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Microbial Immunity and Vaccines

Early Induction of Gamma Interferon and Interleukin-10 Production in Draining Lymph Nodes from Mice Infected withBorrelia burgdorferi

Frederic Ganapamo, Vida A. Dennis, Mario T. Philipp
Frederic Ganapamo
Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana 70433
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Vida A. Dennis
Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana 70433
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Mario T. Philipp
Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana 70433
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DOI: 10.1128/IAI.68.12.7162-7165.2000
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    Fig. 1.

    In vitro production of IFN-γ and IL-10 by LN cells from C3H/HeJ and C57BL/6J mice after 1 week of infection with the JD1 strain of B. burgdorferi (Bb). LN cells from naive mice were used as a control. Cells (2 × 106/ml) were stimulated in vitro with freeze-thawed JD1 spirochetes (1 × 107/ml) or ConA (10 μg/ml). The control supernatant was generated by culturing cells in medium alone. Supernatants were harvested at 24 h (ConA) or 48 h (B. burgdorferi). A sandwich ELISA was performed for cytokine detection. Student'st test was used for statistical analysis. ∗ and ∗∗, P < 0.006 and P < 0.04, respectively. These experiments were conducted in triplicate and repeated four times, with similar results.

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    Fig. 2.

    Differential effect of in vitro neutralization of IFN-γ and IL-10 on their production by draining LN cells from C3H/HeJ and C57BL/6J mice 1 week p.i. Freshly isolated LN cells (2 × 106/ml) were stimulated in vitro with freeze-thawedB. burgdorferi (Bb) JD1 spirochetes (1 × 107/ml). Neutralizing MAb anti-IL-10 or anti-IFN-γ (25 μg/ml) was added in some of the wells. The control supernatant was generated by culturing cells in medium alone. Supernatants were collected 48 h later and a sandwich ELISA was performed for cytokine detection. Student's t test was used for statistical analysis. ∗ and ∗∗, P < 0.00003 and P < 0.0015, respectively. All samples were run in quadruplicate. Data are representative of four separate experiments.

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Early Induction of Gamma Interferon and Interleukin-10 Production in Draining Lymph Nodes from Mice Infected withBorrelia burgdorferi
Frederic Ganapamo, Vida A. Dennis, Mario T. Philipp
Infection and Immunity Dec 2000, 68 (12) 7162-7165; DOI: 10.1128/IAI.68.12.7162-7165.2000

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Early Induction of Gamma Interferon and Interleukin-10 Production in Draining Lymph Nodes from Mice Infected withBorrelia burgdorferi
Frederic Ganapamo, Vida A. Dennis, Mario T. Philipp
Infection and Immunity Dec 2000, 68 (12) 7162-7165; DOI: 10.1128/IAI.68.12.7162-7165.2000
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KEYWORDS

Interferon-gamma
interleukin-10
Lyme disease
Lymph Nodes

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