubes and Molecular Characterization of the Genetic Locus Involved
Cindy Grove Arvidson, Risa Kirkpatrick, Manon T. Witkamp, Jason A. Larson, Christel A. Schipper, Lillian S. Waldbeser, Peadar O’Gaora, Morris Cooper, Magdalene So
Infection and Immunity Feb 1999, 67 (2) 643-652; DOI: 10.1128/IAI.67.2.643-652.1999
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KEYWORDS

Bacterial Proteins
bacterial toxins
cytotoxins
Fallopian Tubes
Neisseria gonorrhoeae

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