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MOLECULAR AND CELLULAR PATHOGENESIS

Effects of Enterococcus faecalis fsrGenes on Production of Gelatinase and a Serine Protease and Virulence

Xiang Qin, Kavindra V. Singh, George M. Weinstock, Barbara E. Murray
Xiang Qin
Division of Infectious Diseases, Department of Medicine,
Department of Microbiology and Molecular Genetics, and
Center for the Study of Emerging and Re-emerging Pathogens, University of Texas Medical School, Houston, Texas 77030
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Kavindra V. Singh
Division of Infectious Diseases, Department of Medicine,
Center for the Study of Emerging and Re-emerging Pathogens, University of Texas Medical School, Houston, Texas 77030
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George M. Weinstock
Department of Microbiology and Molecular Genetics, and
Center for the Study of Emerging and Re-emerging Pathogens, University of Texas Medical School, Houston, Texas 77030
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Barbara E. Murray
Division of Infectious Diseases, Department of Medicine,
Department of Microbiology and Molecular Genetics, and
Center for the Study of Emerging and Re-emerging Pathogens, University of Texas Medical School, Houston, Texas 77030
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DOI: 10.1128/IAI.68.5.2579-2586.2000
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ABSTRACT

Three agr-like genes (fsrA,fsrB, and fsrC, for Enterococcus faecalis regulator) were found upstream of the previously reported gelatinase gene (gelE) and a downstream putative serine protease gene (sprE; accession number Z12296 ) ofEnterococcus faecalis OG1RF. The deduced amino acid sequence of fsrA shows 26% identity and 38% similarity toStaphylococcus aureus AgrA (the response regulator of the accessory gene regulator system in the agr locus), FsrB shows 23% identity and 41% similarity to S. aureus AgrB, and FsrC shows 23% identity and 36% similarity to S. aureus AgrC (the sensor transducer of Agr system). Northern blot analysis suggested that gelE and sprE are cotranscribed and that fsrB and fsrC are also cotranscribed in OG1RF. Northern blot analysis of fsrA,fsrB, fsrC, gelE, andsprE insertion mutants showed that fsrB,fsrC, gelE, and sprE are not expressed in fsrA, fsrB, and fsrCmutants, while insertion in an open reading frame further upstream offsrA did not effect the expression of these genes, suggesting that agr-like genes may be autoregulated and that they regulate gelE and sprE expression, as further confirmed by complementation of fsr gene mutations with a 6-kb fragment which contains all three fsr genes in the shuttle vector, pAT18. Testing of 95 other isolates of E. faecalis showed that 62% produced gelatinase (Gel+), while 91% (including all Gel+ strains) hybridized to agelE probe; 71% (including all Gel+ strains) hybridized to an fsr probe, corroborating the conclusion that both gelE and fsr are necessary for gelatinase production. Testing of fsrA, fsrB, and sprE mutants in a mouse peritonitis model showed that sprE and agr-like gene mutants resulted in highly significantly prolonged survival compared to the parent strain OG1RF, a finding similar to what we had previously shown for agelE mutant. These results suggest that sprEand agr-like genes contribute to the virulence of E. faecalis OG1RF in this model.

  • Copyright © 2000 American Society for Microbiology
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Effects of Enterococcus faecalis fsrGenes on Production of Gelatinase and a Serine Protease and Virulence
Xiang Qin, Kavindra V. Singh, George M. Weinstock, Barbara E. Murray
Infection and Immunity May 2000, 68 (5) 2579-2586; DOI: 10.1128/IAI.68.5.2579-2586.2000

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Effects of Enterococcus faecalis fsrGenes on Production of Gelatinase and a Serine Protease and Virulence
Xiang Qin, Kavindra V. Singh, George M. Weinstock, Barbara E. Murray
Infection and Immunity May 2000, 68 (5) 2579-2586; DOI: 10.1128/IAI.68.5.2579-2586.2000
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KEYWORDS

Bacterial Proteins
DNA-Binding Proteins
Dihydropteridine Reductase
Enterococcus faecalis
Escherichia coli Proteins
Gelatinases
Hemeproteins
NADH, NADPH Oxidoreductases
Serine Endopeptidases
Trans-Activators

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