Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Infection and Immunity
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
MOLECULAR AND CELLULAR PATHOGENESIS

Neisseria gonorrhoeae Coordinately Uses Pili and Opa To Activate HEC-1-B Cell Microvilli, Which Causes Engulfment of the Gonococci

J. McLeod Griffiss, Claudia J. Lammel, Jun Wang, Nusi P. Dekker, G. F. Brooks
J. McLeod Griffiss
Centre for Immunochemistry and
Department of Laboratory Medicine, University of California, San Francisco, California 94143
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Claudia J. Lammel
Department of Laboratory Medicine, University of California, San Francisco, California 94143
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun Wang
Centre for Immunochemistry and
Department of Laboratory Medicine, University of California, San Francisco, California 94143
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nusi P. Dekker
Department of Laboratory Medicine, University of California, San Francisco, California 94143
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G. F. Brooks
Department of Laboratory Medicine, University of California, San Francisco, California 94143
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/IAI.67.7.3469-3480.1999
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

ABSTRACT

This study was undertaken to examine concomitant roles of pili and colony opacity-associated proteins (Opa) in promoting Neisseria gonorrhoeae adherence to and invasion of human endometrial HEC-1-B cells. Adherence of N. gonorrhoeae to cultured HEC-1-B cells was saturable, even though organisms adhered to <50% of the cells. During 4 to 6 h of incubation, adherent mono- and diplococci formed microcolonies on the surfaces of the cells. Microvilli of the HEC-1-B cells adhered by their distal ends to individual cocci within the microcolonies. When the microcolonies grew from isogenic pilus-negative (P−) Opa−, P− Opa+, or P+ Opa−gonococci, microvilli did not elongate, and the colonies were not engulfed. In contrast, the microvilli markedly elongated during exposure to P+ Opa+ gonococci. The microvilli adhered to the organisms along their full lengths and appeared to actively participate in the engulfment of the microcolonies. Internalized microcolonies, with P+ Opa+gonococci, contained dividing cocci and appeared to be surrounded by cell membrane but were not clearly within vacuoles. In contrast, degenerate individual organisms were within vacuoles. Low doses of chloramphenicol, which inhibits protein synthesis by both prokaryotes and eukaryotes, prevented the microvillar response to and internalization of the P+ Opa+ gonococci; higher doses caused internalization without microvillus activation. Cycloheximide and anisomycin, which inhibit only eukaryotic protein synthesis, caused dose-dependent enhancement of uptake. Cytochalasins reduced engulfment; colchicine had no effect. These results show that gonococci must express both pili and Opa to be engulfed efficiently by HEC-1-B cells.

Pili and colony opacity-associated proteins (Opa) are surface-exposed structures of Neisseria gonorrhoeae. Pili can extend several micrometers from the gonococcal cell surface, while Opa proteins are integral parts of the outer membrane. Pili and Opa have been studied individually and extensively for their relative roles in gonococcal adherence to and invasion of host epithelial cells.

Pili occur in small-colony variants of gonococci that can be selectively subcultured with a dissecting microscope (30, 32, 33, 68). Pili appear to mediate interactions between gonococci and host epithelial cells (42, 43, 53, 54, 63, 73). Pili are inhibitory to phagocytosis of gonococci by polymorphonuclear cells (18, 22, 48), but their effect on internalization by epithelial cells is unclear. Pili are composed of PilE, the major pilus subunit; PilC, an adherence-associated protein; and possibly other components (1, 54, 55). Gonococci undergo pilus phase variation, converting from pilus positive (P+) to P− and from P− to P+ in vitro and quite probably in vivo as well (67, 68). Transcellular passage through HEC-1-B cells in culture yields pilus phase variation from P+ to P− (26). In vitro, pili undergo rapid antigenic variation; such antigenic variation undoubtedly occurs in vivo also.

Opa proteins are heat-modifiable outer membrane proteins found in gonococcal colony color variants (64, 65). Opa proteins routinely are found in gonococci in opaque colonies and sometimes are found in gonococci in transparent colonies; however, Opa−gonococci form only transparent colonies, and these are generally similar to Opa+ transparent colonies when viewed under a dissecting microscope (17). Opa proteins confer upon the gonococci variation in surface appearance and size (17). It has been estimated that each strain of gonococcus has 9 to 12opa genes (4, 14). Each Opa has hypervariable 1 (HV1) and HV2 regions (4, 14); the genetic codes for the HV1 and HV2 regions may appear more than once in a strain of gonococcus and in diverse strains as well (4, 8, 14). Shifts in reading the repetitive sequences within each opa locus result in frequent changes (≥1 in 103) in the expression of Opa proteins (40, 44, 61). Opa proteins have an important role in gonococcal adherence to host cells. Adherence of P−Opa+ gonococci to epithelial cells in vitro involves heparan sulfate (11). Opa proteins confer on gonococci cell tropism, with some Opa proteins promoting adherence to polymorphonuclear cells and others promoting adherence to specific lines of epithelial or other cells in vitro (7, 12, 13, 34, 36). Opa proteins differentially recognize members of the carcinoembryonic antigen family (70), which may result in the tissue tropism (7, 12, 13).

Invasion requires the active participation of both the gonococcus and the host cell (10, 52, 58). During invasion, epithelial cell microvilli surround the bacteria as they are drawn into the eukaryotic cell (52, 58). Endocytosis is stopped by drugs that block actin filament rearrangement within the host cell (3, 10, 58). The gonococcal factors that signal the host cell to engulf them have not been defined completely but probably include Opa proteins (39, 54, 59, 74).

There is considerable indirect evidence that pili and Opa are concomitantly important in the infectious process. Gonococci have to be piliated to be infectious for human fallopian tube tissues in organ culture and for infection of male human volunteers (32, 33, 42, 69, 73). Piliated gonococci probably are present in vivo (28) and are present in primary clinical cultures from infected patients (30, 35, 60, 68). One to four Opa proteins are present in primary clinical isolates from the male urethra and rectum and from the cervix (27), whereas Opa−isolates either are not seen (27) or are uncommon (21, 29). Human male volunteers inoculated intraurethrally with Opa− gonococci almost always shed Opa+gonococci on subsequent cultures (31, 56, 66). Both pili and Opa have been implicated in the signaling of epithelial cells to engulf gonococci (23, 25, 39, 54, 59, 74).

This study was undertaken to examine concomitant roles of pili and Opa in promoting adherence to and invasion of HEC-1-B cells. The data indicate that gonococci use pili and Opa coordinately in their interactions with the HEC-1-B cells.

MATERIALS AND METHODS

Gonococcal strains.Three sets of N. gonorrhoeaestrains were used in this study. The organisms were subcultured onto GC Agar Base supplemented with IsoVitaleX (BBL, Cockeysville, Md.) (GC agar). A dissecting microscope and standard criteria were used to select piliated (P+) and nonpiliated (P−) colony types with Opa proteins (Opa+) and without Opa proteins (Opa−).

The P− and P+ variants of strain MS11mk (4, 66) were kindly supplied by Herman Schneider (Walter Reed Army Institute of Research, Washington, D.C.). The nonrevertant P− MS11mk mutant lacked a complete pilE site and could not rearrange pilSloci; it was derived by exhaustive selection from the wild-type strain. A second, heavily piliated variant of MS11mk was also selected; it was designated P+. These MS11mkvariants had transparent colony phenotypes and were Opa−(66); opaque colony variants that were Opa+ were selected from each of the P− and P+ variants.

Schneider et al. (56, 57) observed that the variant of MS11mk that expresses C lipooligosaccharides is virulent for the male urethra. This variant was obtained from Herman Schneider, and variants of it with piliated and Opa+ phenotypes were selected, as described above.

Opa variants of strains F62-SF and FA1090 have been extensively described (5, 16). Janne Cannon (University of North Carolina, Chapel Hill) kindly provided the FA1090 Opa variants. Nonpiliated variants of FA1090 Opa− and piliated and nonpiliated variants of FA1090 OpaE were selected (17). Piliated and nonpiliated variants of F62-SF OpaA and Opa−also were selected.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting.Pilus and Opa expression was monitored by solubilizing in sodium dodecyl sulfate whole-cell lysates of the gonococci used in each adherence assay and electrophoresing them through a 12.5% acrylamide separating gel (37). The proteins then were transferred to nitrocellulose and incubated with antibodies specific for pilin and Opa proteins (9, 38).

