Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Infection and Immunity
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Bacterial Infections

Experimental Model of Human Body Louse Infection Using Green Fluorescent Protein-Expressing Bartonella quintana

Pierre-Edouard Fournier, Michael F. Minnick, Hubert Lepidi, Eric Salvo, Didier Raoult
Pierre-Edouard Fournier
Unité des rickettsies, CNRS:UPRESA 6020, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael F. Minnick
Division of Biological Sciences, University of Montana, Missoula, Montana 59812-1002
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hubert Lepidi
Unité des rickettsies, CNRS:UPRESA 6020, and
Laboratoire d'Histologie, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric Salvo
Unité des rickettsies, CNRS:UPRESA 6020, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Didier Raoult
Unité des rickettsies, CNRS:UPRESA 6020, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/IAI.69.3.1876-1879.2001
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Experimental model of body louse infection by GFP-expressing B. quintana. (a) Pediculus humanus humanus feeding on a rabbit; (b) fluorescent colony isolated on selective agar from an infected louse 10 days after the initial infection; (c) autofluorescence of body lice; (d) immunohistological detection of B. quintana. Note the numerous erythrocytes and the clusters of bartonellae in the intestine lumen (red clumps) and against the intestine wall (blue). Streptavidin-biotin-peroxidase method, polyclonal rabbit anti-B. quintana used at a dilution of 1:400, hemalun counterstain. Magnification, ×660.

  • Fig. 2.
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Comparison of the survival rate over time of 200 lice infected with B. quintana and 200 control lice. Day 0, day of infection.

Tables

  • Figures
  • Table 1.

    Strains and plasmids used in this study

    Strain or plasmid Genotype or relevant characteristicsReference or source
    Strains
     E. coliDH5 F−(Φ80dlacZΔM15) Δ(lacZY AargF)U169 deoR recA1 endA1 hsdR17 (rK− mK+),supE44 λ-thi-1 gyrA96 relA1 λ-CH616Gibco-BRL (7)
     B. quintana‘Oklahoma’
    Plasmids
     pBBR1-MCS2 Kmr Mob Rep origin, Bartonella shuttle vector Michael Kovach (7)
     pFPV25 Source of promoterless GFP (gfpmut3a)Raphael Valdivia
     PJMBGFP PBBR1-MCS2 with a 970-bpHindIII fragment containing B. bacilliformisflagellin promoter (fla-pro) upstream ofgfpmut3aJames M. Battitsti (2)
PreviousNext
Back to top
Download PDF
Citation Tools
Experimental Model of Human Body Louse Infection Using Green Fluorescent Protein-Expressing Bartonella quintana
Pierre-Edouard Fournier, Michael F. Minnick, Hubert Lepidi, Eric Salvo, Didier Raoult
Infection and Immunity Mar 2001, 69 (3) 1876-1879; DOI: 10.1128/IAI.69.3.1876-1879.2001

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Infection and Immunity article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Experimental Model of Human Body Louse Infection Using Green Fluorescent Protein-Expressing Bartonella quintana
(Your Name) has forwarded a page to you from Infection and Immunity
(Your Name) thought you would be interested in this article in Infection and Immunity.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Experimental Model of Human Body Louse Infection Using Green Fluorescent Protein-Expressing Bartonella quintana
Pierre-Edouard Fournier, Michael F. Minnick, Hubert Lepidi, Eric Salvo, Didier Raoult
Infection and Immunity Mar 2001, 69 (3) 1876-1879; DOI: 10.1128/IAI.69.3.1876-1879.2001
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • ACKNOWLEDGMENTS
    • Notes
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Bartonella quintana
Luminescent Proteins
Pediculus

Related Articles

Cited By...

About

  • About IAI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #IAIjournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0019-9567; Online ISSN: 1098-5522