Article Figures & Data
Figures
- Fig. 1.
Experimental model of body louse infection by GFP-expressing B. quintana. (a) Pediculus humanus humanus feeding on a rabbit; (b) fluorescent colony isolated on selective agar from an infected louse 10 days after the initial infection; (c) autofluorescence of body lice; (d) immunohistological detection of B. quintana. Note the numerous erythrocytes and the clusters of bartonellae in the intestine lumen (red clumps) and against the intestine wall (blue). Streptavidin-biotin-peroxidase method, polyclonal rabbit anti-B. quintana used at a dilution of 1:400, hemalun counterstain. Magnification, ×660.
- Fig. 2.
Comparison of the survival rate over time of 200 lice infected with B. quintana and 200 control lice. Day 0, day of infection.
Tables
- Table 1.
Strains and plasmids used in this study
Strain or plasmid Genotype or relevant characteristics Reference or source Strains E. coliDH5 F− (Φ80dlacZΔM15) Δ(lacZY AargF)U169 deoR recA1 endA1 hsdR17 (rK − mK +),supE44 λ-thi-1 gyrA96 relA1 λ-CH616 Gibco-BRL (7) B. quintana ‘Oklahoma’ Plasmids pBBR1-MCS2 Kmr Mob Rep origin, Bartonella shuttle vector Michael Kovach (7) pFPV25 Source of promoterless GFP (gfpmut3a) Raphael Valdivia PJMBGFP PBBR1-MCS2 with a 970-bpHindIII fragment containing B. bacilliformisflagellin promoter (fla-pro) upstream ofgfpmut3a James M. Battitsti (2)