The Opa content was determined by comparing lysates prepared at 37 and 100°C and looking for the presence of a heat-modifiable protein. Transblots of FA1090 lysates were incubated with monoclonal antibodies that bind each of its Opa proteins (2) (kindly provided as hybridoma supernatants by Janne Cannon). Opa proteins of the other gonococcal strains were detected in immunoblots with use of monoclonal antibody B33, which reacts with all Opa proteins (38).

Rabbit antipilin antibody against the CNBr fragment 2 of strain R10 pili kindly was provided by Gary Schoolnik (Stanford University, Palo Alto, Calif.). This antibody was incubated with transblots of gonococcal lysates to confirm whether or not the organisms made pili.

Adherence assays.HEC-1-B cells, a human endometrial adenocarcinoma cell line (ATCC HTB 113; American Type Culture Collection, Rockville, Md.), were maintained in Eagle minimal essential medium (MEM) containing Earle’s salts, nonessential amino acids, sodium pyruvate, and 10% fetal calf serum (FCS). To use them in adherence assays, the HEC-1-B cells were trypsinized from the surface of a tissue culture flask and suspended in tissue culture medium at 2 × 105 to 3 × 105 cells/ml, and 1 ml was plated onto each round Thermanox coverslip (Electron Microscopy Sciences, Fort Washington, Pa.) in 24-well tissue culture trays (Falcon; Becton Dickinson Labware, Lincoln Park, N.J.). The cells were incubated for 20 to 24 h at 37°C in 5% CO2. Gonococcal inocula were suspended in MEM containing 0.05% HEPES buffer at pH 7.4.

For studies in which light microscopy was used to assess adherence and microcolony formation, P+ Opa+MS11mkC and P+ FA1090 OpaE gonococci were inoculated onto HEC-1-B cells that had been plated, as described above, onto tissue culture chamber slides (four-well, Lab-Tek; Miles Scientific, Naperville, Ill.) at inocula of 1 × 104to 2 × 104 to 2 × 107 CFU/ml. The inocula were removed at selected times of incubation, and the slides were rinsed with phosphate-buffered saline (PBS) to remove nonadherant bacteria and then Gram stained.

For studies in which electron microscopy (EM) was used to assess adherence, gonococci were inoculated onto the coverslips at approximately 2 × 107 CFU/ml. The inocula were removed after 30 min or 1 h of incubation, and the coverslips were rinsed to remove nonadherent bacteria. The incubation was continued after the addition of fresh medium. The coverslips were rinsed again at selected times and fixed at 4°C in 2.5% glutaraldehyde in 0.1 M cacodylate buffer of pH 7.38 for 4 h. The coverslips were held in the cacodylate buffer until they were prepared for scanning EM (SEM).

When culture was used to assess adherence, 2 × 105HEC-1-B cells were plated directly into each well of the 24-well tissue culture trays. The gonococcal strains were used at inocula of 1 × 108 to 2 × 108 CFU/ml. The HEC-1-B cells were incubated with the bacteria for 2 to 3 h and then rinsed four times with PBS to remove nonadherant bacteria. The HEC-1-B cells were dislodged from the wells with trypsin and lysed in 1% saponin with vortexing. The lysates were mixed by vortex action and then diluted in PBS. Aliquots of appropriate dilutions were plated onto GC agar, and the colonies were counted after overnight incubation. The assays were done in duplicate or triplicate.

SEM.For SEM, the coverslips were prepared as previously described (16). The cells were postfixed in 2% (vol/vol) OsO4 in 0.1 M cacodylate buffer and placed sequentially in 1% (vol/vol) aqueous tannic acid and 2% (wt/vol) uranyl acetate, with rinse steps between each solution. The cells were dehydrated by placing them in a series of graded ethanol solutions (50 to 100%). The coverslips were rinsed in hexamethyldisilazane (Electron Microscopy Sciences), mounted on SEM stubs, and sputter coated with 25 nm of platinum. A scanning electron microscope (DS130; International Scientific Instruments, Milpitas, Calif.) was used to examine the specimens.

Invasion assays.Assays for invasion differed from assays for adherence in that the HEC-1-B cells were incubated with gonococcal variants for 10 h before being processed for transmission EM (TEM) and for 6 h before being processed for culture. The coverslips were rinsed and fixed in glutaraldehyde-cacodylate prior to preparation for TEM. When culture was used to assess invasion, gentamicin (250 μg/ml), which kills extracellular but not intracellular gonococci, was added to the medium after 4 h of incubation, and the incubation was continued for an additional 2 h, after which the HEC-1-B cells were processed and quantitative cultures of intracellular organisms were done, as for the adherence assays (see above).

TEM.For TEM, the cell layers on the coverslips were fixed in glutaraldehyde and OsO4 as described for SEM and then transferred to cacodylate buffer in glass microbeakers. The cell layers were dehydrated in a graded acetone series and infiltrated with resin (Araldite/Medcast; Ted Pella, Redding, Calif.). After 3 h of infiltration in whole resin, the coverslips containing the cell layers were placed on a glass surface, and several rectangles were cut out of the centers of the coverslips. The rectangles were placed cell side up in flat embedding molds with fresh resin and polymerized overnight at 60°C. Cross sections of the cells were obtained by sectioning through the embedded coverslips. Sections were placed on bare 150-mesh copper grids, stained with uranyl acetate and lead citrate, and viewed with an electron microscope (100CX; JEOL USA, Medford, Mass.).

Inhibitors.Chloramphenicol, cycloheximide, and anisomycin were used to inhibit protein synthesis, cytochalasin B and D were used to block actin polymerization, and colchicine was used to block tubulin polymerization. All except chloramphenicol, which was obtained from the hospital pharmacy, were from Sigma (St. Louis, Mo.).

Inhibitors were dissolved in either dimethyl sulfoxide or MEM (chloramphenicol, cycloheximide, and anisomycin) as stock solutions, aliquoted, and stored at −20°C. Prior to use reactants were diluted in MEM supplemented with 10% (vol/vol) FCS and added to the cultured HEC-1-B cells. The highest concentration of dimethyl sulfoxide that resulted from the dilutions was prepared without inhibitor in MEM with FCS and included as a control. The highest concentration of each reactant used was incubated with the organisms for the same period in a well without HEC-1-B cells to find out whether it affected bacterial survival or growth.

Antagonist assay.HEC-1-B cells on coverslips were incubated at 37°C for 30 min in chloramphenicol at concentrations of 0 to 50 μg/ml. The spent medium was aspirated, and the cells were inoculated with gonococci diluted in medium containing chloramphenicol and incubated for an additional hour. After the nonadherant gonococci had been removed, fresh medium with chloramphenicol was added. Coverslips were removed at specified times and fixed for EM.

Culture was used to assess the inhibitory effects of cycloheximide, cytochalasins, and colchicine; 100 ml of the dilutions of each inhibitor was added to wells that contained HEC-1-B cells so as to achieve a range of concentrations in final volumes of 1 ml. The cells were incubated with the reactants for 1 h, after which the bacteria were added and the incubation was continued for 3 to 4 h more. The trays then were processed as for the invasion assays (see above).

RESULTS

Gonococcal adherence to HEC-1-B cells is selective and saturable.Adherence of P+ Opa+N. gonorrhoeae to HEC-1-B cells was saturable. Approximately 104 gonococci, as represented by CFU, were adherent to 2 × 105 to 3 × 105 HEC-1-B cells 4 h after inoculation, even when inocula of 106 or 107 were used (Table 1). As seen by light microscopy of Gram-stained tissue cultures, gonococci adhered to <50% of the individual HEC-1-B cells, even when inocula of >106 were used (Fig. 1A). Uninfected HEC-1-B cells were not visually different from infected ones.

