Article Figures & Data
Figures
- Fig. 1.
Colony blots showing hybridization of B. abortus ST mutants with probes containing tags amplified from the input pool and from output pools recovered from spleens of three mice at 8 weeks postinfection. At the upper left and lower right corner of each blot is the parent stain, B. abortus 2308. Arrows indicate mutants (BA142, BA152, BA159, and BA184 [left to right]) which gave weak signals in the output pools of at least two of the three mice inoculated and whose competitive infection defect was confirmed subsequently. Open squares indicate mutants that gave inconsistent hybridization results and were therefore eliminated from the screen.
- Fig. 2.
Mutants with statistically significant colonization defects, as confirmed by individual competitive infections and statistical analysis. Homologues of disrupted genes are given in parentheses. nh, no homology.
Tables
- Table 1.
Confirmation of competitive defects of STM mutants by competitive infection of mice with B. abortus S2308a
Mutant Competitive index Mutant Competitive index in vivo at 8 wk In vivo In vitro 2 wk 8 wk BA11 2.29* 1.86 BA18 0.064 BA41 4.74* >29,512*** 0.97 BA27 2.24 BA73 3.36 147.9* 2.74 BA31 3.63* BA87 1.08 10.47** 0.79 BA38 7.76* BA100 20.89*** >758.6*** 4.70 BA63 1.86* BA102 1.47 93.3*** 1.80 BA70 3.39 BA109 0.92 3.21 BA99 1.02 BA114 133.07** >1,047.1*** 0.91 BA105 2.00 BA157 0.74 1.58 BA119 6.31 BA159 1.50 23.44* 2.20 BA116 2.51 BA184 1,306.8*** >2,168.2*** 0.57 BA122 95.50* BA130 3.02 BA138 3.72 BA140 1.20 BA142 70.79*** BA152 72.44*** ↵a Competitive index was calculated as [(CFUS2308/CFUmutant) recovered/(CFUS2308/CFUmutant) inoculated]. Significant differences between competitive indices of STM mutants and a control, nonattenuated mutant were compared using a Studentt test. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.005.
- Table 2.
Characterization of B. abortus genes identified by STM
Mutant Closest homologue (organism) GenBank accession no. Inferred function BA41 virB1 (B. suis) AF141604 Type IV secretion BA114 virB10 (B. suis) AF141604 Type IV secretion BA184 wbkA (B. melitensis) AF047478 O antigen biosynthesis BA159 gluP (B. abortus) U43785 Uptake of glucose and galactose BA102 P protein (Synechocystissp.) D90914 Glycine dehydrogenase gcvB(M. tuberculosis) Q50601 BA152 gltD(R. etli) AF107264 Glutamate synthase BA87 HP1225 (Helicobacter pylori) AE000628 Conserved inner membrane protein BA73 No homology ?
