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Bacterial Infections

Interaction between Burkholderia pseudomallei and Acanthamoeba Species Results in Coiling Phagocytosis, Endamebic Bacterial Survival, and Escape

Timothy J. J. Inglis, Paul Rigby, Terry A. Robertson, Nichole S. Dutton, Mandy Henderson, Barbara J. Chang
Timothy J. J. Inglis
Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, Western Australia 6009 and
Department of Microbiology,
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Paul Rigby
Biomedical Confocal Microscopy Research Centre, Department of Pharmacology, and
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Terry A. Robertson
Electron Microscopy Unit, Department of Pathology,University of Western Australia, Nedlands, Western Australia 6907, Australia
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Nichole S. Dutton
Electron Microscopy Unit, Department of Pathology,University of Western Australia, Nedlands, Western Australia 6907, Australia
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Mandy Henderson
Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, Western Australia 6009 and
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Barbara J. Chang
Department of Microbiology,
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DOI: 10.1128/IAI.68.3.1681-1686.2000
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ABSTRACT

Burkholderia pseudomallei causes melioidosis, a potentially fatal disease whose clinical outcomes include rapid-onset septicemia and relapsing and delayed-onset infections. Like other facultative intracellular bacterial pathogens, B. pseudomallei is capable of survival in human phagocytic cells, but unlike mycobacteria, Listeria monocytogenes, andSalmonella serovar Typhimurium, the species has not been reported to survive as an endosymbiont in free-living amebae. We investigated the consequences of exposing Acanthamoeba astronyxis, A. castellani, and A. polyphaga to B. pseudomallei NCTC 10276 in a series of coculture experiments. Bacterial endocytosis was observed in all three Acanthamoeba species. A more extensive range of cellular interactions including bacterial adhesion, incorporation into amebic vacuoles, and separation was observed with A. astronyxis in timed coculture experiments. Amebic trophozoites containing motile intravacuolar bacilli were found throughout 72 h of coculture. Confocal microscopy was used to confirm the intracellular location of endamebic B. pseudomallei cells. Transmission electron microscopy of coculture preparations revealed clusters of intact bacilli in membrane-lined vesicles inside the trophozoite cytoplasm; 5 × 102 CFU of bacteria per ml were recovered from lysed amebic trophozoites after 60 min of coculture. Demonstration of an interaction between B. pseudomallei and free-living acanthamebae in vitro raises the possibility that a similar interaction in vivo might affect environmental survival ofB. pseudomallei and subsequent human exposure. Endamebic passage of B. pseudomallei warrants further investigation as a potential in vitro model of intracellular B. pseudomallei infection.

  • Copyright © 2000 American Society for Microbiology
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Interaction between Burkholderia pseudomallei and Acanthamoeba Species Results in Coiling Phagocytosis, Endamebic Bacterial Survival, and Escape
Timothy J. J. Inglis, Paul Rigby, Terry A. Robertson, Nichole S. Dutton, Mandy Henderson, Barbara J. Chang
Infection and Immunity Mar 2000, 68 (3) 1681-1686; DOI: 10.1128/IAI.68.3.1681-1686.2000

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Interaction between Burkholderia pseudomallei and Acanthamoeba Species Results in Coiling Phagocytosis, Endamebic Bacterial Survival, and Escape
Timothy J. J. Inglis, Paul Rigby, Terry A. Robertson, Nichole S. Dutton, Mandy Henderson, Barbara J. Chang
Infection and Immunity Mar 2000, 68 (3) 1681-1686; DOI: 10.1128/IAI.68.3.1681-1686.2000
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KEYWORDS

Acanthamoeba
Burkholderia pseudomallei
phagocytosis

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