View this table:
  • View inline
  • View popup
Table 1.

Adherence to and invasion of HEC-1-B cells by P+ Opa+ variants of strains MS11mkC and FA1090 OpaE after 4 h of incubation

Fig. 1.
  • Open in new tab
  • Download powerpoint
Fig. 1.

Gonococci form microcolonies on or in HEC-1-B cells. Representative micrographs of Gram-stained cell monolayers after 30 min (A), 60 min (B), 90 min (C), 2 h (D), and 4 h (E) of incubation are shown. Fewer than half of the cells appear to be colonized by gonococci. The gonococcal strain is FA1090 OpaE. Representative colonies are indicated by arrowheads; other colonies are also visible. At 30 min (A) adherent organisms appear as mono- and diplococci. By 60 min (B) tetracoccal and larger colonies are visible. Microcolonies are clearly visible along with new diplococcal and tetracoccal colonies. As colonies continue to grow (C), new microcolonies continue to be established (D). After 4 h of incubation, quite large colonies are visible adherent to or within the HEC-1-B cells (E).

Adherent gonococci form microcolonies on HEC-1-B cells.P+ Opa+ gonococci adhered to HEC-1-B cells as mono- and diplococci, as seen by light microscopy 30 min after inoculation (Fig. 1A). Cells that were infected usually had only one or two adherent mono- or diplococci, and these adherent organisms were not clustered (Fig. 1A). Adherent gonococci gradually formed microcolonies on the surfaces of the HEC-1-B cells during subsequent incubation (Fig.1B to E). After 1 h of incubation, colonies with four, six, and eight gonococci predominated, but colonies with higher numbers of organisms also were visible (Fig. 1B). By 90 min most colonies were too large for enumeration of individual cocci, but as the colonies continued to grow, new colonies with two and four gonococci could be seen (Fig. 1C and D). After 4 h of incubation, quite large colonies that had formed from the P+ Opa+inoculum could be seen adherent to or within HEC-1-B cells (Fig. 1E).

The CFU of adherent P+ Opa− organisms also increased between 30 min and 2 h of incubation. In contrast, there was no apparent increase in the CFU of adherent P−Opa− or P− Opa+ gonococci over incubation times of between 30 min and 7 h.

Adherent gonococci invade HEC-1-B cells by 4 h.Invasion of HEC-1-B cells paralleled adherence but was not saturable (Table 1). No more than 2% of the CFU that were associated with HEC-1-B cells after 4 h of incubation were within the cells, as judged by gonococcal survival in the presence of gentamicin. The proportion of associated CFU that was intracellular by 4 h was roughly constant for inocula of from 104 to 106 CFU/ml but was four times greater for the 107 inoculum than for the 106 inoculum. The number of intracellular FA1090 OpaE CFU was the same at 4 h as the number of MS11mkC CFU, even though 2 to 3 times fewer FA1090 OpaE variants than MS11mkC variants adhered.

Effects of piliation and Opa expression on gonococcal microcolony and HEC-1-B cell microvillus morphologies.Microcolonies formed by piliated gonococci appeared under SEM to be quite different from those formed by nonpiliated organisms. Figure2A shows SEM of a typical P−Opa− microcolony after 6 h of incubation on an HEC-1-B cell. P− Opa− colonies were loose, with moderate coccus-to-coccus adhesion. Microvilli of the HEC-1-B cells appeared to adhere only at their distal ends to individual cocci within the microcolony, and these microvilli were not distorted except by simple contiguity. Expression of Opa by nonpiliated gonococci (Fig.2B) did not change the appearance of their microcolonies appreciably and did not result in changes in the morphology of HEC-1-B cell microvilli or their interactions with the organisms.

Fig. 2.
  • Open in new tab
  • Download powerpoint
Fig. 2.

Gonococci must express both Opa and pili (P) in order to signal HEC-1-B cells to engulf them. (A and B) SEM photomicrographs of typical microcolonies of MS11mk P− organisms after 6 hours of growth on HEC-1-B cells. The bacteria in panel B also make Opa. Pili are not seen. (C and D) SEM photomicrographs of typical microcolonies of MS11mk P+ organisms. The bacteria in panel D also make Opa. Strands of pili are visible. (A) The P− Opa− microcolony is loose, with moderate bacterium-bacterium adhesion. Microvilli can be seen protruding from between individual gonococci, but there is little, if any, distortion of microvillus architecture other than that by simple contiguity. Some microvillus distal ends appear to have adhered to individual cocci. (B) The interaction between microvilli and the P−Opa+ microcolony is not different from that seen with P− Opa− organisms (panel A). Opa expression appears to have little influence on the reaction of the epithelial cells to the organisms. (C) Pili appear to “lash” individual P+ Opa− gonococci together to form a more compact colony. Some pili have bound microvilli together or pulled them toward the microcolony, modestly distorting microvillus architecture. Other strands of pili are visible radiating some distance from the microcolony along the surface of the epithelial cell. (D) The microvillus architecture has been altered dramatically as microvilli extend toward the microcolony of P+ Opa+organisms. The microcolony consists of tightly adherent organisms that are bound together. More peripheral microvilli have extended themselves toward the microcolony to extraordinary lengths. As highly elongated peripheral microvilli adhere to the colony, the microvilli appear to pull the HEC-1-B cell around the colony and lift the HEC-1-B cell from the coverslip. Magnification, ×8,000; bar, 2 μm.

Microcolonies formed by piliated gonococci were quite different. The cocci appeared to be bound together by their pili into compact colonies (Fig. 2C and D). Expression of Opa by piliated organisms resulted in even more compact microcolonies in which organisms were bound together by extensive coccus-coccus adhesions and webs of pili (Fig. 2D). Some pili of Opa− variants adhered to microvilli and occasionally seemed to be “pulling” them toward the microcolony, thereby modestly distorting the microvilli (Fig. 2C). The effect of Opa expression on the HEC-1-B cells, and in particular on the morphology of their microvilli, was most dramatic. The microvilli elongated toward the P+ Opa+ microcolonies and appeared to be pulling the HEC-1-B cell surface up around the gonococci (Fig. 2D). Some microvilli extended themselves to extraordinary lengths in order to surround P+ Opa+ cocci at the apex of the microcolony. This very active microvillus engagement of cocci within microcolonies was absent when the organisms made Opa but not pili (Fig.2B) or pili but not Opa (Fig. 2C).

The extent to which piliated Opa+ organisms activated HEC-1-B cell microvilli was clearer at higher magnifications (Fig.3). Microvilli adhered to nonpiliated Opa+ gonococci primarily at their distal ends (Fig. 3A), whereas they bound to piliated Opa+ gonococci along their full lengths (Fig. 3B). This caused elongated microvilli to wrap tightly around a considerable circumference of individual cocci. As microvilli elongated to engage the apex of the colony, they drew the cell surface up around it (Fig. 3B), and the colony appeared to sink into the underlying cell cytoplasm (Fig.4).

Fig. 3.
  • Open in new tab
  • Download powerpoint
Fig. 3.

HEC-1-B cell microvilli adhere to P+Opa+ gonococci along the full lengths of the microvilli. (A) At this SEM magnification it is clear that after 6 h of incubation only the distal ends of microvilli adhere to MS11mk P− Opa+ organisms. Microvilli have not been elongated. Both true diplococci and adherent individual cocci are visible. (B) In this SEM photomicrograph microvilli can be seen to have wrapped themselves tightly around MS11mk P+ Opa+ cocci in the microcolony after 6 h of incubation on HEC-1-B cells. Microvilli adhere tightly to individual cocci up to the full lengths of the microvilli. Microvillus adherence is clearly separate from bacterium-pilus interactions. Microvilli at the top of the micrograph appear to have pulled the cell surface up along the side of the colony, creating the appearance of a microcolony hanging from a pedestal of microvilli. (The electron beam is focused vertically, not horizontally.) The extent of microvillus elongation is remarkable, and the contrast between microvillar responses to piliated and nonpiliated Opa+ bacteria is striking. Magnification, ×15,500; bar, 1 μm.

