Article Figures & Data
Figures
- Fig. 1.
In vitro production of IFN-γ and IL-10 by LN cells from C3H/HeJ and C57BL/6J mice after 1 week of infection with the JD1 strain of B. burgdorferi (Bb). LN cells from naive mice were used as a control. Cells (2 × 106/ml) were stimulated in vitro with freeze-thawed JD1 spirochetes (1 × 107/ml) or ConA (10 μg/ml). The control supernatant was generated by culturing cells in medium alone. Supernatants were harvested at 24 h (ConA) or 48 h (B. burgdorferi). A sandwich ELISA was performed for cytokine detection. Student'st test was used for statistical analysis. ∗ and ∗∗, P < 0.006 and P < 0.04, respectively. These experiments were conducted in triplicate and repeated four times, with similar results.
- Fig. 2.
Differential effect of in vitro neutralization of IFN-γ and IL-10 on their production by draining LN cells from C3H/HeJ and C57BL/6J mice 1 week p.i. Freshly isolated LN cells (2 × 106/ml) were stimulated in vitro with freeze-thawedB. burgdorferi (Bb) JD1 spirochetes (1 × 107/ml). Neutralizing MAb anti-IL-10 or anti-IFN-γ (25 μg/ml) was added in some of the wells. The control supernatant was generated by culturing cells in medium alone. Supernatants were collected 48 h later and a sandwich ELISA was performed for cytokine detection. Student's t test was used for statistical analysis. ∗ and ∗∗, P < 0.00003 and P < 0.0015, respectively. All samples were run in quadruplicate. Data are representative of four separate experiments.
