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Bacterial Infections

Identification of Genes Required for Chronic Persistence of Brucella abortus in Mice

Priscilla C. Hong, Renée M. Tsolis, Thomas A. Ficht
Priscilla C. Hong
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4467
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Renée M. Tsolis
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4467
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Thomas A. Ficht
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4467
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DOI: 10.1128/IAI.68.7.4102-4107.2000
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    Fig. 1.

    Colony blots showing hybridization of B. abortus ST mutants with probes containing tags amplified from the input pool and from output pools recovered from spleens of three mice at 8 weeks postinfection. At the upper left and lower right corner of each blot is the parent stain, B. abortus 2308. Arrows indicate mutants (BA142, BA152, BA159, and BA184 [left to right]) which gave weak signals in the output pools of at least two of the three mice inoculated and whose competitive infection defect was confirmed subsequently. Open squares indicate mutants that gave inconsistent hybridization results and were therefore eliminated from the screen.

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    Fig. 2.

    Mutants with statistically significant colonization defects, as confirmed by individual competitive infections and statistical analysis. Homologues of disrupted genes are given in parentheses. nh, no homology.

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  • Table 1.

    Confirmation of competitive defects of STM mutants by competitive infection of mice with B. abortus S2308a

    MutantCompetitive indexMutantCompetitive index in vivo at 8 wk
    In vivoIn vitro
    2 wk8 wk
    BA112.29*1.86BA180.064
    BA414.74*>29,512***0.97BA272.24
    BA733.36147.9*2.74BA313.63*
    BA871.0810.47**0.79BA387.76*
    BA10020.89***>758.6***4.70BA631.86*
    BA1021.4793.3***1.80BA703.39
    BA1090.923.21BA991.02
    BA114133.07**>1,047.1***0.91BA1052.00
    BA1570.741.58BA1196.31
    BA1591.5023.44*2.20BA1162.51
    BA1841,306.8***>2,168.2***0.57BA12295.50*
    BA1303.02
    BA1383.72
    BA1401.20
    BA14270.79***
    BA15272.44***
    • ↵a Competitive index was calculated as [(CFUS2308/CFUmutant) recovered/(CFUS2308/CFUmutant) inoculated]. Significant differences between competitive indices of STM mutants and a control, nonattenuated mutant were compared using a Studentt test. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.005.

  • Table 2.

    Characterization of B. abortus genes identified by STM

    MutantClosest homologue (organism)GenBank accession no.Inferred function
    BA41virB1 (B. suis)AF141604Type IV secretion
    BA114virB10 (B. suis)AF141604Type IV secretion
    BA184wbkA (B. melitensis)AF047478O antigen biosynthesis
    BA159gluP (B. abortus)U43785Uptake of glucose and galactose
    BA102P protein (Synechocystissp.)D90914Glycine dehydrogenase
    gcvB(M. tuberculosis)Q50601
    BA152gltD(R. etli)AF107264Glutamate synthase
    BA87HP1225 (Helicobacter pylori)AE000628Conserved inner membrane protein
    BA73No homology?
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Identification of Genes Required for Chronic Persistence of Brucella abortus in Mice
Priscilla C. Hong, Renée M. Tsolis, Thomas A. Ficht
Infection and Immunity Jul 2000, 68 (7) 4102-4107; DOI: 10.1128/IAI.68.7.4102-4107.2000

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Identification of Genes Required for Chronic Persistence of Brucella abortus in Mice
Priscilla C. Hong, Renée M. Tsolis, Thomas A. Ficht
Infection and Immunity Jul 2000, 68 (7) 4102-4107; DOI: 10.1128/IAI.68.7.4102-4107.2000
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KEYWORDS

Brucella abortus
brucellosis
Genes, Bacterial

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