Fig. 4.
  • Open in new tab
  • Download powerpoint
Fig. 4.

HEC-1-B cells engulf microcolonies with P+Opa+ gonococci. After 6 h of incubation, the MS11mk P+ Opa+ microcolonies appear to sink into the HEC-1-B cell in these SEM photomicrographs as activated microvilli pull the underlying cell surfaces up around the colony. Magnification, ×8,000; bar, 2 μm.

The requirement for gonococci to express both pili and Opa in order to activate microvilli was not unique to the MS11mk strain. HEC-1-B cellular responses to the pilus and Opa variants of strains F62-SF and FA1090 were the same as those to the MS11mkvariants. Only piliated Opa+ variants of F62-SF and FA1090 activated microvillus-directed engulfment.

Microvillus elongation did not begin before 2 h of incubation with P+ Opa+ organisms, with the exception of the piliated FA1090 Opa variant E (OpaE), and most of the P+Opa+ variants needed 4 to 6 h of incubation before microvilli elongated. The piliated FA1090 OpaE variant was unique in that it stimulated microvillus elongation within 1 h.

TEM was used to confirm that microvilli contributed to the internalization of piliated Opa+ organisms. Elongated microvilli adhered full length to P+ Opa+organisms (Fig. 5A and B), whereas microvilli were not elongated in the presence of P−Opa+ gonococci, and the very few microvilli that engaged the bacteria did so only at their distal ends (Fig. 5C and D). Some nonpiliated Opa+ organisms lay on the cell surface without perturbing it.

Fig. 5.
  • Open in new tab
  • Download powerpoint
Fig. 5.

TEM of P+ Opa+ organisms with activated microvilli adherent along their full lengths. (A and B) FA1090 P+ OpaE after 10 h of incubation on HEC-1-B cells. Microvilli have elongated and are adhering along their full lengths to gonococci, causing a perturbation of the HEC-1-B cell surface. Internalized cocci that are visible in both panels A and B appear to be free in the cell cytoplasm; vacuole membranes are not clearly visible (A). A bundle of pili is visible extending from the surface of a nonadherent coccus in panel B. (C and D) FA1090 P− OpaE after 10 h of incubation on HEC-1-B cells. Microvilli have not elongated, and the HEC-1-B cell surface is not perturbed. Some of these nonpiliated organisms lie in contiguity with the cell surface, but they are not being internalized. Some vacuoles within the cell cytoplasm appear to contain degenerating organisms. Bacterium-bacterium adhesion is apparent in both panels; tip adherence by microvilli also can be seen in both panels. Bar, 1 μm.

After 10 h of incubation, the HEC-1-B cells that had engulfed P+ Opa+ gonococci were filled with organisms that were not within vacuoles (Fig. 6A). At higher magnifications (Fig. 7), cell membranes could be seen to be surrounding engulfed P+Opa+ organisms, but the membrane fragments were closely apposed, or adherent, to the bacterial membranes, without any vacuolar space between them. Many of the internalized P+Opa+ gonococci maintained the close bacterium-bacterium adherence, and dividing cocci could be seen within the cell (Fig. 6A). Other HEC-1-B cells, often in sufficiently close contiguity to have formed tight junctions with infected HEC-1-B cells, contained no organisms (Fig. 6A), reflecting the very selective nature of invasion. Only an occasional P− Opa+ gonococcus was found within a cell, and they were clearly within vacuoles (Fig. 6B). Some vacuoles contained degenerated bacterial forms (Fig. 5D).

Fig. 6.
  • Open in new tab
  • Download powerpoint
Fig. 6.

Only P+ Opa+ organisms are found within HEC-1-B cells. TEM photomicrographs of HEC-1-B cells after 10 h of incubation with FA1090 P+ OpaE (A) and FA1090 P− OpaE (B) organisms are shown. (A) Two cells are closely apposed, appearing as a single, ovoid cell. (Note the two nuclei, the tight junctions at the periphery, and microvilli in the space below the upper nucleus.) The lower (and larger) of the two cells contains what appears to be an internalized microcolony in which the individual cocci remain tightly adherent to each other; cocci may be in the process of being internalized into the upper cell. Vacuole membranes are not apparent around the ingested organisms. (B) Two cells are closely apposed, appearing as a single, ovoid cell. (Note the two nuceli, the tight junctions at the periphery, and the microvilli in the space between the nuclei.) The lower (and smaller) of the two cells contains a single internalized coccus, visible at the lower right; it appears to be within a vacuole. Other vacuoles within the cytoplasm are empty. (The “vacuoles” between the nuclei are intercellular spaces.) A second coccus is in close contact with the cell membrane. Bar, 1 μm.

Fig. 7.
  • Open in new tab
  • Download powerpoint
Fig. 7.

High magnification (×50,000) of an intracellular FA1090 P+ OpaE organism that is in the process of dividing. Fragments of host cell membrane are visible in very close apposition to the bacterial outer membrane. This is most visible at the “waist” of the dividing coccus, but redundant fragments can be seen surrounding most of the organism. Note the absence of a clear vacuolar space between the host cell and bacterial cell membranes.

Effects of protein synthesis inhibitors.Treatment with 1 μg of chloramphenicol per ml, which would inhibit protein synthesis by the bacteria but not that by the eukaryotic cells, caused a diminution in microvillus elongation at 6 h and stopped engulfment of P+ Opa+ organisms (Fig.8). In the presence of chloramphenicol concentrations of ≥5 μg/ml (Fig. 8), Opa+ organisms no longer made abundant pili (Fig. 8), and microvillus elongation was abolished altogether. At chloramphenicol concentrations that would inhibit protein synthesis by the eukaryotic cells (≥25 μg/ml), the poorly piliated and loose bacterial colonies again appeared to sink into the underlying HEC-1-B cells, but this was not accompanied by the elongation of microvilli (Fig. 8).

Fig. 8.
  • Open in new tab
  • Download powerpoint
Fig. 8.

Chloramphenicol abolishes microvillus elongation and bacterial internalization at low doses but causes microvillus-independent HEC-1-B cell engulfment at higher doses. For these experiments FA1090 P+ OpaE variants were used, as these organisms stimulate microvillus elongation within 1 h. The HEC-1-B cells were incubated for 30 min with 0 to 50 μg of chloramphenicol per ml, the spent medium was aspirated, the HEC-1-B cells were inoculated with gonococci that had been diluted in medium containing the appropriate concentration of chloramphenicol, and the HEC-1-B cells were incubated for various times. As little as 5 μg of chloramphenicol per ml abolished microvillus elongation at 6 h; 10 μg of chloramphenicol per ml was needed to abolish microvillus elongation within the first hour. (A) FA1090 P+ OpaE after 6 h of incubation with HEC-1-B cells in the absence of chloramphenicol. (B to D) FA1090 P+ OpaE after 6 h of incubation with HEC-1-B cells in the presence of 1, 5, and 10 μg of chloramphenicol per ml. Note that microvillus-dependent engulfment appears to be lost at these concentrations of chloramphenicol. (E and F) FA1090 P+ OpaE after 6 h of incubation with HEC-1-B cells in the presence of 25 and 50 μg of chloramphenicol per ml. Note that the microcolonies are sinking into the HEC-1-B cells at these concentrations of chloramphenicol but that microvilli are not participating in their internalization. The magnification is given at the bottom of each panel.

These results suggested that the higher chloramphenicol concentrations caused the HEC-1-B cells to lose their ability to resist gonococcal invasion, whereas the lower concentrations primarily affected the bacteria. If so, then drugs that would affect only eukaryotic protein synthesis should enhance gonococcal invasion. We tested this hypothesis by repeating the experiments in the presence of increasing concentrations of anisomycin, an antibiotic that blocks peptidyl transferase activity on eukaryotic ribosomes, and cycloheximide, which blocks translocation on eukaryotic ribosomes. We used culture to quantitate the effects of the two drugs, as neither affected N. gonorrhoeae viability. Increasing the concentrations of both drugs increased gonococcal uptake by the human cells; anisomycin was the more potent on a molar basis (Fig. 9).

