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MOLECULAR AND CELLULAR PATHOGENESIS

Identification of a Novel Gene Cluster Encoding Staphylococcal Exotoxin-Like Proteins: Characterization of the Prototypic Gene and Its Protein Product, SET1

Rachel J. Williams, John M. Ward, Brian Henderson, Stephen Poole, Bernard P. O'Hara, Michael Wilson, Sean P. Nair
Rachel J. Williams
Cellular Microbiology Research Group, Division of Surgical Sciences,
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John M. Ward
Department of Biochemistry and Molecular Biology, and
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Brian Henderson
Cellular Microbiology Research Group, Division of Surgical Sciences,
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Stephen Poole
Division of Endocrinology, National Institute for Biological Standards and Control, Potters Bar,United Kingdom
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Bernard P. O'Hara
Department of Biochemistry and Molecular Biology, and
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Michael Wilson
Microbiology Department, Eastman Dental Institute, University College London, London, and
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Sean P. Nair
Cellular Microbiology Research Group, Division of Surgical Sciences,
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DOI: 10.1128/IAI.68.8.4407-4415.2000
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ABSTRACT

We report the discovery of a novel genetic locus withinStaphylococcus aureus that encodes a cluster of at least five exotoxin-like proteins. Designated the staphylococcal exotoxin-like genes 1 to 5 (set1 to set5), these open reading frames have between 38 and 53% homology to each other. All five proteins contain consensus sequences that are found in staphylococcal and streptococcal exotoxins and toxic shock syndrome toxin 1 (TSST-1). However, the SETs have only limited overall sequence homology to the enterotoxins and TSST-1 and thus represent a novel family of exotoxin-like proteins. The prototypic gene in this cluster,set1, has been cloned and expressed. Recombinant SET1 stimulated the production of interleukin-1β, interleukin-6, and tumor necrosis factor alpha by human peripheral blood mononuclear cells. PCR analysis revealed that set1 was distributed among other strains of S. aureus but not in the other staphylococcal species examined. Sequence analysis of the set1 genes from different strains revealed at least three allelic variants. The protein products of these allelic variants displayed a 100-fold difference in their cytokine-inducing potency. The distribution of allelic variants of the set genes among strains of S. aureus may contribute to differences in the pathogenic potential of this bacterium.

  • Copyright © 2000 American Society for Microbiology
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Identification of a Novel Gene Cluster Encoding Staphylococcal Exotoxin-Like Proteins: Characterization of the Prototypic Gene and Its Protein Product, SET1
Rachel J. Williams, John M. Ward, Brian Henderson, Stephen Poole, Bernard P. O'Hara, Michael Wilson, Sean P. Nair
Infection and Immunity Aug 2000, 68 (8) 4407-4415; DOI: 10.1128/IAI.68.8.4407-4415.2000

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Identification of a Novel Gene Cluster Encoding Staphylococcal Exotoxin-Like Proteins: Characterization of the Prototypic Gene and Its Protein Product, SET1
Rachel J. Williams, John M. Ward, Brian Henderson, Stephen Poole, Bernard P. O'Hara, Michael Wilson, Sean P. Nair
Infection and Immunity Aug 2000, 68 (8) 4407-4415; DOI: 10.1128/IAI.68.8.4407-4415.2000
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KEYWORDS

bacterial toxins
Genes, Bacterial
Multigene Family
Staphylococcus aureus

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