Article Figures & Data
Figures
- Fig. 1.
(a) NotI PFGE patterns of UTI isolates. Lanes 1 to 7, first-time UTI E. coli isolates 318-11, 366-11, 373-11, 383-11, 244-11, 298-11, and 277-11 collected from patients at University of Michigan (collection 1) ordered by time (September 1993 to November 1994); lanes 8 to 10, first-time UTI E. coliisolates 1011-11, 1030-11, and 1031-11 collected from patients at University of Texas—Austin (collection 1) ordered by time (May 1992 to August 1994). All isolates are positive for fim,aer, ompT, and kpsMT (virulence profile 100111000). (b) NotI PFGE patterns of fecal isolates. Lane 1, lambda. lanes 3 to 9, fecal E. coliisolates 400-62, 331-61, 320-62, 250-62, 157-62, 153-62, and 58-62 collected from women presenting at the University of Michigan student health service gynecology clinic over a 2-month period (collection 4). All isolates are positive for fim, aer,ompT, and kpsMT (virulence profile 100111000). Lane 2, isolate 1161-11 representing the common PFGE pattern representative of first-time UTI isolates with the same virulence profile. *, Note the difference between the PFGE patterns in lane 5 (the selected fecal driver isolate) and lane 2, one of the UTI samples with a PFGE pattern typical of the cluster that includes lane 2 in panel a (the selected UTI tester isolate).
- Fig. 2.
Location and distribution of sPCR fragments on 366-11 genome. The DNA bands are the result of the NotI PFGE pattern of strain 366-11. The identifiable bands are numbered according to their sizes. The band just above band 1 is the compression zoom. N, numbers of sPCR fragments mapped to each NotI band.
Tables
- Table 1.
Examples of sPCR probes hybridized to pathotype 5 isolates
Pathotype 5 virulence signaturea and group Hybridization [no. (%)] to probe: P5 P10 P13′ P24′ P37 P64 P68 P69 1000110000000 UTI (n = 23) 14 (61) 13 (57) 18 (78) 21 (91) 1 (4) 1 (4) 2 (9) 7 (30) Fecal (n = 27) 18 (67) 17 (63) 23 (85) 20 (74) 2 (7) 1 (4) 2 (7) 6 (22) 1001110000000b UTI (n = 15) 11 (73) 11 (73) 13 (87) 13 (87) 8 (53) 13 (87) 12 (80) 4 (27) Fecal (n = 30) 19 (63) 16 (53) 20 (67) 21 (70) 13 (43) 24 (80) 20 (67) 4 (13) 1100110000100 UTI (n = 13) 13 (100) 12 (92) 12 (92) 13 (100) 0 (0) 1 (8) 3 (23) 1 (8) Fecal (n = 19) 18 (95) 18 (95) 19 (100) 15 (79) 1 (5) 0 (0) 1 (5) 3 (16) 1101110000100 UTI (n = 25) 21 (84) 9 (36) 8 (32) 12 (48) 20 (80) 19 (76) 18 (72) 16 (64) Fecal (n = 8) 5 (63) 1 (13) 2 (25) 4 (50) 5 (63) 5 (63) 6 (75) 3 (38) All other signatures UTI (n = 18) 11 (61) 10 (56) 14 (78) 15 (83) 4 (22) 9 (50) 7 (39) 6 (33) Fecal (n = 10) 6 (60) 4 (40) 6 (60) 5 (50) 2 (20) 5 (50) 3 (30) 3 (30) Total pathotype 5 UTI (n = 94) 70 (74) 55 (59) 65 (69) 74 (79) 33 (35) 43 (46) 42 (45) 34 (36) Fecal (n = 94) 66 (70) 56 (60) 70 (74) 65 (69) 23 (24) 35 (37) 32 (34) 19 (20) Probe locationc 11 11 1 10 2 4 1 and 4 2 ↵a Hierarchically defined groups based on the associations among all genes. Those with the strongest associations combined with known biologic evidence for physical linkage were classified as a pathotype (28). 1 and 0, presence and absence, respectively, of the following genes (in order):fim, prf, sfa, aer,kpsMT, ompT, hly, cnfl,drb, capIII, papGad,prsGj96, and papGj96.
↵b Virulence signature of tester and driver strains.
↵c PFGE band of tester (366-11) DNA cleaved withNotI and hybridized.
- Table 2.
Hybridization of eight sPCR probes with genomic DNA from urinary and fecal E. coli isolates
sPCR probe Hybridization [no. (%)]a Pb UTI (n = 350) Fecal (n = 349) P13′ 222 (64) 149 (43) <0.0001 P37 206 (59) 123 (35) <0.0001 P69 113 (32) 89 (26) 0.047 P5 257 (74) 218 (63) 0.002 P10 207 (59) 129 (37) <0.0001 P24′ 268 (77) 192 (55) <0.0001 P64 203 (58) 114 (33) <0.0001 P68 218 (62) 134 (38) <0.0001