Fig. 9.
  • Open in new tab
  • Download powerpoint
Fig. 9.

Effect of eukaryotic cell protein synthesis inhibitors anisomycin and cyclohexamide on invasion of HEC-1-B cells by FA1090 P+ OpaE after 6 h of incubation. Means and standard errors for two or three experiments are shown.

Effects of cytoskeleton inhibitors.Treatment of HEC-1-B cells with cytochalasins B and D, which block actin polymerization and paralyze cell movement, stopped gonococcal uptake in a dose-dependent way (data not shown). Cytochalasin D was 10-fold more active (50% inhibitory doses of 50 versus 500 ng). Treatment with colchicine, which binds tubulin and prevents its polymerization, did not affect gonococcal uptake, even at concentrations of 50 μM (data not shown). None of these drugs affected the viability of gonococci or their ability to adhere to the human cells.

DISCUSSION

The data presented in this paper strongly support the conclusion that pili and Opa function coordinately in N. gonorrhoeaeadherence to and invasion of HEC-1-B cells. The gonococci that lacked pili or Opa or both did not demonstrate the same active interaction with the HEC-1-B cells as the Opa+ P+gonococci.

Initial adherence of the Opa+ P+ gonococci was to the distal ends of microvilli of only a few HEC-1-B cells. Adherence was saturable and was to only a subpopulation of cells, as initially no gonococci were visibly attached to a majority of the cells, also suggesting that the ligand that mediates adherence was not present on many cells. Opa− and Opa+ gonococci both adhered to the distal ends of the microvilli. The adherence of the Opa− gonococci suggests that adhesins other than Opa mediate the initial close attachment of the gonococci to the microvilli (4, 14, 36, 39, 59, 74). Several studies have identified lectin-like adhesins other than pili and Opa that bind human glycosphingolipid structures (15, 47, 50, 51, 62). Gonococcal adherence to the distal ends of microvilli in the fallopian tube organ culture model was noted previously (20).

Initially the adherent Opa+ P+ gonococci were single cocci or diplococci. Over the next 30 min to few hours, the single cocci and diplococci became microcolonies. These appeared to be similar to the clusters of gonococci described by Novotny and coworkers after light microscopic and EM examination of pus from infected men and women; these clusters were termed infectious units (45, 46). The microcolonies of gonococci might be more representative of naturally occurring gonococcal infection than the single coccus or diplococcus.

The pili adhered to epithelial cell surfaces and microvilli, as seen by SEM, and also formed webs that bound the gonococci into compact colonies. The overall effect seemed to be to anchor these compact microcolonies onto the cell surface; similar observations have been made previously (16, 17).

As the microcolonies of Opa+ P+ gonococci developed, the HEC-1-B cell microvilli elongated and bound to the gonococci along much of their full lengths. Microvillus elongation and attachment to gonococci along the length of the microvilli in the fallopian tube organ culture model was noted previously (19). The microvilli appeared to be actively engaging the gonococci. The interaction of the microvilli with the gonococci that lacked pili or Opa or both was much different. Although pili were required for HEC-1-B cell microvillus activation and elongation, the pili were not sufficient; Opa also was required.

There have been previous reports that Opa expression by gonococci was required for invasion of various epithelial cell lines in vitro (39, 59, 74). Kupsch et al. found that Opa expression alone was not sufficient to support gonococcal invasion of HEC-1-B cells (36). The data in the present paper show that Opa expression was required for invasion, but, as with pili, it was not sufficient to direct engulfment by HEC-1-B cells; pili also were required.

The studies with experimental gonococcal infections of human volunteers have demonstrated that pili were essential for infection to occur (32, 33, 69). When the volunteers were inoculated with Opa− gonococci, there was a lag period during which the subjects had no symptoms (31, 56, 66). The gonococci cultured from the urine sediment shortly after inoculation tended to be the Opa− variants that were in the initial inoculum. Subsequently, the subjects developed symptoms and the gonococci present in the urinary sediments were uniformly Opa+. It is likely that the Opa− gonococci in the inoculum either were shed and excreted in the urine or shifted to Opa+ and proceeded to invade the urethral mucosa and cause symptomatic disease. The presence of Opa in the gonococci from the symptomatic volunteers strongly suggested that Opa, along with pili, was required for the gonococci to cause disease. This in vivo demonstration of the coordinate use of pili and Opa in initiating gonococcal infection was fully consistent with the observations in the present in vitro study, where pili and Opa coordinately promoted epithelial cell invasion.

Microscopy of naturally occurring infections shows viable, dividing gonococci, which do not appear to be within vacuoles, inside epithelial cells and polymorphonuclear leukocytes (24, 46, 71, 72). Internalized P+ Opa+ organisms looked like those seen during natural infection (24, 46, 71, 72). In contrast, the few P− Opa+ bacteria that were ingested appeared to be degenerate and within clearly visible parasitophorous vacuoles.

Chen et al. reported that preincubation of gonococcal strain F62 with glutaraldehyde-fixed HEC-1-B cells enabled the bacteria then to invade viable cells without the normal 6- to 8-h lag (10). Preincubation with the fixed cells did not make the organisms more adherent, but for invasion to be enhanced, the gonococci had to have contact with the fixed membranes of the HEC-1-B cells (10). These observations indicate that HEC-1-B cells have on their surfaces a molecule(s) that can signal adherent gonococci to begin synthesizing molecules that then promote active endocytosis by the HEC-1-B cells.

The OpaE variant of strain FA1090 differed from the other Opa+ strains in that it caused microvillus elongation in only 1 h. It therefore must have expressed constitutively the necessary ligand(s) that was induced in the other Opa variants and strains of gonococci.

Treatment with doses of chloramphenicol that interfered with bacterial but not eukaryotic protein synthesis abolished both intimate attachment to microvilli and gonococcal invasion. This is consistent with the notion that protein synthesis by the gonococcus yielded a ligand that engaged proximal microvillus receptors. Chen et al. also reported that chloramphenicol treatment during preincubation abolished enhancement of invasion (10).

Drugs that blocked eukaryotic protein synthesis caused enhanced gonococcal invasion. At doses of chloramphenicol that interrupted eukaryotic protein synthesis, gonococcal microcolonies entered HEC-1-B cells without engaging microvilli. This same passive process may have accounted for the enhanced invasion that accompanied treatment of the eukaryotic cells with anisomycin and cyclohexamide. The HEC-1-B cells may naturally resist invasion. Activation of microvillus-mediated engulfment, then, can be seen as a strategy for circumventing natural resistance to invasion (6).

The finding that cytochalasins inhibited gonococcal invasion of various epithelial cell lines has been reported previously (3, 10, 58). Richardson and Sadoff found that colchicine also inhibited gonococcal invasion (52), but this drug did not inhibit uptake of FA1090 OpaE organisms by HEC-1-B cells in the present study. The discrepancy can explained by Richardson and Sadoff’s use of hamster kidney cells, rather than human epithelial cells. As N. gonorrhoeae is an obligate pathogen of human epithelial cells and polymorphonuclear leukocytes, its entry into rodent kidney cells could be expected to involve mechanisms different from those used to invade human cells. In addition, Richardson and Sadoff used different gonococcal strains.

ACKNOWLEDGMENTS

This work was supported by National Institutes of Health grants AI 21620 (to J.M.G.) and PO1 AI 21912 (to G.F.B.) and by the Research Service of the Department of Veterans Affairs (to J.M.G.).

May Fong helped administer the grants and prepared the manuscript, for which we thank her.

