Article Figures & Data
Figures
- Fig. 1.
Mice were given 6 × 109 CFU ofM. avium 101 i.v. Four to 11 mice were used for blood culture and 3 to 7 mice were used for brain culture at each timepoint. Data are the mean CFU ± SEM. Mice had a significant increase in bacterial load in the brain within the first 2 months (P = 0.0058).
- Fig. 2.
Brain histopathology, showing intense infiltration of lymphocytes and macrophages in the optical chiasm 2 weeks after i.c. infection.
- Fig. 3.
Brain histopathology of mice receiving 3 × 108 CFU i.v. (A) At 1 month after inoculation, one nonnecrotizing granuloma was seen; HE strain. (B) Perivascular cuffing seen 2 months after inoculation. HE stain. (C) mild choroid plexus infiltration. HE stain. (D) acid-fast bacilli in the parenchyma of the choroid plexus. AFB stain. (E and F) At 4 months after infection there was cellular infiltration along the ventricle (E) (HE stain) and acid-fast bacilli were found in the lesion (F) (AFB stain). (G and H) At 6 months after inoculation, meningeal granuloma with foamy histiocytes was found (G) (HE stain) containing numerous acid-fast bacilli (H) (AFB stain). Magnifications: A and C, ×100; B, ×400; D, ×630; E and G, ×250; F and H, ×600.
Tables
- Table 1.
Bacterial load in blood and brain after infection by different routes
Time postinfection Sample Mean no. of bacteriaa (log CFU) (n = 3) 107 CFU i.v. 108 CFU i.v. 109 CFU i.v. 104 CFU i.c. 2 h Blood 4.88 5.47 >6.24 Brain 0 0 0 2.13 1 day Blood 3.80 4.78 4.58 Brain 2.88 3.27 2.95 2.78 7 days Blood 4.62 3.38 4.32 Brain 3.151 3.88 4.01 3.381 14 days Blood 2.56 2.65 4.88 Brain 4.301,2 3.951 4.181 4.011,2 28 days Blood <1.8 4.34 5.30 Brain 3.651 4.561,2,3 4.691,2 4.511,2 56 days Blood 2.36 4.04 3.71 Brain 2.87 4.681,2,3 4.691,2 3.40 ↵a 1, P < 0.05 compared with the number of bacteria in the brain at day 1; 2, P < 0.05 compared with the number of bacteria in the brain at day 7; 3, P < 0.05 compared with the number of bacteria in the brain at day 14.
- Table 3.
Comparison of the number of M. avium in the brains of wild-type and CD18 KO mice after i.v. infectiona
Day postinfection 105 CFU/g of brain (mean ± SEM, n = 10) Wild type CD18 KO 1 1.2 ± 0.4 1.6 ± 0.2 7 2.4 ± 0.3 2.7 ± 0.3 14 3.5 ± 0.4 3.9 ± 0.1 28 3.3 ± 0.6 4.0 ± 0.5 ↵a P > 0.05 for all the comparisons between number of bacteria in wild type and CD18 KO at each timepoint.