Notes

Editor: D. L. Burns

FOOTNOTES

    • Received 7 December 1998.
    • Returned for modification 29 January 1999.
    • Accepted 6 April 1999.
  • Copyright © 1999 American Society for Microbiology

REFERENCES

  1. 1.↵
    1. Backman M.,
    2. Källström H.,
    3. Jonsson A.-B.
    The phase-variable pilus-associated protein PilC is commonly expressed in clinical isolates of Neisseria gonorrhoeae, and shows sequence variability among strains.Microbiology1441998149156
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Barritt D. S.,
    2. Schwalbe R. S.,
    3. Klapper D. G.,
    4. Cannon J. G.
    Antigenic and structural differences among six proteins II expressed by a single strain of Neisseria gonorrhoeae.Infect. Immun.55198720262031
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Bessen D.,
    2. Gotschlich E. C.
    Interactions of gonococci with HeLa cells: attachment, detachment, replication, penetration, and the role of protein II.Infect. Immun.541986154160
    OpenUrlAbstract/FREE Full Text
  4. 4.↵
    1. Bhat K. S.,
    2. Gibbs C. P.,
    3. Barrera O.,
    4. Morrison S. G.,
    5. Jähnig F.,
    6. Stern A.,
    7. Kupsch E. M.,
    8. Meyer T. F.,
    9. Swanson J.
    The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes.Mol. Microbiol.5199118891901
    OpenUrlCrossRefPubMedWeb of Science
  5. 5.↵
    1. Black W. J.,
    2. Schwalbe R. S.,
    3. Nachamkin I.,
    4. Cannon J. G.
    Characterization of Neisseria gonorrhoeae protein II phase variation by use of monoclonal antibodies.Infect. Immun.451984453457
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Bliska J. B.,
    2. Galán J. E.,
    3. Falkow S.
    Signal transduction in the mammalian cell during bacterial attachment and entry.Cell.731993903920
    OpenUrlCrossRefPubMedWeb of Science
  7. 7.↵
    1. Bos M. P.,
    2. Grunert F.,
    3. Belland R. J.
    Differential recognition of members of the carcinoembryonic antigen family by Opa variants of Neisseria gonorrhoeae.Infect. Immun.65199723532361
    OpenUrlAbstract/FREE Full Text
  8. 8.↵
    1. Brooks G. F.,
    2. Olinger L.,
    3. Lammel C. J.,
    4. Bhat K. S.,
    5. Calvello C. A.,
    6. Palmer M. L.,
    7. Knapp J. S.,
    8. Stephens R. S.
    Prevalence of gene sequences coding for hypervariable regions of Opa (protein II) in Neisseria gonorrhoeae.Mol. Microbiol.5199130633072
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Burnette W. N.
    “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.Anal. Biochem.1121981195203
    OpenUrlCrossRefPubMedWeb of Science
  10. 10.↵
    1. Chen J. C.-R.,
    2. Bavoil P.,
    3. Clark V. L.
    Enhancement of the invasive ability of Neisseria gonorrhoeae by contact with HecIB, an adenocarcinoma endometrial cell line.Mol. Microbiol.5199115311538
    OpenUrlCrossRefPubMed
  11. 11.↵
    1. Chen T.,
    2. Belland R. J.,
    3. Wilson J.,
    4. Swanson J.
    Adherence of pilus− Opa+ gonococci to epithelial cells in vitro involves heparan sulfate.J. Exp. Med.1821995511517
    OpenUrlAbstract/FREE Full Text
  12. 12.↵
    1. Chen T.,
    2. Gotschlich E. C.
    CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins.Proc. Natl. Acad. Sci. USA9319961485114856
    OpenUrlAbstract/FREE Full Text
  13. 13.↵
    1. Chen T.,
    2. Grunert F.,
    3. Medina-Marino A.,
    4. Gotschlich E. C.
    Several carcinoembryonic antigens (CD66) serve as receptors for gonococcal opacity proteins.J. Exp. Med.185199715571564
    OpenUrlAbstract/FREE Full Text
  14. 14.↵
    1. Connell T. D.,
    2. Shaffer D.,
    3. Cannon J. G.
    Characterization of the repertoire of hypervariable regions in the protein II (opa) gene family of Neisseria gonorrhoeae.Mol. Microbiol.41990439449
    OpenUrlCrossRefPubMed
  15. 15.↵
    1. Deal C. D.,
    2. Krivan H. C.
    Lacto- and ganglio-series glycolipids are adhesion receptors for Neisseria gonorrhoeae.J. Biol. Chem.26519901277412777
    OpenUrlAbstract/FREE Full Text
  16. 16.↵
    1. Dekker N. P.,
    2. Lammel C. J.,
    3. Brooks G. F.
    Scanning electron microscopy of piliated Neisseria gonorrhoeae processed with hexamethyldisilazane.J. Elect. Microsc. Tech.191991461467
    OpenUrlCrossRefPubMedWeb of Science
  17. 17.↵
    1. Dekker N. P.,
    2. Lammel C. J.,
    3. Mandrell R. E.,
    4. Brooks G. F.
    Opa (protein II) influences gonococcal organization in colonies, surface appearance, size and attachment to human fallopian tube tissues.Microb. Pathog.919901931
    OpenUrlCrossRefPubMed
  18. 18.↵
    1. Densen P.,
    2. Rein M. F.,
    3. Sullivan J. A.,
    4. Mandell G. L.
    Morphological observations of neutrophil-gonococcus interaction Immunobiology of Neisseria gonorrhoeae. Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. 1978 213 220 American Society for Microbiology Washington, D.C
  19. 19.↵
    1. Draper D. L.,
    2. Donegan E. A.,
    3. James J. F.,
    4. Sweet R. L.,
    5. Brooks G. F.
    Scanning electron microscopy of attachment of Neisseria gonorrhoeae colony phenotypes to surfaces of human genital epithelia.Am. J. Obstet. Gynecol.1381980818826
    OpenUrlPubMed
  20. 20.↵
    1. Draper D. L.,
    2. Donegan E. A.,
    3. James J. F.,
    4. Sweet R. L.,
    5. Brooks G. F.
    In vitro modeling of acute salpingitis caused by Neisseria gonorrhoeae.Am. J. Obstet. Gynecol.13819809961002
    OpenUrlCrossRefPubMed
  21. 21.↵
    1. Draper D. L.,
    2. James J. F.,
    3. Brooks G. F.,
    4. Sweet R. L.
    Comparison of virulence markers of peritoneal and fallopian tube isolates with endocervical Neisseria gonorrhoeae isolates from women with acute salpingitis.Infect. Immun.271980882888
    OpenUrlAbstract/FREE Full Text
  22. 22.↵
    1. Gibbs D. L.,
    2. Roberts R. B.
    The interaction in vitro between human polymorphonuclear leukocytes and Neisseria gonorrhoeae cultivated in the chick embryo.J. Exp. Med.1411975155171
    OpenUrlAbstract/FREE Full Text
  23. 23.↵
    1. Grassmé H. U. C.,
    2. Ireland R. M.,
    3. van Putten J. P. M.
    Gonococcal opacity protein promotes bacterial entry-associated rearrangements of the epithelial cell actin cytoskeleton.Infect. Immun.64199616211630
    OpenUrlAbstract/FREE Full Text
  24. 24.↵
    1. Harkness A. H.
    The pathology of gonorrhoea.Br. J. Vener. Dis.241948137147
    OpenUrlPubMed
  25. 25.↵
    1. Harvey H. A.,
    2. Ketterer M. R.,
    3. Preston A.,
    4. Lubaroff D.,
    5. Williams R.,
    6. Apicella M. A.
    Ultrastructural analysis of primary human urethral epithelial cell cultures infected with Neisseria gonorrhoeae.Infect. Immun.65199724202427
    OpenUrlAbstract/FREE Full Text
  26. 26.↵
    1. Ilver D.,
    2. Källström H.,
    3. Normark S.,
    4. Jonsson A.-B.
    Transcellular passage of Neisseria gonorrhoeae involves pilus phase variation.Infect. Immun.661998469473
    OpenUrlAbstract/FREE Full Text
  27. 27.↵
    1. Ison C. A.,
    2. Crabtree H. L.,
    3. Lammel C. J.,
    4. Brooks G. F.
    Expression of protein II by clinical isolates of Neisseria gonorrhoeae Neisseriae 1990. Achtman M., Kohl P., Marchal C., Morelli G., Seiler A., Thiesen B. 1991 597 602 Walter de Gruyter New York, N.Y
  28. 28.↵
    1. James J. F.,
    2. Swanson J.
    Piliation of gonococci in vivo.J. Infect. Dis.13719789495
    OpenUrlCrossRefPubMed
  29. 29.↵
    1. James J. F.,
    2. Swanson J.
    Color/opacity colonial variants of Neisseria gonorrhoeae and their relationship to the menstrual cycle Immunobiology of Neisseria gonorrhoeae. Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. 1978 338 343 American Society for Microbiology Washington, D.C
  30. 30.↵
    1. Jephcott A. E.,
    2. Reyn A.,
    3. Birch-Andersen A.
    Neisseria gonorrhoeae. III. Demonstration of presumed appendages to cells from different colony types.Acta Pathol. Microbiol. Scand. Sect. B791971437439
    OpenUrl
  31. 31.↵
    1. Jerse A. E.,
    2. Cohen M. S.,
    3. Drown P. M.,
    4. Wicker L. G.,
    5. Isbey S. F.,
    6. Seifert H. S.,
    7. Cannon J. G.
    Multiple gonococcal opacity proteins are expressed during experimental urethral infection in the male.J. Exp. Med.1791994911920
    OpenUrlAbstract/FREE Full Text
  32. 32.↵
    1. Kellogg D. S. Jr.,
    2. Peacock W. L. Jr.,
    3. Deacon W. E.,
    4. Brown L.,
    5. Pirkle C. I.
    Neisseria gonorrhoeae. I. Virulence genetically linked to clonal variation.J. Bacteriol.85196312741279
    OpenUrlAbstract/FREE Full Text
  33. 33.↵
    1. Kellogg D. S. Jr.,
    2. Cohen I. R.,
    3. Norins L. C.,
    4. Schroeter A. L.,
    5. Reising G.
    Neisseria gonorrhoeae. II. Colonial variatioin and pathogenicity during 35 months in vitro.J. Bacteriol.961968596605
    OpenUrlAbstract/FREE Full Text
  34. 34.↵
    1. King G. J.,
    2. Swanson J.
    Studies on gonococcus infection. XV. Identification of surface proteins of Neisseria gonorrhoeae correlated with leukocyte association.Infect. Immun.211978575584
    OpenUrlAbstract/FREE Full Text
  35. 35.↵
    1. Kovalchik M. T.,
    2. Kraus S. J.
    Neisseria gonorrhoeae: colonial morphology of rectal isolates.Appl. Microbiol.231972986989
    OpenUrlPubMed
  36. 36.↵
    1. Kupsch E. M.,
    2. Knepper B.,
    3. Kuroki T.,
    4. Heuer I.,
    5. Meyer T. F.
    Variable opacity (Opa) outer membrane proteins account for the cell tropisms displayed by Neisseria gonorrhoeae for human leukocytes and epithelial cells.EMBO J.121993641650
    OpenUrlPubMedWeb of Science
  37. 37.↵
    1. Laemmli U. K.
    Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature (London)2271970680685
    OpenUrlCrossRefPubMedWeb of Science
  38. 38.↵
    1. Lammel C. J.,
    2. Karu A. E.,
    3. Brooks G. F.
    Antigenic specificity and biological activity of a monoclonal antibody that is broadly cross reactive with gonococcal protein IIs Gonococci and meningococci. Poolman J. T., Zanen H. C., Mayer T. F., Heckels J. E., Makela P. R. H., Smith H., Beuvery E. C. 1988 737 743 Kluwer Academic Publishers Dordrecht, The Netherlands
  39. 39.↵
    1. Makino S.,
    2. van Putten J. P. M.,
    3. Meyer T. F.
    Phase variation of the opacity outer membrane protein controls invasion by Neisseria gonorrhoeae into human epithelial cells.EMBO J.10199113071315
    OpenUrlPubMedWeb of Science
  40. 40.↵
    1. Mayer L. W.
    Rates of in vitro changes of gonococcal colony opacity phenotypes.Infect. Immun.371982481485
    OpenUrlAbstract/FREE Full Text
  41. 41.
    1. McGee Z. A.,
    2. Stephens D. S.,
    3. Hoffman L. H.,
    4. Schlech W. F. III,
    5. Horn R. G.
    Mechanisms of mucosal invasion by pathogenic Neisseria.Rev. Infect. Dis.51983S708S714
    OpenUrlCrossRefPubMed
  42. 42.↵
    1. McGee Z. A.,
    2. Johnson A. P.,
    3. Taylor-Robinson D.
    Pathogenic mechanisms of Neisseria gonorrhoeae: observations on damage to human fallopian tubes in organ culture by gonococci of colony type 1 or type 4.J. Infect. Dis.1431981413422
    OpenUrlCrossRefPubMedWeb of Science
  43. 43.↵
    1. Mosleh I. M.,
    2. Boxberger H.-J.,
    3. Sessler M. J.,
    4. Meyer T. F.
    Experimental infection of native human ureteral tissue with Neisseria gonorrhoeae: adhesion, invasion, intracellular fate, exocytosis and passage through a stratified epithelium.Infect. Immun.65199733913398
    OpenUrlAbstract/FREE Full Text
  44. 44.↵
    1. Murphy G. L.,
    2. Connell T. D.,
    3. Barritt D. S.,
    4. Koomey M.,
    5. Cannon J. G.
    Phase variation of gonococcal protein II: regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence.Cell561989539547
    OpenUrlCrossRefPubMedWeb of Science
  45. 45.↵
    1. Novotny P.,
    2. Short J. A.
    The role of infectious units in the pathogenicity of Neisseria gonorrhoeae Gonorrhoea: epidemiology and pathogenesis. Skinner F. A., Walker P. D., Smith H. 1977 185 205 Academic Press London, United Kingdom
  46. 46.↵
    1. Novotny P.,
    2. Short J. A.,
    3. Walker P. D.
    An electron-microscope study of naturally occurring and cultured cells of Neisseria gonorrhoeae.J. Gen. Microbiol.81975413427
    OpenUrl
  47. 47.↵
    1. Nyberg G.,
    2. Strömberg N.,
    3. Jonsson A.,
    4. Karlsson K.-A.,
    5. Normark S.
    Erythrocyte gangliosides act as receptors for Neisseria subflava: identification of the Sia-1 adhesin.Infect. Immun.58199025552563
    OpenUrlAbstract/FREE Full Text
  48. 48.↵
    1. Ofek I.,
    2. Beachy E. H.,
    3. Bisno A. L.
    Resistance of Neisseria gonorrhoeae to phagocytosis: relationship to colonial morphology and surface pili.J. Infect. Dis.1291974310316
    OpenUrlCrossRefPubMed
  49. 49.
    1. Paruchuri D. K.,
    2. Seifert H. S.,
    3. Ajioka R. S.,
    4. Karlsson K.-A.,
    5. So M.
    Identification and characterization of a Neisseria gonorrhoeae gene encoding a glycolipid-binding adhesin.Proc. Natl. Acad. Sci. USA871990333337
    OpenUrlAbstract/FREE Full Text
  50. 50.↵
    1. Porat N.,
    2. Apicella M. A.,
    3. Blake M. S.
    A lipooligosaccharide-binding site on HepG2 cells similar to the gonococcal opacity-associated surface protein Opa.Infect. Immun.63199521642172
    OpenUrlAbstract/FREE Full Text
  51. 51.↵
    1. Rest R. F.,
    2. Lee N.,
    3. Bowden C.
    Stimulation of human leukocytes by protein II+ gonococci is mediated by lectin-like gonococcal components.Infect. Immun.501985116122
    OpenUrlAbstract/FREE Full Text
  52. 52.↵
    1. Richardson W. P.,
    2. Sadoff J. C.
    Induced engulfment of Neisseria gonorrhoeae by tissue culture cells.Infect. Immun.56198825122514
    OpenUrlAbstract/FREE Full Text
  53. 53.↵
    1. Rudel T.,
    2. Boxberger H.-J.,
    3. Meyer T. F.
    Pilus biogenesis and epithelial cell adherence of Neisseria gonorrhoeae pilC double knock-out mutants.Mol. Microbiol.17199510571071
    OpenUrlCrossRefPubMedWeb of Science
  54. 54.↵
    1. Rudel T.,
    2. van Putten J. P. M.,
    3. Gibbs C. P.,
    4. Haas R.,
    5. Meyer T. F.
    Interaction of two variable proteins (PilE and PilC) required for pilus-mediated adherence of Neisseria gonorrhoeae to human epithelial cells.Mol. Microbiol.6199234393450
    OpenUrlCrossRefPubMedWeb of Science
  55. 55.↵
    1. Ryll R. R.,
    2. Rudel T.,
    3. Scheuerpflug I.,
    4. Barten R.,
    5. Meyer T. F.
    PilC of Neisseria meningitidis is involved in class II pilus formation and restores pilus assembly, natural transformation competence and adherence to epithelial cells in PilC-deficient gonococci.Mol. Microbiol.231997879892
    OpenUrlCrossRefPubMed
  56. 56.↵
    1. Schneider H.,
    2. Cross A. S.,
    3. Kuschner R. A.,
    4. Taylor D. N.,
    5. Sadoff J. C.,
    6. Boslego J. W.,
    7. Deal C. D.
    Experimental human gonococcal urethritis: 250 Neisseria gonorrhoeae MS11mkC are infective.J. Infect. Dis.1721995180185
    OpenUrlCrossRefPubMedWeb of Science
  57. 57.↵
    1. Schneider H.,
    2. Griffiss J. M.,
    3. Boslego J. W.,
    4. Hitchcock P. J.,
    5. Zahos K. M.,
    6. Apicella M. A.
    Expression of paragloboside-like lipooligosaccharides may be a necessary component of gonococcal pathogenesis in men.J. Exp. Med.174199116011605
    OpenUrlAbstract/FREE Full Text
  58. 58.↵
    1. Shaw J. H.,
    2. Falkow S.
    Model for invasion of human tissue culture cells by Neisseria gonorrhoeae.Infect. Immun.56198816251632
    OpenUrlAbstract/FREE Full Text
  59. 59.↵
    1. Simon D.,
    2. Rest R. F.
    Escherichia coli expressing a Neisseria gonorrhoeae opacity-associated outer membrane protein invade human cervical and endometrial epithelial cell lines.Proc. Natl. Acad. Sci. USA89199255125516
    OpenUrlAbstract/FREE Full Text
  60. 60.↵
    1. Sparling P. F.,
    2. Yobs A. R.
    Colonial morphology of Neisseria gonorrhoeae isolated from males and females.J. Bacteriol.931967513
    OpenUrlFREE Full Text
  61. 61.↵
    1. Stern A.,
    2. Brown M.,
    3. Nickel P.,
    4. Meyer T. F.
    Opacity genes in Neisseria gonorrhoeae: control of phase and antigenic variation.Cell4719866171
    OpenUrlCrossRefPubMedWeb of Science
  62. 62.↵
    1. Strömberg N.,
    2. Deal C.,
    3. Nyberg G.,
    4. Normark S.,
    5. So M.,
    6. Karlsson K.-A.
    Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae.Proc. Natl. Acad. Sci. USA85198849024906
    OpenUrlAbstract/FREE Full Text
  63. 63.↵
    1. Swanson J.
    Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells.J. Exp. Med.1371973571589
    OpenUrlAbstract
  64. 64.↵
    1. Swanson J.
    Studies on gonococcus infection. XII. Colony color and opacity variants of gonococci.Infect. Immun.191978320331
    OpenUrlAbstract/FREE Full Text
  65. 65.↵
    1. Swanson J.
    Studies on gonococcus infection. XIV. Cell wall protein differences among color/opacity colony variants of Neisseria gonorrhoeae.Infect. Immun.211978292302
    OpenUrlAbstract/FREE Full Text
  66. 66.↵
    1. Swanson J.,
    2. Barrera O.,
    3. Sola J.,
    4. Boslego J.
    Expression of outer membrane protein II by gonococci in experimental gonorrhea.J. Exp. Med.168198821212129
    OpenUrlAbstract/FREE Full Text
  67. 67.↵
    1. Swanson J.,
    2. Bergström S.,
    3. Robbins K.,
    4. Barrera O.,
    5. Corwin D.,
    6. Koomey J. M.
    Gene conversion involving the pilin structural gene correlates with pilus+ pilus− changes in Neisseria gonorrhoeae.Cell471986267276
    OpenUrlCrossRefPubMedWeb of Science
  68. 68.↵
    1. Swanson J.,
    2. Kraus S. J.,
    3. Gotschlich E. C.
    Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns.J. Exp. Med.1341971886906
    OpenUrlAbstract
  69. 69.↵
    1. Swanson J.,
    2. Robbins K.,
    3. Barrera O.,
    4. Corwin D.,
    5. Boslego J.,
    6. Ciak J.,
    7. Blake M.,
    8. Koomey J. M.
    Gonococcal pilin variants in experimental gonorrhea.J. Exp. Med.165198713441357
    OpenUrlAbstract/FREE Full Text
  70. 70.↵
    1. Thompson J. A.,
    2. Grunert F.,
    3. Zimmermann W.
    Carcinoembryonic antigen gene family: molecular biology and clinical perspectives.J. Clin. Lab. Anal.51991344366
    OpenUrlCrossRefPubMedWeb of Science
  71. 71.↵
    1. Ward M. E.,
    2. Glynn A. A.,
    3. Watt P. J.
    The fate of gonococci in polymorphonuclear leukocytes: an electron-microscopic study of the natural disease.Br. J. Exp. Pathol.531972289294
    OpenUrlPubMed
  72. 72.↵
    1. Ward M. E.,
    2. Watt P. J.
    Adherence of Neisseria gonorrhoeae to urethral mucosal cells: an electron-microscopic study of human gonorrhea.J. Infect. Dis.1261972601605
    OpenUrlCrossRefPubMedWeb of Science
  73. 73.↵
    1. Ward M. E.,
    2. Watt P. J.,
    3. Robertson J. N.
    The human fallopian tube: a laboratory model for gonococcal infection.J. Infect. Dis.1291974650659
    OpenUrlCrossRefPubMedWeb of Science
  74. 74.↵
    1. Weel J. F. L.,
    2. Hopman C. T. P.,
    3. van Putten J. P. M.
    In situ expression and localization of Neisseria gonorrhoeae opacity proteins in infected epithelial cells: apparent role of Opa proteins in cellular invasion.J. Exp. Med.173199113951405
    OpenUrlAbstract/FREE Full Text
View Abstract
PreviousNext
Back to top
Download PDF
Citation Tools
Neisseria gonorrhoeae Coordinately Uses Pili and Opa To Activate HEC-1-B Cell Microvilli, Which Causes Engulfment of the Gonococci
J. McLeod Griffiss, Claudia J. Lammel, Jun Wang, Nusi P. Dekker, G. F. Brooks
Infection and Immunity Jul 1999, 67 (7) 3469-3480; DOI: 10.1128/IAI.67.7.3469-3480.1999

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Infection and Immunity article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Neisseria gonorrhoeae Coordinately Uses Pili and Opa To Activate HEC-1-B Cell Microvilli, Which Causes Engulfment of the Gonococci
(Your Name) has forwarded a page to you from Infection and Immunity
(Your Name) thought you would be interested in this article in Infection and Immunity.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Neisseria gonorrhoeae Coordinately Uses Pili and Opa To Activate HEC-1-B Cell Microvilli, Which Causes Engulfment of the Gonococci
J. McLeod Griffiss, Claudia J. Lammel, Jun Wang, Nusi P. Dekker, G. F. Brooks
Infection and Immunity Jul 1999, 67 (7) 3469-3480; DOI: 10.1128/IAI.67.7.3469-3480.1999
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • Notes
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Antigens, Bacterial
Bacterial Adhesion
Endometrium
Fimbriae, Bacterial
Microvilli
Neisseria gonorrhoeae

Related Articles

Cited By...

About

  • About IAI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #IAIjournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0019-9567; Online ISSN: 1098-5522